

Vitamin Profiling
Choose from a range of CE-IVD validated and complete assays for vitamin profiling. Calibrators and controls are also available separately.
- HPLC/UHPLC and LC-MS/MS assays available
- Reliable methods with fast sample preparation
- Calibrators and controls available separately
Assays Available for Vitamin Profiling
Assays for the Combined Analysis of Vitamin A and E in Serum/Plasma
Order No. | Method | Number of tests | Analysis time | Characteristics |
34000 | HPLC | 100 | 9 min | Standard method |
34000/Premix | HPLC | 100 | 9 min | One-step sample prep / pre-mixed tubes |
34900/UHPLC | UHPLC | 1.000 | 3.5 min | One-step sample prep / pre-mixed tubes Short analysis time, high sample throughput |
34700/F | HPLC | 5 x 96 | 9 min | HPLC method, automated |
34900/F | UHPLC | 10 x 96 | 3.5 min | UHPLC method, automated. Short analysis time, high sample throughput |
Vitamin A (retinol) is essential for the formation of rhodopsin, for bone metabolism, and for the synthesis of steroid hormones. Deficiency of vitamin A leads to night blindness, dry skin, and loss of hair. Vitamin E (α-tocopherol) is a potent antioxidant that protects LDL cholesterol and cellular membranes from lipid peroxidation, which mainly occurs as a result of increased oxidative stress in the organism.
Chromsystems offers CE-IVD validated complete assays as well as calibrators and controls. Plus there are solutions available for any requirements:
- Standard method or one-step sample preparation with Pre-mixed Tubes
- UHPLC methods for short analysis time and high sample throughput
- Automated methods
Assays for the Combined Analysis of Vitamin B1 and B6 in Whole Blood and Plasma/Serum
Order No. | Method | Specimen | Number of tests | Analysis time | Characteristics |
87000 | LC-MS/MS | Whole Blood | 480 | 2.5 min | Without derivatisation, quick sample prep, high sample throughput |
87000/DWP | LC-MS/MS | Whole Blood | 480 | 2.5 min | With 96 deep well plates, quick sample prep, high sample throughput |
52052 | HPLC | Whole Blood B6 also Plasma |
100 | < 9 min | Classic method |
52052/Premix | HPLC | Whole Blood B6 also Plasma |
100 | < 9 min | Simplified method with pre-mixed tubes |
52952/UHPLC | UHPLC | Whole Blood B6 also Plasma |
1000 | < 4 min | Simplified method with pre-mixed tubes, high sample throughput |
52752/F | HPLC | Whole Blood | 960 | < 9 min | Classic method, automated |
52952/UHPLC/F |
UHPLC | Whold Blood | 960 | 4 min | UHPLC method, automated |
Assay for the Single Analysis of Vitamin B1 in Whole Blood
Order No. | Method | Specimen | Number of tests | Analysis time | Characteristics |
35000 | HPLC | Whole Blood | 100 | < 6 min | No post-column derivatisation needed |
Assays for the Single Analysis of Vitamin B6 in Whole Blood and Plasma/Serum
Order No. | Method | Specimen | Number of tests | Analysis time | Characteristics |
31000/S | HPLC | Plasma/Serum | 100 | < 8 min | Fast and easy sample prep |
31000/WB |
HPLC | Whole Blood | 100 | < 8 min | Fast and easy sample prep |
LC-MS/MS Solution
Our mass spectrometry assay allows the determination of the active forms of vitamin B1 (TPP) and vitamin B6 (PLP) in whole blood by LC-MS/MS. The sample preparation can be performed very quickly and consists of a simple protein precipitation eliminating the need for a derivatisation – ideal for laboratories with higher throughputs.
You will find an overview with further details in our LC-MS/MS brochure to download.
HPLC/UHPLC Solutions
Various methods for any requirements:
- Standard method or with Pre-mixed Tubes, for simplified manual sample preparation
- UHPLC methods for short analysis time and high sample throughput
- Automated methods
For a detailed overview please download the HPLC/UHPLC brochure.
Assay for the Analysis of Vitamin B2 in Whole Blood
Order No. | Method | Number of tests | Analysis time | Characteristics |
37000 | HPLC | 100 | < 9 min | Simultaneous determination of riboflavin, FMN and FAD |
Riboflavin (Vitamin B2) is the precursor of two coenzymes known as flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Both are essential for tissue respiration and the generation of energy from the metabolism of carbohydrates, amino acids and fats. Riboflavin is vital for normal reproduction, growth, repair and development of body tissues including the skin, hair, nails, connective tissue and immune system. Riboflavin is mainly converted into FMN and FAD in the small intestine, liver, heart and kidney.
Assays for the Analysis of Vitamin C in Plasma/Serum
Order No. | Method | Number of tests | Analysis time | Characteristics |
65065 | HPLC | 100 | 5 min | Standard method |
65765/F | HPLC | 480 | 5 min | Automated method with 96 well filter plates |
Vitamin C (ascorbic acid) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.
Assays for the Analysis of Vitamin D3/D2 in Plasma/Serum
Order No. | Method | Number of tests | Analysis time | Characteristics |
38038 | HPLC | 100 | 10 min | Standard method |
38900/1000 |
HPLC | 1000 | 10 min | Automated method |
62000 62000/1000 |
LC-MS/MS | 200 1000 |
5 min | With online sample preparation |
62000/1000/F | LC-MS/MS | 1000 | 5 min | Automated method with 96 well filter plates |
Epimer Upgrade for 62000 | LC-MS/MS | 200 | Simultaneous measurement of vitamin D3, vitamin D2 and its C3-epimers |
Vitamin D is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.
Assay for the Analysis of β-Carotene in Serum/Plasma
Order No. | Method | Number of tests | Analysis time | Characteristics |
32000 | HPLC | 100 | < 10 min | Fast and easy sample prep |
β-Carotene (Provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products. In humans, all-trans-β-carotene is functionally and quantitatively the most important of about 20 carotenoids present in the blood plasma. This assay assures reliable separation of α-carotene, cis-β-carotene and all-trans-β-carotene as well as the carotenoids cryptoxanthin and lycopene.
Assay for the Analysis of Methylmalonic Acid in Serum/Plasma and Urine
Order No. | Method | Number of tests | Analysis time | Characteristics |
64000 | LC-MS/MS | 400 | 3.5 min | Clean-up tubes for fast processing of serum/plasma samples or direct injection of urine samples after dilution |
64000 Urine Upgrade Set | LC-MS/MS | 200 | 3.5 min | Upgrade set for methylmalonic acid in urine |
Methylmalonic acid (MMA) is an intermediate of amino acid metabolism which results from the conversion of propionyl-coenzyme A to succinyl-coenzyme A in a vitamin B12-dependent reaction, and is regarded as an early, sensitive biomarker of vitamin B12-deficiency. Vitamin B12-insufficiency leads to high concentrations of MMA in plasma, serum and urine. Our MassChrom® Methylmalonic Acid (MMA) kits allow the specific determination of methylmalonic acid in all three matrices.
Download Brochures
Vitamins A & E in Serum/Plasma by HPLC and UHPLC


Vitamins B1 & B6 LC-MS/MS Solution


Vitamins B1 & B6 HPLC/UHPLC Solutions

