Levetiracetam (Keppra®) in Serum/Plasma

Levetiracetam (Keppra®)

Clinical relevance

Levetiracetam (Keppra®) is an antiepileptic that bears no structural similarity to other drugs. In the cells of the brain, Levetiracetam acts to provide presynaptic inhibition over multiple cascades of postsynaptic chloride ion influx.

It is approved for monotherapy of certain forms of epilepsy in patients of age 16 years and older. It may also be prescribed as add-on therapy for younger patients, e.g. for juvenile myoclonic epilepsy (also called Janz syndrome). The determination of the serum concentration of levetiracetam is important since it undergoes individual fluctuations which vary from patient to patient.

Product advantages

Completion of the antiepileptic analysis
Efficient removal of interferences
Exact quantification with a tailored internal standard

This kit allows for the rapid and reliable determination of levetiracetam levels in serum or plasma. Sample preparation is based on an efficient and fast method of solid phase extraction (SPE). Interference from other antiepileptics or their metabolites is prevented. A tailored internal standard is used to achieve an exact quantification. This kit enables isocratic elution for chromatographic separation on an RP column with subsequent UV detection.

Another HPLC kit (order no. 22000) that can determine further antiepileptics in serum/plasma is also available. Additionally a TDM Parameter Set from the MassTox® TDM Series A is available that uses mass spectrometry for analysis and which can measure 26 antiepileptics.

Technical Data

More Information
Method of Analysis	HPLC
Number of Tests	100
Limit of quantification	0.5 mg/l
Linearity	up to 1000 mg/l
Recovery	90 %
Intraassay	CV ≤ 1.3 %
Interassay	CV ≤ 3.7 %
Analysis Time	7 min
Specimen	Serum/Plasma
Pre-analytic Treatment	Patient samples should be kept cool for transport. They are stable up to 1 week at +2 to +8 °C. For longer storage keep samples below -18 °C.
Sample Preparation	Extraktion: Condition the sample clean up columns with 1 ml Equilibration Buffer 1 and 1 ml Equilibration Buffer 2 (centrifugation 1 min at 850 x g). Apply 100 µl serum/plasma followed by 100 µl Internal Standard to the sample clean up column, mix briefly. Draw through completely by centrifugation (3 min at 1580 x g), discard effluent. Wash: Apply 1 ml of Wash Buffer 1, centrifuge 1 min at 1580 x g, discard effluent. Repeat with 1 ml of Wash Buffer 2. Elution: Change collection vial. Apply 500 µl Elution Buffer to the column and draw through completely by centrifugation (1 min at 850 x g). Inject 10 µl eluate into the HPLC system.
Sample Stability	The prepared samples are stable for 3 days at room temperature, at +2 to +8 °C for 1 week. For longer storage keep samples below -18 °C
Injection Volume	10 µl
Flow rate	1.2 ml/min
Column temperature	ambient (~ 25 °C)
Wavelength	210 nm
Gradient	isocratic
Additional Info	Any isocratic HPLC system with UV detector is suitable.
Please note	The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter	Levetiracetam (Keppra®)

Components

Mobile Phase
Order no.: 24001

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Mobile Phase, 10 x 1000 ml
Order no.: 24002

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Levetiracetam Serum Calibration Standard
Order no.: 24003

Levetiracetam in Serum/Plasma - HPLC

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Internal Standard
Order no.: 24004

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Equilibration Buffer 1
Order no.: 24005

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Equilibration Buffer 2
Order no.: 24006

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Wash Buffer 1
Order no.: 24007

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Wash Buffer 2
Order no.: 24009

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Elution Buffer
Order no.: 24010

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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Sample Clean Up Columns
Order no.: 24008

Levetiracetam (Keppra^®) in Serum/Plasma - HPLC

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