Vitamin B1 in Whole Blood - HPLC Basic Kit
Vitamin B1 (TPP, thiamine pyrophosphate)
Clinical relevance
This assay allows the quantitative determination of the physiologically active form of vitamin B1, thiamine pyrophosphate, in human whole blood samples by HPLC.
It is intended to be used for determination of vitamin B1 levels where indicated
• in patients with suspected vitamin B1 deficiency,
• in patients with suspected vitamin B1 excess,
• in patients under vitamin B1 supplementation therapy.
Assay characteristics
This assay requires an isocratic HPLC system with fluorescence detector. The usual post-column derivatisation using extensive equipment is replaced by a simple pre-column derivatisation. Sample preparation is restricted to a single precipitation step after addition of the extraction buffer and subsequent derivatisation in a reaction vial.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Two types of kits available
Basic Kit
• Only sample preparation reagents and vials included
• No excess quantities of mobile phase and calibrators
|
35000-BK Basic Kit |
|
| 37003 | Extraction Buffer, 10 ml (1x) |
| 37004 | Precipitation Reagent, 30 ml (1x) |
| 35005 | Derivatisation Reagent 1, 5 x 0.3 ml (1x) |
| 35006 | Derivatisation Reagent 2, 20 ml (1x) |
| 35007 | Stabilisation Buffer, 10 ml (1x) |
| 35009 | Neutralisation Buffer, 10 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x) |
Reagent Kit
• Mobile phase and calibrator included
|
35000 Reagent Kit |
|
| 37003 | Extraction Buffer, 10 ml (1x) |
| 37004 | Precipitation Reagent, 30 ml (1x) |
| 35005 | Derivatisation Reagent 1, 5 x 0.3 ml (1x) |
| 35006 | Derivatisation Reagent 2, 20 ml (1x) |
| 35007 | Stabilisation Buffer, 10 ml (1x) |
| 35009 | Neutralisation Buffer, 10 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x) |
| 35021 | Mobile Phase, 1000 ml, (1x) |
| 37008 | Whole Blood Calibration Standard, 5 x 1 ml (lyoph.), (1x) |
| Method of Analysis | HPLC |
|---|---|
| Sample Preparations | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | 0.8 – 750 μg/l |
| Specimen | Whole Blood |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. • Pipette 200 µl sample/calibrator/control into an amber-coloured reaction vial. |
| Run Time | 6 min |
| Injection Volume | 50 µl |
| Flow Rate | 1 ml/min |
| Column Temperature | +20 to +25 °C |
| Gradient | isocratic |
| Wavelengths | EX 367 nm |
| Additional Info | Every isocratic HPLC system with fluorescence detector is suitable. |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate) |
35000-BK Vitamin B1 in Whole Blood – HPLC Basic Kit
For 100 sample preparations
| Order No. | Reagent Kit components (Quantities in kit) |
| 37003 | Extraction Buffer, 10 ml (1x) |
| 37004 | Precipitation Reagent, 30 ml (1x) |
| 35005 | Derivatisation Reagent 1, 5 x 0.3 ml (1x) |
| 35006 | Derivatisation Reagent 2, 20 ml (1x) |
| 35007 | Stabilisation Buffer, 10 ml (1x) |
| 35009 | Neutralisation Buffer, 10 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x) |
| Order No. | Required components (not included in the Basic Kit) |
| 35021 | Mobile Phase, 1000 ml |
| 37008 | Whole Blood Calibration Standard, 5 x 1 ml (lyoph.) |
| 35110 | HPLC Column, equilibrated, with test chromatogram, 1 pc. |
| 0034 | Vitamin B1/B2 Whole Blood Control, Level I, 5 x 2 ml |
| 0035 | Vitamin B1/B2 Whole Blood Control, Level II, 5 x 2 ml |
| Order No. | Lab equipment |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. |
| 15010 | PEEK Prefilter Housing, 1 pc. |
| 15009 | PEEK-encased Prefilter, 5 µm, 5 pcs. |
| 18001 | Precolumn Cartridge Holder 4/10, 1 pc. |
| 18010 | Precolumn Cartridge 4/10, 1 pc. |
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC
Vitamin B1 (TPP, thiamine pyrophosphate)
Clinical relevance
This assay allows the quantitative determination of the physiologically active form of vitamin B1, thiamine pyrophosphate, in human whole blood samples by HPLC.
