Amino Acid Analysis (AAA)
The MassChrom® Amino Acid Analysis (AAA) assays in plasma/serum or urine offer a wide spectrum for your amino acid analysis. You can quantify 48 amino acids in plasma/serum. Or you opt to quantify 52 amino acids and creatinine in urine in one run. Both assays are intended, among other things, for patients with suspected metabolic diseases.
- One chromatographic platform for plasma/serum and urine
- Fast sample preparation without derivatisation
- Shorter run times than HPLC methods
- Excellent separation of all isobaric analytes
- Each analyte with its own internal standard
- Reaction vials or 96 deep well plates
Assays for Quantitative Amino Acid Analysis
MassChrom® Amino Acid Analysis in Plasma/Serum
| Order no. |
Method | Number of tests |
Characteristics |
| 75111 | LC-MS/MS | 3 x 96 | Assay with reaction vials |
| 75111/DWP | LC-MS/MS | 3 x 96 | Assay with 96 deep well plates |
Flexible Analysis: One Assay – 2 Test Modes
Benefit from the flexibility of the Chromsystems assay in plasma/serum: You can either measure 48 amino acids in 20 minutes, or alternatively determine the PKU/MSUD panel within 9 minutes - the choice is yours. The analytes measured with both, the Full Panel and the PKU/MSUD Panel, are leucine, allo-isoleucine, isoleucine, methionine, phenylalanine, tyrosine and valine. Both panels use the identical chromatographic set-up and sample preparation.
MassChrom® Amino Acid Analysis in Urine
| Order no. | Method | Number of tests | Characteristics |
| 75222 | LC-MS/MS | 3 x 96 | Assay with reaction vials |
| 75222/DWP | LC-MS/MS | 3 x 96 | Assay with 96 deep well plates |
52 Amino Acids and Creatinine in One Run
This assay allows the quantitative determination of 52 amino acids/metabolites by LC-MS/MS in 20 minutes. Creatinine is also measured within the same run, which is used for the normalisation of the urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination and subsequent dilution of the sample. The assay can be used for a number of applications, such as the determination of renal aminoacidurias, and can be ideally combined with our plasma assay without needing to change the chromatographic set-up.
All Parameters at a Glance
| Acetyltyrosine Adenosylhomocysteine Alanine β-Alanine α-Aminoadipic acid α-Aminobutyric acid β-Aminoisobutyric acid γ-Aminobutyric acid Anserine Arginine Argininosuccinic acid Asparagine Aspartic acid Carnosine |
Citrulline Homocitrulline Cystathionine Cystine Cysteine sulfate Homocystine Ethanolamine Phosphoethanolamine Glutamic acid Glutamine Glycine Histidine 1-Methylhistidine 3-Methylhistidine |
Kynurenine* Hydroxykynurenine* Leucine Allo-Isoleucine Isoleucine Lysine Hydroxylysine Methionine Ornithine Oxoproline/Pyroglutamic acid* Phenylacetylglutamine* Phenylalanine Phosphoserine Pipecolic acid |
Proline 4-Hydroxyproline Saccharopine Sarcosine Serine Taurine Threonine Tryptophan Tyrosine Valine Creatinine* |
* These 5 additional parameters can only be measured with the urine assay.
Dr Rachel Carling, Scientific Director for Viapath, reports on a study that compares the current gold standard in quantitative amino acid analysis (AAA) - ion exchange chromatography (IEC) with LC-MS/MS.
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