Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC

Order No.: 25000, for 100 tests
Parameters:
Amiodarone, Desethylamiodarone

Analysis of active metabolite included
Quick and easy sample preparation
Stable calibration standards and controls

Amiodarone

Desethylamiodarone

Clinical relevance

Amiodarone is a typical representative of the class III antiarrhythmic agents that induce prolongation of the action potentials and refractory periods in the heart. It is approved in Germany for the treatment of various tachycardia arrhythmias, and is often prescribed in combination with other beta blockers. Additionally, amiodarone is often prescribed when other medications show no effect or stop working. Although amiodarone itself rarely causes cardiac arrhythmias, adverse effects may occur in other organs, e.g. reversible sensitivity to light in the eyes and the skin with risk of sunburn, increased liver-related values, as well as very rarely lung damages and hyperthyroidism/hypothyroidism. Regular monitoring of the amiodarone levels is therefore essential. Its bioactive metabolite desethylamiodarone can also be used for measurements.

 

Product advantages

  • Metabolite analysis included
  • Simple and fast sample preparation
  • Stable internal standards and quality controls

This kit allows for the simple and reliable HPLC analysis of amiodarone and desethylamiodarone. Sample preparation is based on a simple and very efficient method of precipitation of all of the interfering components of the sample matrix. The separation is then performed on an isocratic HPLC system with UV detector.

The MassTox® TDM Series A Parameter Set for Antiarrhythmic Drugs is also available for using LC-MS/MS to analyse amiodarone and desethylamiodarone.

More Information
Number of Tests 100
Limit of quantification

0.15 mg/l (Amiodarone)
0.10 mg/l (Desethylamiodarone)

Linearity

up to 20 mg/l

Recovery 99–106 %
Intraassay

CV = 0.6–1.1 %

Interassay

CV = 3.4–5.5 %

Analysis Time approx. 11 min
Sample Preparation
  • Pipette 100 µl serum/plasma
    + 150 µl Internal Standard
    + 100 µl Precipitation Reagent into an amber coloured reaction vial (light protection).
  • Mix 20 s (vortex).
  • Centrifuge 10 min at at least 9000 x g.
  • Inject 25 µl of the supernatant into the HPLC system.
Sample Stability Protected from light, the prepared samples are stable at +20 to +25 °C for up to 1 week, at +2 to +8 °C or below -18 °C for up to 2 weeks.
Specimen Serum/Plasma
Pre-analytic Treatment

Patient samples are stable cooled (+4 °C) in the dark for at least 24 hours. For longer storage keep samples at approx. -20 °C.

 

Injection Volume 25 µl
Flow rate 1 ml/min
Column temperature ambient (~ 25 °C)
Wavelength 242 nm
Gradient isocratic
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter Amiodarone, Desethylamiodarone
Additional Info

Any isocratic HPLC system with UV detector is suitable.

  1. 25011 HPLC mobile phase amiodarone desethylamiodarone serum plasma
    Mobile Phase
    Order no.: 25011
    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
    Details
  2. 25011 HPLC mobile phase amiodarone desethylamiodarone serum plasma
    Mobile Phase, 10 x 1000 ml
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    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
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  3. 25003 HPLC precipitation reagent amiodarone desethylamiodarone serum plasma
    Precipitation Reagent
    Order no.: 25033
    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
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  4. 25044 HPLC internal standard amiodarone desethylamiodarone serum plasma
    Internal Standard
    Order no.: 25044
    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
    Details
  5. 25005 HPLC plasma calibration standard amiodarone desethylamiodarone
    Amiodarone, Desethylamiodarone Plasma Calibration Standard
    Order no.: 25005
    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
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  6. Reaction Vials, 1.5 ml
    Reaction Vials, 1.5 ml
    Order no.: 33005
    Amber colour, light protected
    Details
  1. 25005 HPLC plasma calibration standard amiodarone desethylamiodarone
    Amiodarone, Desethylamiodarone Plasma Calibration Standard
    Order no.: 25005
    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. 25100 HPLC column amiodarone desethylamiodarone serum plasma
    HPLC Column Amiodarone and Desethylamiodarone in Serum/Plasma
    Order no.: 25100
    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
    Details
  3. PEEK-encased Prefilter, 5 µm
    Details
  4. Precolumn Cartridge Holder 4/10
    Details
  5. Precolumn cartridge 4/10
    Precolumn cartridge 4/10
    Order no.: 18025
    Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC
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