Antiepileptic Drugs in Serum/Plasma, FAST ELUTION - HPLC
Analysis of all relevant antiepileptic drugs in a single run
Fast sample preparation: only one precipitation step
Alternatively fast elution (< 5 min) or high resolution chromatographic separation
Metabolite analysis included
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation
Epileptic seizures are the result of synchronous discharges of neuron groups in the brain that can lead to sudden and involuntary stereotypical behavioural or sensory disorders. There are numerous types of seizures, each of which requires specialised therapy.
Therapy with anticonvulsive medication (antiepileptics) leads to a reduction in seizures and sometimes even a complete elimination of seizures in most treated patients. The condition is usually patient compliance, e.g. regular use of the medication. Therefore, monitoring of blood levels is essential, especially when starting the use of the medication, as well as for further modification for different life phases. For example, children usually metabolise medication faster than teenagers or adults.
- Analysis of all major antiepileptics in one run
- Fast sample preparation with only one precipitation step
- Options for fast elution (< 5 minutes) or high resolution chromatography
- Measurement of metabolites is possible
This Chromsystems AED kit enables the determination of the antiepileptics carbamazepine, ethosuximide, lamotrigin, phenobarbital, phenytoin, primidone, sulthiame, oxcarbazepine (Trileptal®) and zonisamide as well as of the metabolites 10-OH-carbamazepine and carbamazepine-10,11-epoxide in serum/plasma using HPLC. Sample preparation is based on a simple and very efficient precipitation of plasma proteins. Fast chromatography (Fast Elution < 5 minutes) and high resolution chromatography (High Resolution) are available as options for quantifying the analytes.
These analytes as well as other AEDs can also be measured with LC-MS/MS using our Parameter Set for Antiepileptic Drugs from the MassTox® TDM Series A.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Linearity||up to 250 mg/l|
CV < 4 %
CV < 6 %
|Analysis Time||Fast Elution: < 5 mim; High Resolution: < 22 min|
|Sample Stability||Stored at +2 to +8 °C for up to 1 week. For longer storage (up to 2 weeks) deep-freeze below -18 °C.|
|Injection Volume||20 µl|
|Flow rate||Fast Elution: 2.0 ml/min; High Resolution: 1.2 ml/min|
|Column temperature||ambient (~25 °C)|
|Additional Info||Isocratic HPLC system with UV detector.|
A precolumn has to be used.
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Parameter||10-OH-Carbamazepine, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Lamotrigine, Oxcarbamazepine (Trileptal®), Phenobarbital, Phenytoin, Primidone, Sultiame, Zonisamide|