Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate
7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
Adrenaline (epinephrine)
Noradrenaline (norepinephrine)
Dopamine
Free metanephrine
Free normetanephrine
Free 3-methoxytyramine
Serotonin
MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480), suitable for manual and automated sample prep procedure
Clinical relevance
This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS. One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours.
Product advantages
- 7 analytes in one single run
- Fast and efficient sample prep
Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary - Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
- Fully automated workflow solution available
This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.
A fully automated workflow solution is also available.
Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Limit of quantification | 1.0 - 13.5 µg/l |
| Linearity | 1600 - 5000 µg/l |
| Intraassay | CV = 1.5 - 8.1 % |
| Interassay | CV = 3.1 - 10.7 % |
| Analysis Time | 4 min |
| Specimen | 24 h urine or spontaneous urine |
| Pre-analytic Treatment | 24 h urine collected in a suitable container with 10 ml of 10–25 % HCl.
Patient specimens are stable for at least 7 days at +2 to +8 °C in amber coloured vials. |
| Sample Preparation |
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| Sample Stability | The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C. |
| Injection Volume | ≤ 10 µl |
| Ionisation | ESI positive |
| MS/MS-Mode | MRM |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Parameter | 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin |
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Internal Standard Mix - 5 x 5 mlOrder no.: 80604/5MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Pierceable adhesive seals for 96 Well PlatesOrder no.: 80059MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, SerotoninOrder no.: 80639MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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MassCheck® Catecholamines, free Metanephrines, Serotonin Urine ControlsOrder no.: 0372/0373MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Tuning Mix MassChrom® Biogenic Amines/MetabolitesOrder no.: 80615MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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