MassChrom® Amino Acid Analysis in Urine with Reaction Vials - LC-MS/MS

Order No.: 75222, for 3 x 96 tests
Parameters:
α-Aminoadipic Acid, α-Aminobutyric Acid, β-Alanine, β-Aminoisobutyric Acid, γ-Aminobutyric Acid, 1-Methylhistidine, 3-Methylhistidine, 4-Hydroxyproline, Acetyltyrosine, Adenosylhomocysteine, Alanine, Allo-Isoleucine, Anserine, Arginine, Argininosuccinic Acid, Asparagine, Aspartic Acid, Carnosine, Citrulline, Creatinine, Cystathionine, Cysteine S-sulfate, Cystine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocitrulline, Homocystine, Hydroxykynurenine, Hydroxylysine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Oxoproline / Pyroglutamic acid, Phenylacetylglutamine, Phenylalanine, Phosphoserine, Pipecolic Acid, Proline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Valine

Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
Creatinine normalisation within the same run
Fast sample preparation without derivatisation
Shorter run times than HPLC methods
Excellent separation of all isobaric analytes
Each analyte with its own internal standard
One chromatographic platform for plasma/serum and urine

Assay validated according to IVDR (=> Declaration of Conformity)

Chromatogram - 75222 AAA in UrineChromatogram - 75222 AAA in Urine
Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine
Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine
Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Kynurenine
Hydroxykynurenine
Leucine
Allo-Isoleucine
Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Oxoproline / Pyroglutamic acid
Phenylacetylglutamine
Phenylalanine
Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

Creatinine *

 

* With this assay the creatinine level in urine can be determined within the same run!

Clinical relevance

This assay enables the quantitative determination of 53 amino acids and metabolites in urine including the parameter creatinine, so that the results can be directly related to the creatinine value without further analysis. It is intended to be used for patients with suspected metabolic diseases in whom the urinary amino acid and metabolic product levels are of clinical importance, specifically as an aid to

  • diagnosis of cystinuria
  • diagnosis of lysinuric protein intolerance
  • differential diagnosis of Hartnup disorder versus other aminoacidurias (cystinuria and generalized aminoaciduria)
  • monitoring of treatment efficacy in previously diagnosed cystinuria patients.

 

Product advantages

  • Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
  • Creatinine normalisation within the same run
  • Fast sample preparation without derivatisation
  • Shorter run times than HPLC methods
  • Excellent separation of all isobaric analytes
  • Each analyte with its own internal standard
  • One chromatographic platform for plasma/serum and urine

 

Assay characteristics

A differentiated amino acid profile can be created in less than 20 minutes. Sample preparation consists of a fast and easy urine dilution. The method does not require derivatisation, making it robust and time-saving. Isobaric compounds are separated chromatographically. Each of the 53 analytes is safeguarded by its own internal standard compensating variations in recovery, matrix effects and ionisation effectiveness. The 3PLUS1® Multilevel Calibrator Set and MassCheck® controls are produced from synthetic urine to ensure that their composition approximates a patient sample as closely as possible. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.


Download Brochure

You need all the facts at a glance? Then you are welcome to download our brochure. After you have filled out the following form, the respective link to download your document will be sent to the e-mail address provided.

Download Brochures Amino Acid Analysis - ChromsystemsDownload Brochures Amino Acid Analysis - Chromsystems
More Information
Method of Analysis LC-MS/MS
Number of Tests 3 x 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

ULOQ for each analyte: up to double concentration of highest calibrator

For the exact values (LLOQ and ULOQ) of all analytes please refer to the instruction manual.

Specimen Urine
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 20 μl sample/calibrator/MassCheck® control
    + 50 μl Internal Standard Mix
    + 600 μl Dilution Buffer into a reaction vial.
  • Mix for 30 s (vortex) and centrifuge for 5 min at 16 000 x g.
  • Transfer 200 μl of the supernatant into an autosampler vial.
  • Inject up to 6 μl into the LC-MS/MS system.
Run Time < 20 min
Injection Volume up to 6 µl (mass spectrometer dependent)
Flow Rate 0.3–1.8 ml/min
Column Temperature + 25°C
Gradient binary gradient, flow gradient
Ionisation ESI positive
MS/MS Mode MRM
Parameters α-Aminoadipic Acid, α-Aminobutyric Acid, β-Alanine, β-Aminoisobutyric Acid, γ-Aminobutyric Acid, 1-Methylhistidine, 3-Methylhistidine, 4-Hydroxyproline, Acetyltyrosine, Adenosylhomocysteine, Alanine, Allo-Isoleucine, Anserine, Arginine, Argininosuccinic Acid, Asparagine, Aspartic Acid, Carnosine, Citrulline, Creatinine, Cystathionine, Cysteine S-sulfate, Cystine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocitrulline, Homocystine, Hydroxykynurenine, Hydroxylysine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Oxoproline / Pyroglutamic acid, Phenylacetylglutamine, Phenylalanine, Phosphoserine, Pipecolic Acid, Proline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Valine

