Parameter Set Mycophenolic Acid - LC-MS/MS

Clinical relevance

Mycophenolic acid (MPA) is used as an immunosuppressant agent to prevent organ transplant rejection. MPA selectively inhibits the synthesis of purines and specifically reduces the growth of B- and T-lymphocytes. Furthermore, MPA is also used to treat autoimmune diseases such as psoriasis, systemic lupus erythematosus or scleroderma.

Therapeutic monitoring of MPA in the blood is required to adjust individual concentrations within the therapeutic window and to attain a favourable ratio between therapeutic effects and side effects. Currently available drugs contain mycophenolate mofetil or mycophenolate sodium, which are both completely metabolised into the active metabolite MPA. For excretion, MPA is bound to glucuronic acid. As this step is reversible, it is advisable to analyse MPA glucuronide (MPAG) in serum/plasma, too.

Product advantages

With the Parameter Set Mycophenolic Acid in serum/plasma, MPA and its metabolite MPAG can be measured fast and efficiently using LC-MS/MS. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) is minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of deuterated internal standards allows this method to produce reproducible results despite the presence of in-source fragmenting, which is characteristic of this substance. The use of 3PLUS1® or 6PLUS1® multilevel calibrators guarantees high precision and reliable quantification. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets.

For the analysis you require the MassTox® TDM Basic Kit A, the specific MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.