Amino Acid Analysis (AAA)
The MassChrom® Amino Acid Analysis (AAA) assays in plasma/serum or urine offer the full spectrum for your amino acid analysis. You can quantify 48 amino acids in plasma/serum. Or you opt to quantify 52 amino acids and creatinine in urine in one run. Both assays are used, among other things, for the determination of metabolic diseases.
- One chromatographic platform for plasma/serum and urine
- Fast sample preparation without derivatisation
- Short run times
- Excellent separation of all isobaric compounds
- Each analyte with its own internal standard
- Reaction vials or 96 deep well plates
Assays for Quantitative Amino Acid Analysis
MassChrom® Amino Acid Analysis in Plasma/Serum
|Order No.||Method||Number of Tests
|75111||LC-MS/MS||3 x 96||Assay with reaction vials|
|75111/DWP||LC-MS/MS||3 x 96||Assay with 96 deep well plates|
Flexible Analysis – One Assay 2 Test Modes
Benefit from the flexibility of the Chromsystems assay in plasma/serum: You can either measure 48 amino acids within 20 minutes, or alternatively determine the PKU/MSUD panel within 8 minutes - the choice is yours. The analytes measured with both, the Full Panel and the PKU/MSUD Panel, are leucine, allo-isoleucine, isoleucine, methionine, phenylalanine, tyrosine and valine. Both panels use the identical chromatographic set-up and sample preparation.
MassChrom® Amino Acid Analysis in Urine
|Order no,||Method||Number of Tests||Characteristics|
|75222||LC-MS/MS||3 x 96||Assay with reaction vials|
|75222/DWP||LC-MS/MS||3 x 96||Assay with 96 deep well plates|
52 Amino Acids and Creatinine in One Run
This assay allows the quantitative determination of 52 amino acids/metabolites and creatinine by LC-MS/MS within 20 minutes. Creatinine is also measured within the same run, which is used for the normalisation of the urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination and subsequent dilution of the sample. The assay can be used for a number of applications, such as the determination of renal aminoaciduria, and can be ideally combined with our plasma assay without needing to change the chromatographic set-up.
All Parameters at a Glance
* These additional 5 parameters are included in the urine assay.
Dr Rachel Carling, Scientific Director for Viapath, reports on a study that compares the current gold standard in quantitative amino acid analysis (AAA) - ion exchange chromatography (IEC) with LC-MS/MS.
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