75222 MassChrom® Amino Acid Analysis in Urine with Reaction Vials
For 3 x 96 tests

Order No. Kit components (Quantities in kit)
75001 Mobile Phase A, 950 ml (4x)
75002 Mobile Phase B, 950 ml (1x)
75009 Rinsing Solution, 500 ml (1x)
75246 Internal Standard Set, consisting of: Internal Standard Mix, 3 x 5.0 ml (lyoph.), Reconstitution Buffer, 3 x 5.5 ml
75205 Dilution Buffer, 90 ml (2x)
33006 Reaction Vials, 1.5 ml, 100 pcs. (3x)

 

Order No. Required components (not included in the kit)
75100 Analytical Column, equilibrated, with test chromatogram, 1 pc.
75228 3PLUS1® Multilevel Urine Calibrator Set Amino Acid Analysis, 4 x 0.5 ml (lyoph.)
0474 MassCheck®  Amino Acid Analysis Urine Control, Level I, 5 x 0.5 ml (lyoph.)
0475 MassCheck®  Amino Acid Analysis Urine Control, Level II, 5 x 0.5 ml (lyoph.)
0476 MassCheck®  Amino Acid Analysis Urine Control, Level III, 5 x 0.5 ml (lyoph.)
75015 Tuning Mix 1, Analytes and Internal Standards, 1 ml
75016 Tuning Mix 2, Analytes and Internal Standards, 1 ml
75017 Tuning Mix 3, Analytes and Internal Standards, 1 ml
75018 Tuning Mix 4, Analytes and Internal Standards, 1 ml
75019 Tuning Mix 5, Analytes and Internal Standards, 1 ml
75220 Tuning Mix 6, Analytes and Internal Standards, 1 ml
75212 Adjustment Reagent, for high amino acid concentrations, 30 ml

 

Order No. General lab equipment (non-CE/IVD products)
15010 PEEK Prefilter Housing, 1 pc.
15013 PEEK Prefilter, 2 µm, 5 pcs.
15080 Back Pressure Regulator Valve, 1 pc.
J0601 Autosampler Vials, screw neck, amber glass, 1.5 ml, 100 pcs.
J0410 PP Screw-on Caps, pierceable silicone/PTFE septa, 1.0 mm, 100 pcs.
J0504 PP Screw-on Caps, rubber/PTFE septa, 9 mm, 100 pcs.
J0505 Micro-Inserts for Autosampler Vials, clear glass, flat bottom, 100 pcs.
33006 Reaction Vials, 1.5 ml, 100 pcs.
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.
Chromatogram - 75222 AAA in UrineChromatogram - 75222 AAA in Urine
Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine
Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine
Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Kynurenine
Hydroxykynurenine
Leucine
Allo-Isoleucine
Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Oxoproline / Pyroglutamic acid
Phenylacetylglutamine
Phenylalanine
Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

Creatinine *

 

* With this assay the creatinine level in urine can be determined within the same run!

Clinical relevance

This assay enables the quantitative determination of 53 amino acids and metabolites in urine including the parameter creatinine, so that the results can be directly related to the creatinine value without further analysis. It is intended to be used for patients with suspected metabolic diseases in whom the urinary amino acid and metabolic product levels are of clinical importance, specifically as an aid to

  • diagnosis of cystinuria
  • diagnosis of lysinuric protein intolerance
  • differential diagnosis of Hartnup disorder versus other aminoacidurias (cystinuria and generalized aminoaciduria)
  • monitoring of treatment efficacy in previously diagnosed cystinuria patients.

 

Product advantages

  • Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
  • Creatinine normalisation within the same run
  • Fast sample preparation without derivatisation
  • Shorter run times than HPLC methods
  • Excellent separation of all isobaric analytes
  • Each analyte with its own internal standard
  • One chromatographic platform for plasma/serum and urine

 

Assay characteristics

A differentiated amino acid profile can be created in less than 20 minutes. Sample preparation consists of a fast and easy urine dilution. The method does not require derivatisation, making it robust and time-saving. Isobaric compounds are separated chromatographically. Each of the 53 analytes is safeguarded by its own internal standard compensating variations in recovery, matrix effects and ionisation effectiveness. The 3PLUS1® Multilevel Calibrator Set and MassCheck® controls are produced from synthetic urine to ensure that their composition approximates a patient sample as closely as possible. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.


