Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate
7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480), suitable for manual and automated sample prep procedure
Clinical relevance
This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS. One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours.
Product advantages
- 7 analytes in one single run
- Fast and efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
- Fully automated workflow solution available
An automated workflow solution is also available.
Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).
This Sample Prep Set encompasses all required components for an efficient sample preparation.
In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantification | 1.0 - 13.5 µg/l |
| Upper Limit of Quantification | 1600 - 5000 µg/l |
| Intraassay | CV = 1.5 - 8.1 % |
| Interassay | CV = 3.1 - 10.7 % |
| Run Time | 4 min |
| Specimen | 24 h urine or spontaneous urine |
| Pre-analytic Treatment | 24 h urine collected in a suitable container with 10 ml of 10–25 % HCl.
Patient specimens are stable for at least 7 days at +2 to +8 °C in amber coloured vials. |
| Sample Preparation |
|
| Sample Stability | The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C. |
| Injection Volume | ≤ 10 µl |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameter | 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin |
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Internal Standard Mix - 5 x 5 mlOrder no.: 80604/5MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Pierceable adhesive seals for 96 Well PlatesOrder no.: 80059MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Chromatographic Platform Starter SetOrder no.: 80000MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
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Tuning Mix MassChrom® Biogenic Amines/MetabolitesOrder no.: 80615MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
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MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480), suitable for manual and automated sample prep procedure
Clinical relevance
This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS. One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours.
Product advantages
- 7 analytes in one single run
- Fast and efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
- Fully automated workflow solution available
An automated workflow solution is also available.
Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).
This Sample Prep Set encompasses all required components for an efficient sample preparation.
In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantification | 1.0 - 13.5 µg/l |
| Upper Limit of Quantification | 1600 - 5000 µg/l |
| Intraassay | CV = 1.5 - 8.1 % |
| Interassay | CV = 3.1 - 10.7 % |
| Run Time | 4 min |
| Specimen | 24 h urine or spontaneous urine |
| Pre-analytic Treatment | 24 h urine collected in a suitable container with 10 ml of 10–25 % HCl.
Patient specimens are stable for at least 7 days at +2 to +8 °C in amber coloured vials. |
| Sample Preparation |
|
| Sample Stability | The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C. |
| Injection Volume | ≤ 10 µl |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameter | 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin |
-
Internal Standard Mix - 5 x 5 mlOrder no.: 80604/5
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
-
-
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 80059
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Chromatographic Platform Starter SetOrder no.: 80000
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
-
-
Tuning Mix MassChrom® Biogenic Amines/MetabolitesOrder no.: 80615
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS