Catecholamines in Plasma - HPLC

Order No.: 5000, for 200 tests
Parameters:
Adrenaline (Epinephrine), Dopamine, Noradrenaline

Easy and safe handling
All components are ready-for-use
High sensitivity
Low analysis costs

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation

Adrenaline (Epinephrine)

Dopamine

Noradrenaline

Clinical relevance

The catecholamines adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are biogenic amines, which play a central role in the body as hormones and neurotransmitters. Adrenaline and noradrenaline are produced in the adrenal medulla and the sympathetic nervous system. Dopamine is a neurotransmitter in the brain and acts as a local messenger substance in the peripheral nervous system.

The determination of catecholamines has clinical significance in the diagnosis of pheochromocytomas and other tumours affecting the nervous system. These diseases result in the production of surplus catecholamines, which are then excreted in the urine. 24-hour urine collection is the preferred method for tumour screening. The plasma concentration is determined to localise the tumour or to perform pharmacological function tests. Furthermore, the concentrations of adrenaline and noradrenaline are important indicators for cardiac insufficiency, cardiac diseases and arteriosclerosis.

 

Product advantages

  • Simple and safe handling
  • Low detection limits
  • Low analysis costs
  • Ready-to-use components

 

Our established kit allows the standard routine analysis of adrenaline, noradrenaline and dopamine in plasma with an isocratic HPLC system and electrochemical detector. A very specific sample preparation using solid phase extraction guarantees simple and safe handling of the samples as well as reproducible results.

For the HPLC analysis of catecholamines in urine, there is another method (# 6000) available.

 

Kit content

Mobile Phase, 1000 ml
Extraction Buffer, 100 ml
Catecholamines Calibration Standard, 10 ml
Internal Standard, 10 ml
Wash Buffer, 300 ml
Elution Buffer, 25 ml
Sample Clean Up Columns, 50 pcs.

More Information
Method of Analysis HPLC
Number of Tests 200
Limit of quantification

Adrenaline, Noradrenaline: 18-43 ng/l
Dopamine: 81 ng/l

Linearity

2250–4000 ng/l

Recovery > 70 % (dopamine > 40 %)
Intraassay

CV < 3.8 %

Interassay

CV < 6 % (dopamine ~13 %)

Analysis Time < 20 min
Specimen Plasma
Pre-analytic Treatment

Sample drawing

  • Blood samples should be drawn from the recumbent patient.
  • 20 µl stabilisation solutuion (900 mg EGTA, 700 mg glutathione in 0,5 M NaOH ad 8 ml, pH 7-7.5 with H2O ad 10 ml) is required per ml blood.
  • Mix, centrifuge.
Sample Preparation
  • Add 500 µl Extraction Buffer to a sample clean up cartridge, shake briefly.
  • Apply 1 to 1.5 ml plasma and 50 µl Internal Standard.
  • Mix for 10 min by inverting.
  • Remove the bottom plug and remove supernatant by vacuum or centrifugation, discard effluent.
  • 3 times: apply 1 ml Wash Buffer, shake briefly, centrifuge (1 min at 2000 rpm, after the last wash step 2 min at 4000 rpm), discard effluent.
  • Mount the plastic tube to the outlet of the cartridge, add 120 µl Elution Buffer, shake briefly, incubate for 5 min. Vortex 30 s.
  • Centrifuge for 1 min at 2000 rpm into the attached plastic tube. Collect eluat and mix well.
  • Inject 20-50 µl into the HPLC system.
Sample Stability At ambient temperature up to 2 hours; below -18 °C up to 9 months
Injection Volume 20-50 µl
Flow rate 0.8-1.3 ml/min
Column temperature ambient (~25 °C)
Additional Info

For the HPLC analysis of catecholamines in plasma any isocratic HPLC system with electrochemical detector is suitable.

Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Adrenaline (Epinephrine), Dopamine, Noradrenaline
Potential 400 - 500 mV
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