Vitamin Profiling

Vitamin A (retinol) is essential for the formation of rhodopsin, for bone metabolism, and for the synthesis of steroid hormones. Deficiency of vitamin A leads to night blindness, dry skin, and loss of hair. Vitamin E (α-tocopherol) is a potent antioxidant that protects LDL cholesterol and cellular membranes from lipid peroxidation, which mainly occurs as a result of increased oxidative stress in the organism.

Chromsystems offers CE-IVD validated complete assays as well as calibrators and controls. Plus there are solutions available for any requirements:

•    Standard method or one-step sample preparation with Pre-mixed Tubes
•    UHPLC methods for short analysis time and high sample throughput
•    Automated methods

Do you need all the facts at a glance? Then feel free to download our brochure vitamins A and E in serum/plasma on the brochure download page.

LC-MS/MS Solution

Our mass spectrometry assay allows the determination of the active forms of vitamin B1 (TPP) and vitamin B6 (PLP) in whole blood by LC-MS/MS. The sample preparation can be performed very quickly and consists of a simple protein precipitation eliminating the need for a derivatisation – ideal for laboratories with higher throughputs.

HPLC/UHPLC Solutions

Various methods for any requirements:

• Standard method or with Pre-mixed Tubes, for simplified manual sample preparation
• UHPLC methods for short analysis time and high sample throughput
• Automated methods

A good overview of all methods can be found in our brochures vitamins B1 and B6 with LC-MS/MS and vitamins B1 and B6 with HPLC and UHPLC, which you can download from the brochure download page.

Riboflavin (Vitamin B2) is the precursor of two coenzymes known as flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Both are essential for tissue respiration and the generation of energy from the metabolism of carbohydrates, amino acids and fats. Riboflavin is vital for normal reproduction, growth, repair and development of body tissues including the skin, hair, nails, connective tissue and immune system. Riboflavin is mainly converted into FMN and FAD in the small intestine, liver, heart and kidney.

Vitamin C (ascorbic acid) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

Vitamin D is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.

β-Carotene (Provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products. In humans, all-trans-β-carotene is functionally and quantitatively the most important of about 20 carotenoids present in the blood plasma. This assay assures reliable separation of α-carotene, cis-β-carotene and all-trans-β-carotene as well as the carotenoids cryptoxanthin and lycopene.

Methylmalonic acid (MMA) is an intermediate of amino acid metabolism which results from the conversion of propionyl-coenzyme A to succinyl-coenzyme A in a vitamin B12-dependent reaction, and is regarded as an early, sensitive biomarker of vitamin B12-deficiency. Vitamin B12-insufficiency leads to high concentrations of MMA in plasma, serum and urine. Our MassChrom^® Methylmalonic Acid (MMA) kits allow the specific determination of methylmalonic acid in all three matrices.