Vitamin Profiling

Choose from a range of CE-IVD validated and complete assays for vitamin profiling. Calibrators and controls are also available separately. 

  • HPLC/UHPLC and LC-MS/MS assays available
  • Reliable methods with fast sample preparation
  • Calibrators and controls available separately 

Assays Available for Vitamin Profiling

Assays for the Combined Analysis of Vitamin A and E in Serum/Plasma

Order No. Method Number of tests Analysis time Characteristics
34000 HPLC 100 9 min Standard method
34000/Premix HPLC 100 9 min One-step sample prep / pre-mixed tubes
34900/UHPLC UHPLC 1.000 3.5 min One-step sample prep / pre-mixed tubes
Short analysis time, high sample throughput
34700/F HPLC 5 x 96 9 min HPLC method, automated
34900/F UHPLC 10 x 96 3.5 min UHPLC method, automated. Short analysis time, high sample throughput

Vitamin A (retinol) is essential for the formation of rhodopsin, for bone metabolism, and for the synthesis of steroid hormones. Deficiency of vitamin A leads to night blindness, dry skin, and loss of hair. Vitamin E (α-tocopherol) is a potent antioxidant that protects LDL cholesterol and cellular membranes from lipid peroxidation, which mainly occurs as a result of increased oxidative stress in the organism.

Chromsystems offers CE-IVD validated complete assays as well as calibrators and controls. Plus there are solutions available for any requirements:

  •    Standard method or one-step sample preparation with Pre-mixed Tubes
  •    UHPLC methods for short analysis time and high sample throughput
  •    Automated methods

Assays for the Combined Analysis of Vitamin B1 and B6 in Whole Blood and Plasma/Serum

Order No. Method  Specimen Number of tests Analysis time Characteristics
87000 LC-MS/MS Whole Blood 480 2.5 min Without derivatisation, quick sample prep,
high sample throughput
87000/DWP LC-MS/MS Whole Blood 480 2.5 min With 96 deep well plates, quick sample prep,
high sample throughput
52052 HPLC Whole Blood
B6 also Plasma
100 < 9 min Classic method
52052/Premix HPLC Whole Blood
B6 also Plasma
100 < 9 min Simplified method with pre-mixed tubes
52952/UHPLC UHPLC Whole Blood
B6 also Plasma
1000 < 4 min Simplified method with pre-mixed tubes,
high sample throughput
52752/F HPLC Whole Blood 960 < 9 min Classic method, automated
52952/UHPLC/F
UHPLC Whold Blood 960 4 min UHPLC method, automated

Assay for the Single Analysis of Vitamin B1 in Whole Blood

Order No. Method  Specimen Number of tests Analysis time Characteristics
35000 HPLC Whole Blood 100  < 6 min No post-column derivatisation needed

Assays for the Single Analysis of Vitamin B6 in Whole Blood and Plasma/Serum

Order No. Method  Specimen Number of tests Analysis time Characteristics
31000/S HPLC Plasma/Serum 100 < 8 min Fast and easy sample prep
31000/WB
HPLC Whole Blood 100 < 8 min Fast and easy sample prep

LC-MS/MS Solution

Our mass spectrometry assay allows the determination of the active forms of vitamin B1 (TPP) and vitamin B6 (PLP) in whole blood by LC-MS/MS. The sample preparation can be performed very quickly and consists of a simple protein precipitation eliminating the need for a derivatisation – ideal for laboratories with higher throughputs.

You will find an overview with further details in our LC-MS/MS brochure to download.

 

HPLC/UHPLC Solutions

Various methods for any requirements:

  • Standard method or with Pre-mixed Tubes, for simplified manual sample preparation
  • UHPLC methods for short analysis time and high sample throughput
  • Automated methods

For a detailed overview please download the HPLC/UHPLC brochure.

Assay for the Analysis of Vitamin B2 in Whole Blood

Order No. Method Number of tests Analysis time Characteristics
37000 HPLC 100  < 9 min Simultaneous determination of riboflavin, FMN and FAD

Riboflavin (Vitamin B2) is the precursor of two coenzymes known as flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Both are essential for tissue respiration and the generation of energy from the metabolism of carbohydrates, amino acids and fats. Riboflavin is vital for normal reproduction, growth, repair and development of body tissues including the skin, hair, nails, connective tissue and immune system. Riboflavin is mainly converted into FMN and FAD in the small intestine, liver, heart and kidney.

Assays for the Analysis of Vitamin C in Plasma/Serum

Order No. Method Number of tests Analysis time Characteristics
65065 HPLC 100 5 min Standard method
65765/F HPLC 480 5 min Automated method with 96 well filter plates

Vitamin C (ascorbic acid) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

Assays for the Analysis of Vitamin D3/D2 in Plasma/Serum

Order No. Method Number of tests Analysis time Characteristics
38038 HPLC 100 10 min Standard method
38900/1000
HPLC 1000 10 min Automated method
62000
62000/1000
LC-MS/MS 200
1000
5 min With online sample preparation
62000/1000/F LC-MS/MS 1000 5 min Automated method with 96 well filter plates
Epimer Upgrade for 62000 LC-MS/MS 200   Simultaneous measurement of vitamin D3, vitamin D2 and its C3-epimers

Vitamin D is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.

Assay for the Analysis of β-Carotene in Serum/Plasma

Order No. Method Number of tests Analysis time Characteristics
32000 HPLC 100  < 10 min Fast and easy sample prep

β-Carotene (Provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products. In humans, all-trans-β-carotene is functionally and quantitatively the most important of about 20 carotenoids present in the blood plasma. This assay assures reliable separation of α-carotene, cis-β-carotene and all-trans-β-carotene as well as the carotenoids cryptoxanthin and lycopene.

Assay for the Analysis of Methylmalonic Acid in Serum/Plasma and Urine

Order No. Method Number of tests Analysis time Characteristics
64000 LC-MS/MS 400 3.5 min Clean-up tubes for fast processing of serum/plasma samples or
direct injection of urine samples after dilution
64000 Urine Upgrade Set LC-MS/MS 200 3.5 min Upgrade set for methylmalonic acid in urine

Methylmalonic acid (MMA) is an intermediate of amino acid metabolism which results from the conversion of propionyl-coenzyme A to succinyl-coenzyme A in a vitamin B12-dependent reaction, and is regarded as an early, sensitive biomarker of vitamin B12-deficiency. Vitamin B12-insufficiency leads to high concentrations of MMA in plasma, serum and urine. Our MassChrom® Methylmalonic Acid (MMA) kits allow the specific determination of methylmalonic acid in all three matrices.

 

Download Brochures

You need all the facts at a glance? Then you are welcome to download our brochure. After you have filled out the following form, the respective link to download your document will be sent to the e-mail address provided.


Vitamins A & E in Serum/Plasma by HPLC and UHPLC


Vitamins B1 & B6 LC-MS/MS Solution


Vitamins B1 & B6 HPLC/UHPLC Solutions