Levetiracetam (Keppra®) in Serum/Plasma - HPLC

Order No.: 24000, for 100 tests
Parameters:
Levetiracetam (Keppra®)

Completes the analysis of antiepileptic drugs
Efficient removal of interferences
High sample throughput

Levetiracetam (Keppra®)

Clinical relevance

Levetiracetam (Keppra®) is an antiepileptic that bears no structural similarity to other drugs. In the cells of the brain, Levetiracetam acts to provide presynaptic inhibition over multiple cascades of postsynaptic chloride ion influx.

It is approved for monotherapy of certain forms of epilepsy in patients of age 16 years and older. It may also be prescribed as add-on therapy for younger patients, e.g. for juvenile myoclonic epilepsy (also called Janz syndrome). The determination of the serum concentration of levetiracetam is important since it undergoes individual fluctuations which vary from patient to patient.

 

Product advantages

  • Completion of the antiepileptic analysis
  • Efficient removal of interferences
  • Exact quantification with a tailored internal standard

This kit allows for the rapid and reliable determination of levetiracetam levels in serum or plasma. Sample preparation is based on an efficient and fast method of solid phase extraction (SPE). Interference from other antiepileptics or their metabolites is prevented. A tailored internal standard is used to achieve an exact quantification. This kit enables isocratic elution for chromatographic separation on an RP column with subsequent UV detection.

Another HPLC kit (order no. 22000) that can determine further antiepileptics in serum/plasma is also available. Additionally a TDM Parameter Set from the MassTox® TDM Series A is available that uses mass spectrometry for analysis and which can measure 26 antiepileptics.

More Information
Number of Tests 100
Limit of quantification 0.5 mg/l
Linearity up to 1000 mg/l
Recovery 90 %
Intraassay

CV ≤ 1.3 %

Interassay

CV ≤ 3.7 %

Analysis Time 7 min
Sample Preparation

Extraktion:

  • Condition the sample clean up columns with 1 ml Equilibration Buffer 1 and 1 ml Equilibration Buffer 2 (centrifugation 1 min at 850 x g).
  • Apply 100 µl serum/plasma followed by 100 µl Internal Standard to the sample clean up column, mix briefly.
  • Draw through completely by centrifugation (3 min at 1580 x g), discard effluent.

Wash:

  • Apply 1 ml of Wash Buffer 1, centrifuge 1 min at 1580 x g, discard effluent. Repeat with 1 ml of Wash Buffer 2.

Elution:

  • Change collection vial. Apply 500 µl Elution Buffer to the column and draw through completely by centrifugation (1 min at  850 x g).
  • Inject 10 µl eluate into the HPLC system.
Sample Stability The prepared samples are stable for 3 days at room temperature, at +2 to +8 °C for 1 week. For longer storage keep samples below -18 °C
Specimen Serum/Plasma
Pre-analytic Treatment

Patient samples should be kept cool for transport. They are stable up to 1 week at +2 to +8 °C. For longer storage keep samples below -18 °C.

 

Injection Volume 10 µl
Flow rate 1.2 ml/min
Column temperature ambient (~ 25 °C)
Wavelength 210 nm
Gradient isocratic
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Levetiracetam (Keppra®)
Complete Specification
Linerity: up to 1000 mg/l
Limit of quantification: 0.5 mg/l
Intra-assay: CV < 4 %
Inter-assay: CV < 5 %
Recovery: 90 %
Run time: approx. 4 min

HPLC parameters

For the HPLC analysis of Levetiracetam (Keppra®) any isocratic HPLC system with UV detector is suitable.

Injection volume: 10 µl
Flow rat: 1.5 ml/min
Wavelength: 210 nm
Column temperature: ambient (~25 °C)
Additional Info

Any isocratic HPLC system with UV detector is suitable.

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