Download Brochure

You need all the facts at a glance? Then you are welcome to download our brochure. After you have filled out the following form, the respective link to download your document will be sent to the e-mail address provided.

Download Brochures Amino Acid Analysis - ChromsystemsDownload Brochures Amino Acid Analysis - Chromsystems
More Information
Method of Analysis LC-MS/MS
Number of Tests 3 x 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

ULOQ for each analyte: up to double concentration of highest calibrator

For the exact values (LLOQ and ULOQ) of all analytes please refer to the instruction manual.

Specimen Urine
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 20 μl sample/calibrator/MassCheck® control
    + 50 μl Internal Standard Mix
    + 600 μl Dilution Buffer into a reaction vial.
  • Mix for 30 s (vortex) and centrifuge for 5 min at 16 000 x g.
  • Transfer 200 μl of the supernatant into an autosampler vial.
  • Inject up to 6 μl into the LC-MS/MS system.
Run Time < 20 min
Injection Volume up to 6 µl (mass spectrometer dependent)
Flow Rate 0.3–1.8 ml/min
Column Temperature + 25°C
Gradient binary gradient, flow gradient
Ionisation ESI positive
MS/MS Mode MRM
Parameters α-Aminoadipic Acid, α-Aminobutyric Acid, β-Alanine, β-Aminoisobutyric Acid, γ-Aminobutyric Acid, 1-Methylhistidine, 3-Methylhistidine, 4-Hydroxyproline, Acetyltyrosine, Adenosylhomocysteine, Alanine, Allo-Isoleucine, Anserine, Arginine, Argininosuccinic Acid, Asparagine, Aspartic Acid, Carnosine, Citrulline, Creatinine, Cystathionine, Cysteine S-sulfate, Cystine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocitrulline, Homocystine, Hydroxykynurenine, Hydroxylysine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Oxoproline / Pyroglutamic acid, Phenylacetylglutamine, Phenylalanine, Phosphoserine, Pipecolic Acid, Proline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Valine

75222 MassChrom® Amino Acid Analysis in Urine with Reaction Vials
For 3 x 96 tests

Order No. Kit components (Quantities in kit)
75001 Mobile Phase A, 950 ml (4x)
75002 Mobile Phase B, 950 ml (1x)
75009 Rinsing Solution, 500 ml (1x)
75246 Internal Standard Set, consisting of: Internal Standard Mix, 3 x 5.0 ml (lyoph.), Reconstitution Buffer, 3 x 5.5 ml
75205 Dilution Buffer, 90 ml (2x)
33006 Reaction Vials, 1.5 ml, 100 pcs. (3x)

 

Order No. Required components (not included in the kit)
75100 Analytical Column, equilibrated, with test chromatogram, 1 pc.
75228 3PLUS1® Multilevel Urine Calibrator Set Amino Acid Analysis, 4 x 0.5 ml (lyoph.)
0474 MassCheck®  Amino Acid Analysis Urine Control, Level I, 5 x 0.5 ml (lyoph.)
0475 MassCheck®  Amino Acid Analysis Urine Control, Level II, 5 x 0.5 ml (lyoph.)
0476 MassCheck®  Amino Acid Analysis Urine Control, Level III, 5 x 0.5 ml (lyoph.)
75015 Tuning Mix 1, Analytes and Internal Standards, 1 ml
75016 Tuning Mix 2, Analytes and Internal Standards, 1 ml
75017 Tuning Mix 3, Analytes and Internal Standards, 1 ml
75018 Tuning Mix 4, Analytes and Internal Standards, 1 ml
75019 Tuning Mix 5, Analytes and Internal Standards, 1 ml
75220 Tuning Mix 6, Analytes and Internal Standards, 1 ml
75212 Adjustment Reagent, for high amino acid concentrations, 30 ml

 

Order No. General lab equipment (non-CE/IVD products)
15010 PEEK Prefilter Housing, 1 pc.
15013 PEEK Prefilter, 2 µm, 5 pcs.
15080 Back Pressure Regulator Valve, 1 pc.
J0601 Autosampler Vials, screw neck, amber glass, 1.5 ml, 100 pcs.
J0410 PP Screw-on Caps, pierceable silicone/PTFE septa, 1.0 mm, 100 pcs.
J0504 PP Screw-on Caps, rubber/PTFE septa, 9 mm, 100 pcs.
J0505 Micro-Inserts for Autosampler Vials, clear glass, flat bottom, 100 pcs.
33006 Reaction Vials, 1.5 ml, 100 pcs.
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.