Parameter Set Antiepileptic Drugs - LC-MS/MS

Order No.: 92921
Parameters:
10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide

Encompasses 26 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A
Method for automated sample preparation also available

Carbamazepine

Carbamazepine-10,11-epoxide

10,11-Dihydroxycarbamazepine

10-OH-Carbamazepine

Oxcarbamazepine

Ethosuximide

Felbamate

Gabapentin

Lacosamide

Lamotrigine

Levetiracetam (Keppra®)

N-Desmethylmesuximide

Phenobarbital

Phenylethylmalonamide (PEMA)

Phenytoin

Pregabalin

Primidone

Rufinamide

 

 

Stiripentol

Sultiame

Theophylline

Tiagabine

Topiramate

Valproic acid

Vigabatrin

Zonisamid

Clinical relevance

Epileptic seizures are the result of synchronous discharges of neuron groups in the brain that can lead to sudden and involuntary stereotypical behavioural or sensory disorders. Numerous types of seizures have been described, each of which requires specialised therapy. The probability of them occurring depends on a number of factors. In addition to genetic predisposition, it is primarily exogenous factors that are relevant, such as accident, thrombosis, tumours or meningitis. Therapy with anticonvulsive medication (antiepileptics) leads to a reduction in seizures and sometimes even a complete elimination of seizures in most treated patients. The precondition for the antiepileptics to have a therapeutic effect is usually patient compliance, which means regular use of the medication. Thus, monitoring of the blood levels is essential, especially during initial dose setting.

 

Product advantages

With the Parameter Set Antiepileptics in serum/plasma, 26 different drugs can be measured quickly and efficiently using LC-MS/MS. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and the reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets.

For the analysis you require the MassTox® TDM Basic Kit A, the specific MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.

More Information
Limit of quantification 0.1 – 7.0 mg/l
Linearity 1 – 300 mg/l
Recovery 84 – 115 %
Intraassay CV = 2 – 8 %
Interassay CV = 4 – 9 %
Analysis Time 2.5 – 3.5 min
Sample Preparation
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent (= mixture A).
  • Pipette 50 µl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system.
Sample Stability Samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.
Specimen Serum/Plasma
Injection Volume 0.2 – 50 µl
Gradient

Starting point: 0% Mobile Phase 2

Group 1
0.00→2.00 min, 100 % Mobile Phase 2
2.00-2.60 min, 100 % Mobile Phase 2
2.61-3.50 min, 0 % Mobile Phase 2

Group 2 and 3
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.60 min, 100 % Mobile Phase 2
1.61-2.50 min, 0 % Mobile Phase 2

Group 4 and 5
0.00→1.00 min, 100 % Mobile Phase 2
1.00-1.80 min, 100 % Mobile Phase 2
1.81-2.70 min, 0 % Mobile Phase 2

Ionisation ESI positive and negative
MS/MS-Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 10-OH-Carbamazepine, Carbamazepine, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, N-Desmethylmesuximide, Phenobarbital, Phenytoin, Pregabalin, Primidone, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide
  1. 92546 internal standard mix antiepileptic drugs
    Internal Standard Mix Antiepileptic Drugs
    Order no.: 92546
    Component of the Parameter Set Antiepileptic Drugs, available separately
    Details
  2. 0249XT 0250XT 0251XT LCMS TDM Series A controls antiepileptic drugs
    Details
  1. 0249XT 0250XT 0251XT LCMS TDM Series A controls antiepileptic drugs
    Details
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® TDM Series A

    Details
  2. 92034XT LCMS TDM Series A tuning mix
    Tuning Mix Antiepileptic Drugs/EXTENDED 1
    Order no.: 92034/XT
    Tuning Mix 1 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
    Details
  3. 92035XT LCMS TDM Series A tuning mix
    Tuning Mix Antiepileptic Drugs/EXTENDED 2
    Order no.: 92035/XT
    Tuning Mix 2 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
    Details
  4. 92036XT LCMS TDM Series A tuning mix
    Tuning Mix Antiepileptic Drugs/EXTENDED 3
    Order no.: 92036/XT
    Tuning Mix 3 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
    Details
  5. 92037XT LCMS TDM Series A tuning mix
    Tuning Mix Antiepileptic Drugs/EXTENDED 4
    Order no.: 92037/XT
    Tuning Mix 4 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
    Details
  6. 92038XT LCMS TDM Series A tuning mix
    Tuning Mix Antiepileptic Drugs/EXTENDED 5
    Order no.: 92038/XT
    Tuning Mix 5 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
    Details
  7. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  8. PEEK-encased Prefilter, 2 µm
    Details
As a customer please login or register to gain full access.