Aminosäuren-Analytik - ChromsystemsAminosäuren-Analytik - Chromsystems

Amino Acid Analysis (AAA)

The MassChrom® Amino Acid Analysis (AAA) assays in plasma/serum or urine offer a wide spectrum for your amino acid analysis. You can quantify 48 amino acids in plasma/serum. Or you opt to quantify 52 amino acids and creatinine in urine in one run. Both assays are intended, among other things, for patients with suspected metabolic diseases.

  • One chromatographic platform for plasma/serum and urine
  • Fast sample preparation without derivatisation
  • Shorter run times than HPLC methods
  • Excellent separation of all isobaric analytes
  • Each analyte with its own internal standard
  • Reaction vials or 96 deep well plates
CSQUANTCSQUANT

Assays for Quantitative Amino Acid Analysis

MassChrom® Amino Acid Analysis in Plasma/Serum

Order No. Method Number of Tests
Characteristics
75111 LC-MS/MS 3 x 96 Assay with reaction vials
75111/DWP LC-MS/MS 3 x 96 Assay with 96 deep well plates
Flexible Analysis: One Assay – 2 Test Modes

Benefit from the flexibility of the Chromsystems assay in plasma/serum: You can either measure 48 amino acids within 20 minutes, or alternatively determine the PKU/MSUD panel within 9 minutes - the choice is yours. The analytes measured with both, the Full Panel and the PKU/MSUD Panel, are leucine, allo-isoleucine, isoleucine, methionine, phenylalanine, tyrosine and valine. Both panels use the identical chromatographic set-up and sample preparation.

MassChrom® Amino Acid Analysis in Urine

Order no, Method Number of Tests Characteristics
75222 LC-MS/MS 3 x 96 Assay with reaction vials
75222/DWP LC-MS/MS 3 x 96 Assay with 96 deep well plates
52 Amino Acids and Creatinine in One Run

This assay allows the quantitative determination of 52 amino acids/metabolites by LC-MS/MS within 20 minutes. Creatinine is also measured within the same run, which is used for the normalisation of the urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination and subsequent dilution of the sample. The assay can be used for a number of applications, such as the determination of renal aminoacidurias, and can be ideally combined with our plasma assay without needing to change the chromatographic set-up.


All Parameters at a Glance

Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine
Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine-S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine
Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Kynurenine*
Hydroxykynurenine*
Leucine
Allo-Isoleucine
Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Oxoproline/Pyroglutamic acid*
Phenylacetylglutamine*
Phenylalanine
Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

Creatinine*

* These 5 additional parameters can only be measured with the urine assay.


Dr Rachel Carling, Scientific Director for Viapath, reports on a study that compares the current gold standard in quantitative amino acid analysis (AAA) - ion exchange chromatography (IEC) with LC-MS/MS.


 

Download Brochure

You need all the facts at a glance? Then you are welcome to download our brochures. After you have filled out the following form, the respective links to download your documents will be sent to the e-mail address provided.

Download Brochures Amino Acid Analysis - ChromsystemsDownload Brochures Amino Acid Analysis - Chromsystems

Webinar

Web-form is not active.