Vitamin B1 in Whole Blood - HPLC
No post-column derivatisation
Highly stable calibration standards and controls
Vitamin B1 (thiamine) plays an important role in deriving energy from carbohydrates and fats. The active form is thiamine pyrophosphate (TPP), a coenzyme for some enzymatic reactions in the carbohydrate metabolism and for the pyruvate decarboxylase. TPP is also significantly involved in the oxidative degradation of glucose. The determination of TPP is more significant than measurements of total thiamine, as by this method only the physiologically active form is taken into account.
Thiamine need is closely associated with caloric intake due to its central role in the energy metabolism: the more energy is needed, e.g. for competitive sports, the more thiamine the food must contain. It also prevents the accumulation of toxic by-products of cell metabolism that may damage the heart or nervous system.
- No post-column derivatisation
- Simple handling
- Stable standards and controls
TPP can be determined fast and simply direct from whole blood using this Chromsystems kit. The kit is optimised for isocratic HPLC systems with fluorescence detector. The derivatisation necessary for fluorescence detection is included in the sample preparation procedure, and there is no need for instrument-intensive post-column derivatisation for the fluorescence detection. A special HPLC column and an optimised mobile phase allow precise and reliable quantification in less than 6 minutes.
|Number of Tests||100|
|Limit of quantification||0.8 μg/l|
|Linearity||up to at least 750 μg/l|
CV = 2.8–4.9 %
CV = 4.3–4.7 %
|Analysis Time||6 min|
|Sample Stability||Prepared samples are stable at +2 to +8 °C for at least 3 days.|
The patient samples are stable at +2 to +8 °C for 24 hours, light-protected in a closed vial. For longer storage (up to 6 months) keep samples below -18 °C.
|Injection Volume||50 µl|
|Flow rate||1 ml/min|
|Column temperature||ambient (~ 25 °C)|
EX 367 nm
EM 435 nm
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Method of Analysis||HPLC|
|Parameter||Vitamin B1 (TPP, thiamine pyrophosphate)|
Every isocratic HPLC system with fluorescence detector is suitable.