Vitamins A and E in Serum/Plasma - HPLC Basic Kit
Short run time of less than 3 minutes
Optimized precipitation
Choice to use HPLC column at ambient temperature or at 35 °C
Assay validated according to IVDR (=> Declaration of Conformity)
Column temperature: 35 °C
Vitamin A (Retinol)
Vitamin E (α-Tocopherol)
Clinical relevance
This assay enables the simultaneous quantitative determination of vitamin A (retinol) and vitamin E (α-tocopherol) in human serum or plasma samples by HPLC.
It is intended to be used for determination of vitamin A and vitamin E levels where indicated
• for screening in patients with suspected vitamin A and/or E deficiency,
• for screening in patients with suspected vitamin A and/or E excess,
• for monitoring of patients under vitamin A and/or E supplementation.
Product advantages
• Short run time of less than 3 minutes
• Optimized precipitation
• Choice to use HPLC column at ambient temperature or at 35 °C
• Easy sample preparation
• No cooling of eluates – no cooled autosampler necessary
• No precolumn required
• No UHPLC needed: Short run time even with HPLC! – Also applicable to UHPLC systems
Assay characteristics
The fast and easy sample preparation can be performed with reaction vials or 96 deep well plates. The analytes are released from their transport protein or lipoproteins by precipitation. Subsequently, the substances are chromatographically separated in one isocratic HPLC run and quantified using a UV detector.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Two types of kits available
Basic Kit
• Only sample preparation reagents and plates/seals included
• No excess quantities of mobile phase and calibrators
|
34400-DWP-BK Basic Kit |
|
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 34456 | 96 Deep Well Plates, 6 pcs. (1x) |
| 34459 | Cross-slitted Adhesive Seals for 96 Well Plates, 3 pcs. (1x) |
Reagent Kit
• Mobile phase and calibrator included
|
34400-DWP Reagent Kit |
|
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 34456 | 96 Deep Well Plates, 6 pcs. (1x) |
| 34459 | Cross-slitted Adhesive Seals for 96 Well Plates, 3 pcs. (1x) |
| 34001 | Mobile Phase, 1000 ml (1x) |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) (1x) |
| Method of Analysis | HPLC |
|---|---|
| Sample Preparations | 200 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Serum: Plasma: Different systems might show different performance data. |
| Specimen | Serum/Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Sample preparation with 96 deep well plates:
For the sample preparation with reaction vials, please refer to the instruction manual. |
| Run Time | depending on column temperature: 2.9 min (35 °C) or 3.7 min (ambient) |
| Injection Volume | 5 – 20 µl |
| Flow Rate | 0.8 ml/min |
| Wavelengths | 325 nm, |
| Additional Info | For the analysis any isocratic HPLC system with UV detector is suitable. For simultaneous analysis of retinol and α-tocopherol, a detector with programmable wavelength switching is very useful as these analytes are detected at different wavelengths. |
| Parameters | Vitamin A (Retinol), Vitamin E (α-Tocopherol) |
Vitamins A and E in Serum/Plasma - HPLC Basic Kit (34400-DWP-BK)
For 200 sample preparations
| Order No. | Basic Kit components (Quantities in kit) |
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 34456 | 96 Deep Well Plates, 6 pcs. (1x) |
| 34459 | Cross-slitted Adhesive Seals for 96 Well Plates, 3 pcs. (1x) |
| Order No. | Required components (not included in the Basic Kit) |
| 34300 | HPLC Column, equilibrated, with test chromatogram, 1 pc. |
| 34001 | Mobile Phase, 1000 ml |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) |
| 0036 | Serum Control, Level I, 5 x 2 ml (lyoph.) |
| 0037 | Serum Control, Level II, 5 x 2 ml (lyoph.) |
| 15012 | PEEK-encased Prefilter, 0.5 µm, 1 pc. |
34760 |
Optional, as an alternative to 34459 (Cross-slitted Adhesive Seals): Pierceable Heat Seals for 96 Well Plates, 6 pcs. |
| Order No. | General lab equipment (non-CE/IVD products) |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
42740 |
When using Pierceable Heat Seals (34760): Chromsystems Heat Sealer, 1 pc. |
-
Cross-slitted Adhesive Seals for 96 Well PlatesOrder no.: 34459Vitamins A and E in Serum/Plasma – HPLC
-
HPLC Column Vitamins A and E in Serum/PlasmaOrder no.: 34300Vitamins A and E in Serum/Plasma – HPLC -
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC -
Pierceable Heat Seals for 96 Well PlatesOrder no.: 34760Optional, as an alternative to 34459 (Cross-slitted Adhesive Seals)
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
-
HPLC Column Vitamins A and E in Serum/PlasmaOrder no.: 34300Vitamins A and E in Serum/Plasma – HPLC
-
Chromsystems Heat SealerOrder no.: 42740Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint
Column temperature: 35 °C
Vitamin A (Retinol)
Vitamin E (α-Tocopherol)
Clinical relevance
This assay enables the simultaneous quantitative determination of vitamin A (retinol) and vitamin E (α-tocopherol) in human serum or plasma samples by HPLC.
