|Psychological Stress and Salivary Cortisol Levels in Patients with Plaque Psoriasis
|In this study, psoriasis was associated with higher psychological stress, anxiety and depressive symptoms, and with impaired cortisol response to stress. MassChrom Cortisol in Saliva was used.
|PKA Cα subunit mutation triggers caspase-dependent RIIβ subunit degradation via Ser114 phosphorylation
|These findings reveal the molecular mechanisms and pathophysiological relevance of the R subunit degradation caused by PRKACA mutations, adding another dimension to the deregulation of PKA signaling caused by PRKACA mutations in adrenal Cushing’s syndrome. Steroid hormones in cell culture supernatants were measured by MassChrom assay.
|Application Note: Determination of Cortisol and Cortisone in Urine with LC-MS/MS
|This application note describes a protocol for the determination of cortisol and cortisone in urine with Chromsystems components. Intra- and interassay and (analytical) measurement range are satisfactory for the analysis of human urine samples.
|Characteristics of preoperative steroid profiles and glucose metabolism in patients with primary aldosteronism developing adrenal insufficiency after adrenalectomy
|The study's findings support the notion that glucocorticoid co-secretion from aldosterone-producing adenomas might be a risk factor for the development of adrenal insufficiency after adrenalectomy. Furthermore, the data demonstrate that correlation- and classification models were improved by inclusion of data from LC–MS/MS based steroid profiles. MassChrom Steroids was used.
|Requirement for oxytocin augmentation in spontaneous parturition is associated with the maternal serum steroid hormones assessed by liquid chromatography coupled to the tandem mass spectrometry
|This research provided reference ranges for the selected maternal serum steroid hormones at the beginning of parturition. Association of corticosterone, dehydroepiandrosterone, and androstenedione with the need for the oxytocin infusion usage has been established. Dehydroepiandrosterone could be potential predictor of oxytocin infusion augmentation for progression of the parturition. Steroid hormones were measured with MassChrom Steroids
|A pilot study: Relationship between Bisphenol A, Bisphenol A glucuronide and sex steroid hormone levels in cord blood in A South African population
|The findings of the study indicate a significant relationship between some key sex steroid hormones namely testosterone, dihydrotestosterone and estradiol and fetal exposure BPA. MassChrom Steroids assay was used for the determination of several steroind hormones.
|Testosterone serum levels are not predictive of maternal virilization in hyperreactio luteinalis
|Maternal virilization in hyperreactio luteinalis is a rare, self-limited condition. Interventions during pregnancy are usually not necessary unless surgical complications, do occur. Virilization cannot be predicted by the level of circulating testosterone. Androstenedione, progesterone, cortisol, dehydroepiandrosterone sulfate (DHEAS), estradiol, 17-OH-progesterone and testosterone measured with MassChrom Steroids.
|Active steroid hormone synthesis renders adrenocortical cells highly susceptible to type II ferroptosis induction
|This report is the first to demonstrate extraordinary sensitivity of adrenal cortex cells to ferroptosis dependent on their active steroid synthetic pathways. Mitotane does not induce this form of cell death in ACC cells. Measured with MassChrom Steroids.
|Androgens predispose males to monocyte-mediated immunopathology by inducing the expression of leukocyte recruitment factor CXCL1
|The authors characterized the immune response underlying a classical, male-biased parasitic disease and identified a prominent role of classical monocytes expressing a typical cytokine set. Hormone analysis was performed with MassChrom Steroids.
|Global 11 Beta-Hydroxysteroid Dehydrogenase Activity Assessed by the Circulating Cortisol to Cortisone Ratio Is Associated With Features of Metabolic Syndrome
|The global index of 11HSD activity, assessed by the circulating cortisol:cortisone ratio, was related to high blood pressure and fasting plasma glucose and may serve as a proxy to global 11HSD activity. Measured with MassChrom Steroids assay.
|High DHEAS Levels in Girls Is Associated withEarlier Pubertal Maturation and Mild Increase inAndrogens throughout Puberty without AffectingPostmenarche Ovarian Morphology
|Prepubertal DHEAS in normal girls is associated with earlier thelarche, pubarche, and menarche, and a mild androgen increase throughout
puberty. . Concentrations of DHEAS, androstenedione17-OH progesterone (17-OHP), and testosterone were analyzed by MassChrom Steroids assay.
|Effects of interleukin-1 antagonism on cortisollevels in individuals with obesity: a randomizedclinical trial
|MassChrom Steroids assay was used to determine cortisol in a clinical study on IL-1 antagonism.
