Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate

Noradrenaline (norepinephrine): 4.00 µg/l – 1600 µg/l
Adrenaline (epinephrine): 2.50 µg/l – 1600 µg/l
Dopamine: 13.5 µg/l – 3000 µg/l
Normetanephrine: 3.50 µg/l – 5000 µg/l
Metanephrine: 2.00 µg/l – 5000 µg/l
3-Methoxytyramine: 3.00 µg/l – 2600 µg/l
Serotonin: 2.00 µg/l – 5000 µg/l

Different systems might show different performance data.

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

• Put 96 SPE Well Plate onto a Collection Plate.
• Pipette 200 µl of the sample/calibrator/MassCheck® control into each well of the 96 SPE Well Plate.
• Add 50 µl Internal Standard Mix and 600 µl Neutralisation Buffer.
• Check for correct colour.
• Centrifuge 5 min at 150 x g, discard effluent.
• Add 0.9 ml Wash Buffer 1 and centrifuge 5 min at 150 x g, discard effluent.
• Add 2 x 0.9 ml Wash Buffer 2 and centrifuge each time 1 min at 150 x g, discard effluent
• Centrifuge 1 min at 2000 x g to dryness, discard effluent.
• Place 96 SPE Well Plate onto a new Collection Plate.
• Add 500 µl Elution Buffer to each well and centrifuge 2 min at 100 x g.
• Add 500 µl Dilution Buffer to each well of the Collection Plate.
• Seal Collection Plate with adhesive seal.
• Shake for 2 min at 900 rpm and transfer to autosampler.
• Inject up to 10 µl.