It is intended to be used for determination of vitamin A and vitamin E levels where indicated
• for screening in patients with suspected vitamin A and/or E deficiency,
• for screening in patients with suspected vitamin A and/or E excess,
• for monitoring of patients under vitamin A and/or E supplementation.
Product advantages
• Short run time of less than 3 minutes
• Optimized precipitation
• Choice to use HPLC column at ambient temperature or at 35 °C
• Easy sample preparation
• No cooling of eluates – no cooled autosampler necessary
• No precolumn required
• No UHPLC needed: Short run time even with HPLC! – Also applicable to UHPLC systems
Assay characteristics
The fast and easy sample preparation can be performed with reaction vials or 96 deep well plates. The analytes are released from their transport protein or lipoproteins by precipitation. Subsequently, the substances are chromatographically separated in one isocratic HPLC run and quantified using a UV detector.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Two types of kits available
Basic Kit
• Only sample preparation reagents and plates/seals included
• No excess quantities of mobile phase and calibrators
|
34400-DWP-BK Basic Kit |
|
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 34456 | 96 Deep Well Plates, 6 pcs. (1x) |
| 34459 | Cross-slitted Adhesive Seals for 96 Well Plates, 3 pcs. (1x) |
Reagent Kit
• Mobile phase and calibrator included
|
34400-DWP Reagent Kit |
|
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 34456 | 96 Deep Well Plates, 6 pcs. (1x) |
| 34459 | Cross-slitted Adhesive Seals for 96 Well Plates, 3 pcs. (1x) |
| 34001 | Mobile Phase, 1000 ml (1x) |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) (1x) |
| Method of Analysis | HPLC |
|---|---|
| Sample Preparations | 200 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Serum: Plasma: Different systems might show different performance data. |
| Specimen | Serum/Plasma |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Sample preparation with 96 deep well plates:
For the sample preparation with reaction vials, please refer to the instruction manual. |
| Run Time | depending on column temperature: 2.9 min (35 °C) or 3.7 min (ambient) |
| Injection Volume | 5 – 20 µl |
| Flow Rate | 0.8 ml/min |
| Wavelengths | 325 nm, |
| Additional Info | For the analysis any isocratic HPLC system with UV detector is suitable. For simultaneous analysis of retinol and α-tocopherol, a detector with programmable wavelength switching is very useful as these analytes are detected at different wavelengths. |
| Parameters | Vitamin A (Retinol), Vitamin E (α-Tocopherol) |
Vitamins A and E in Serum/Plasma - HPLC Basic Kit (34400-DWP-BK)
For 200 sample preparations
| Order No. | Basic Kit components (Quantities in kit) |
| 34404 | Internal Standard, 40 ml (1x) |
| 34405 | Precipitation Reagent, 40 ml (1x) |
| 34456 | 96 Deep Well Plates, 6 pcs. (1x) |
| 34459 | Cross-slitted Adhesive Seals for 96 Well Plates, 3 pcs. (1x) |
| Order No. | Required components (not included in the Basic Kit) |
| 34300 | HPLC Column, equilibrated, with test chromatogram, 1 pc. |
| 34001 | Mobile Phase, 1000 ml |
| 34004 | Serum Calibration Standard, 5 x 1 ml (lyoph.) |
| 0036 | Serum Control, Level I, 5 x 2 ml (lyoph.) |
| 0037 | Serum Control, Level II, 5 x 2 ml (lyoph.) |
| 15012 | PEEK-encased Prefilter, 0.5 µm, 1 pc. |
34760 |
Optional, as an alternative to 34459 (Cross-slitted Adhesive Seals): Pierceable Heat Seals for 96 Well Plates, 6 pcs. |
| Order No. | General lab equipment (non-CE/IVD products) |
| 15070 | Stainless Steel Prefilter Housing, 1 pc. |
42740 |
When using Pierceable Heat Seals (34760): Chromsystems Heat Sealer, 1 pc. |
-
Cross-slitted Adhesive Seals for 96 Well PlatesOrder no.: 34459Vitamins A and E in Serum/Plasma – HPLC
-
HPLC Column Vitamins A and E in Serum/PlasmaOrder no.: 34300Vitamins A and E in Serum/Plasma – HPLC
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
-
Pierceable Heat Seals for 96 Well PlatesOrder no.: 34760Optional, as an alternative to 34459 (Cross-slitted Adhesive Seals)
-
Vitamins A and E Serum Calibration StandardOrder no.: 34004Vitamins A and E in Serum/Plasma- HPLC/UHPLC
-
HPLC Column Vitamins A and E in Serum/PlasmaOrder no.: 34300Vitamins A and E in Serum/Plasma – HPLC
-
Chromsystems Heat SealerOrder no.: 42740
Easy sealing of 96 well plates
Adjustable time and temperature
Ergonomic lever
Compatible with a wide range of microplates
Fast startup
Small footprint