|Abiraterone Acetate for Cushing Syndrome: Study in a Canine Primary Adrenocortical Cell Culture Model
|Primary canine adrenocortical cell culture may be a useful model system for drug testing. For the treatment of Cushings syndrome (CS), AA may to be superior to other steroidogenesis inhibitors due to its low toxicity. Steroids were measured with the MassChrom Steroids assay.
|Dihydrotestosterone is a predictor for mortality in males with community-acquired pneumonia: results of a 6-year follow-up study
|This clinical study invesigtates dihydrotestosterone as a predictor for mortality of pneumonia. aldosterone, progesterone, OH-progesterone, DHEA, DHEA-S, testosterone, androstenedione, and dihydrotestosterone are measured with MassChrom Steroids
|Preoperative treatment with metyrapone in patients with Cushing’s syndrome due to adrenal adenoma: a pilot prospective study
|This prospective pilot study demonstrated that metyrapone is effective in normalizing biochemical and clinical parameters in patients with CS due to adrenal adenoma before surgical intervention, with minimal side effects.. cortisol, 11-deoxycortisol, DHEAS, testosterone and androstenedione levels by LC–MS/MS with MassChrom Steroids
|Effects of Nandrolone Stimulation on Testosterone Biosynthesis in Leydig Cells
|results support the hypothesis that nandrolone is able to modify testosterone production by interfering with StAR and CYP17A1 expression in Leydig cells. MassChrom Steroids was used for the determination of steroid hormones.
|Measurement of Serum Testosterone in Nondiabetic Young Obese Men: Comparison of Direct Immunoassay to Liquid Chromatography-Tandem Mass Spectrometry
|The study concludes that the determination of testosterone by immunoassay in nondiabetic young men with obesity
yields lower concentrations than by LC-MS/MS, resulting in an equivocal increased diagnosis of hypoandrogenemia, which could lead to inaccurate diagnosis and unnecessary treatment. MassChrom Steroids was used.
|Hyperandrogenism by Liquid Chromatography Tandem Mass Spectrometry in PCOS: Focus on Testosterone and Androstenedione
|The results confirm that LC-MS/MS is more sensitive than immunoassays in the diagnosis of PCOS with LC-MS/MS detecting higher levels of testosterone and androstenedione. Furthermore testosterone by LC-MS/MS correlates with adverse metabolic parameters. MassChrom Steroids was used for the determination of testosterone and androstenedione.
|Global 11 Beta-Hydroxysteroid Dehydrogenase Activity Assessed by the Circulating Cortisol to Cortisone Ratio is Associated with Features of Metabolic Syndrome
|The global index of 11HSD activity, assessed by the circulating cortisol:cortisone ratio, was related to high blood pressure and fasting plasma glucose and may serve as a proxy to global 11HSD activity. Serum cortisol and cortisone were measured with MassChrom Steroids.
|Analytical bias of automated immunoassays for six serum steroid hormones assessed by LC-MS/MS
|Evaluated immunoassays failed to satisfy requirements for methods’ comparability and produced significant analytical biases in respect to the LC-MS/MS assay, especially at low concentrations. LC-MS/MS assay used was MassChrom Steroids
|Mass spectrometry reveals misdiagnosis of primary aldosteronism with scheduling for adrenalectomy due to immunoassay interference
|Although the patient may represent an isolated case of primary aldosteronism, immunoassay interference leading to misdiagnosis of primary aldosteronism, unnecessary AVS and potentially wrongful removal of an adrenal, it is also possible that such inaccuracies may impact the diagnostic process and treatment for other patients. Measured with MassChrom Steroids and compared with other assays.
Please find here publications as guides for reference ranges or therapeutic ranges. They may differ from other published data and are only intended to serve as a rough guide. As data vary depending on patient population and measurement method, please determine ranges for your laboratory. When determining ranges make sure that you comply with local national requirements.
|Thomas L. (ed)- Labor und Diagnose, 8th edition, TH books (2012)
|Book with reference values
|Shackleton C. (2008) Genetic disorders of steroid metabolism diagnosed by mass spectrometry. In: Blau N Duran M Gibson KM (eds.) Laboratory guide to the methods in biochemical genetics p. 549-606
|Reference values for DHT
|Soldin SJ, Wang, et al. Pediatric Refrence Intervals: 7th edition, AACC Press (2011)
|Reference values for several steroids including adrostenedione, DHEA, DHT