It is intended to be used for determination of vitamin B1 levels where indicated
• in patients with suspected vitamin B1 deficiency,
• in patients with suspected vitamin B1 excess,
• in patients under vitamin B1 supplementation therapy.
Assay characteristics
This assay requires an isocratic HPLC system with fluorescence detector. The usual post-column derivatisation using extensive equipment is replaced by a simple pre-column derivatisation. Sample preparation is restricted to a single precipitation step after addition of the extraction buffer and subsequent derivatisation in a reaction vial.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Two types of kits available
Basic Kit
• Only sample preparation reagents and vials included
• No excess quantities of mobile phase and calibrators
|
35000-BK Basic Kit |
|
| 37003 | Extraction Buffer, 10 ml (1x) |
| 37004 | Precipitation Reagent, 30 ml (1x) |
| 35005 | Derivatisation Reagent 1, 5 x 0.3 ml (1x) |
| 35006 | Derivatisation Reagent 2, 20 ml (1x) |
| 35007 | Stabilisation Buffer, 10 ml (1x) |
| 35009 | Neutralisation Buffer, 10 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x) |
Reagent Kit
• Mobile phase and calibrator included
|
35000 Reagent Kit |
|
| 37003 | Extraction Buffer, 10 ml (1x) |
| 37004 | Precipitation Reagent, 30 ml (1x) |
| 35005 | Derivatisation Reagent 1, 5 x 0.3 ml (1x) |
| 35006 | Derivatisation Reagent 2, 20 ml (1x) |
| 35007 | Stabilisation Buffer, 10 ml (1x) |
| 35009 | Neutralisation Buffer, 10 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x) |
| 35021 | Mobile Phase, 1000 ml, (1x) |
| 37008 | Whole Blood Calibration Standard, 5 x 1 ml (lyoph.), (1x) |
| Method of Analysis | HPLC |
|---|---|
| Sample Preparations | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | 0.8 – 750 μg/l |
| Specimen | Whole Blood |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. • Pipette 200 µl sample/calibrator/control into an amber-coloured reaction vial. |
| Run Time | 6 min |
| Injection Volume | 50 µl |
| Flow Rate | 1 ml/min |
| Column Temperature | +20 to +25 °C |
| Gradient | isocratic |
| Wavelengths | EX 367 nm |
| Additional Info | Every isocratic HPLC system with fluorescence detector is suitable. |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate) |
35000-BK Vitamin B1 in Whole Blood – HPLC Basic Kit
For 100 sample preparations
| Order No. | Reagent Kit components (Quantities in kit) |
| 37003 | Extraction Buffer, 10 ml (1x) |
| 37004 | Precipitation Reagent, 30 ml (1x) |
| 35005 | Derivatisation Reagent 1, 5 x 0.3 ml (1x) |
| 35006 | Derivatisation Reagent 2, 20 ml (1x) |
| 35007 | Stabilisation Buffer, 10 ml (1x) |
| 35009 | Neutralisation Buffer, 10 ml (1x) |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x) |
| Order No. | Required components (not included in the Basic Kit) |
| 35021 | Mobile Phase, 1000 ml |
| 37008 | Whole Blood Calibration Standard, 5 x 1 ml (lyoph.) |
| 35110 | HPLC Column, equilibrated, with test chromatogram, 1 pc. |
| 0034 | Vitamin B1/B2 Whole Blood Control, Level I, 5 x 2 ml |
| 0035 | Vitamin B1/B2 Whole Blood Control, Level II, 5 x 2 ml |
| Order No. | Lab equipment |
| 33005 | Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. |
| 15010 | PEEK Prefilter Housing, 1 pc. |
| 15009 | PEEK-encased Prefilter, 5 µm, 5 pcs. |
| 18001 | Precolumn Cartridge Holder 4/10, 1 pc. |
| 18010 | Precolumn Cartridge 4/10, 1 pc. |
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC
-
Whole Blood Calibration Standard Vitamins B1/B2Order no.: 37008Vitamin B1/B2 in Whole Blood - HPLC