Parameter Set Antiepileptic Drugs All-in-One Method for automated sample preparation on Chromsystems MassSTAR
CE-IVD compliant automation on Chromsystems MassSTAR
Determination of all 29 analytes in a single run
With 26 internal standards
Part of the continuously extended MassTox® TDM Series A
Brivaracetam
Carbamazepine
Carbamazepine-10,11-epoxide
10,11-Dihydroxycarbamazepine
10-OH-Carbamazepine
Ethosuximide
Felbamate
Gabapentin
Lacosamide
Lamotrigine
Levetiracetam (Keppra®)
N-Desmethylmesuximide
Oxcarbazepine
Perampanel
Phenobarbital
Phenylethylmalonamide (PEMA)
Phenytoin
Pregabalin
Primidone
Retigabine
Rufinamide
Stiripentol
Sultiame
Theophylline
Tiagabine
Topiramate
Valproic acid
Vigabatrin
Zonisamide
Clinical relevance
Epileptic seizures are the result of synchronous discharges of neuron groups in the brain that can lead to sudden and involuntary stereotypical behavioural or sensory disorders. Numerous types of seizures have been described, each of which requires specialised therapy. The probability of them occurring depends on a number of factors. In addition to genetic predisposition, it is primarily exogenous factors that are relevant, such as accident, thrombosis, tumours or meningitis. Therapy with anticonvulsive medication (antiepileptics) leads to a reduction in seizures and sometimes even a complete elimination of seizures in most treated patients. The precondition for the antiepileptics to have a therapeutic effect is usually patient compliance, which means regular use of the medication. Thus, monitoring of the blood levels is essential, especially during initial dose setting.
MassTox® TDM Series A
The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A
More information about MassTox® TDM Series A
MassSTAR
For this parameter set Chromsystems offers a CE-IVD compliant workflow method using the MassSTAR to automate the sample preparation.
More information about MassSTAR
Detailed performance evaluation data can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in the appendices of the instruction manual. |
| Lower Limit of Quantitation | 0.004 – 9 mg/l |
| Upper Limit of Quantification | up to 0.50 – 275 mg/l |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Automated Sample Preparation * Ready to use automation routine provided with the installation by Chromsystems. |
| Run Time | 4.9 min |
| Injection Volume | 0.2 – 30 µl |
| Gradient | binary |
| Ionisation | ESI positive and negative |
| MS/MS Mode | MRM |
| Additional Info | This method further includes 26 internal standards, which are not shown in the chromatogram for clarity. To avoid ESI switching, the analysis of ethosuximide, phenobarbital and valproic acid can be performed separately in ESI negative mode. |
| Parameters | 10,11-Dihydroxycarbamazepine, 10-OH-Carbamazepine, Brivaracetam, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam (Keppra®), N-Desmethylmesuximide, Oxcarbamazepine, Perampanel, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Retigabine, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide |
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3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDEDOrder no.: 92025/XTMassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS -
MassCheck® Antiepileptic Drugs/EXTENDED Plasma ControlsOrder no.: 0249/XT; 0250/XT; 0251/XTMassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS
-
Internal Standard Mix Antiepileptic Drugs/EXTENDEDOrder no.: 92546/XTComponent of the Parameter Set Antiepileptic Drugs All-in-One Method, available separately
-
MassTox® TDM MasterColumn® AOrder no.: 92110Analytical column for MassTox® TDM Series A - LC-MS/MS
-
Basic Kit A for automated sample preparation on Chromsystems MassSTAROrder no.: 92711CE-IVD compliant automation on Chromsystems MassSTAR
Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phases -
Tuning Mix Antiepileptic Drugs/EXTENDED 1Order no.: 92034/XTTuning Mix 1 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS -
Tuning Mix Antiepileptic Drugs/EXTENDED 2Order no.: 92035/XTTuning Mix 2 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS -
Tuning Mix Antiepileptic Drugs/EXTENDED 3Order no.: 92036/XTTuning Mix 3 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS -
Tuning Mix Antiepileptic Drugs/EXTENDED 4Order no.: 92037/XTTuning Mix 4 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS -
Tuning Mix Antiepileptic Drugs/EXTENDED 5Order no.: 92038/XTTuning Mix 5 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
-
3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDEDOrder no.: 92025/XTMassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS
-
MassCheck® Antiepileptic Drugs/EXTENDED Plasma ControlsOrder no.: 0249/XT; 0250/XT; 0251/XT
MassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS
Brivaracetam
Carbamazepine
Carbamazepine-10,11-epoxide
10,11-Dihydroxycarbamazepine
10-OH-Carbamazepine
Ethosuximide
Felbamate
Gabapentin
Lacosamide
Lamotrigine
Levetiracetam (Keppra®)
N-Desmethylmesuximide
Oxcarbazepine
Perampanel
Phenobarbital
Phenylethylmalonamide (PEMA)
Phenytoin
Pregabalin
Primidone
Retigabine
Rufinamide
Stiripentol
Sultiame
Theophylline
Tiagabine
Topiramate
Valproic acid
Vigabatrin
Zonisamide
Clinical relevance
Epileptic seizures are the result of synchronous discharges of neuron groups in the brain that can lead to sudden and involuntary stereotypical behavioural or sensory disorders. Numerous types of seizures have been described, each of which requires specialised therapy. The probability of them occurring depends on a number of factors. In addition to genetic predisposition, it is primarily exogenous factors that are relevant, such as accident, thrombosis, tumours or meningitis. Therapy with anticonvulsive medication (antiepileptics) leads to a reduction in seizures and sometimes even a complete elimination of seizures in most treated patients. The precondition for the antiepileptics to have a therapeutic effect is usually patient compliance, which means regular use of the medication. Thus, monitoring of the blood levels is essential, especially during initial dose setting.
MassTox® TDM Series A
The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A
More information about MassTox® TDM Series A
MassSTAR
For this parameter set Chromsystems offers a CE-IVD compliant workflow method using the MassSTAR to automate the sample preparation.
More information about MassSTAR
Detailed performance evaluation data can be found in the appendices of the instruction manual.
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in the appendices of the instruction manual. |
| Lower Limit of Quantitation | 0.004 – 9 mg/l |
| Upper Limit of Quantification | up to 0.50 – 275 mg/l |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Automated Sample Preparation * Ready to use automation routine provided with the installation by Chromsystems. |
| Run Time | 4.9 min |
| Injection Volume | 0.2 – 30 µl |
| Gradient | binary |
| Ionisation | ESI positive and negative |
| MS/MS Mode | MRM |
| Additional Info | This method further includes 26 internal standards, which are not shown in the chromatogram for clarity. To avoid ESI switching, the analysis of ethosuximide, phenobarbital and valproic acid can be performed separately in ESI negative mode. |
| Parameters | 10,11-Dihydroxycarbamazepine, 10-OH-Carbamazepine, Brivaracetam, Carbamazepine, Carbamazepine-10,11-epoxide, Ethosuximide, Felbamate, Gabapentin, Lacosamide, Lamotrigine, Levetiracetam (Keppra®), N-Desmethylmesuximide, Oxcarbamazepine, Perampanel, Phenobarbital, Phenylethylmalonamide (PEMA), Phenytoin, Pregabalin, Primidone, Retigabine, Rufinamide, Stiripentol, Sultiame, Theophylline, Tiagabine, Topiramate, Valproic Acid, Vigabatrin, Zonisamide |
-
3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDEDOrder no.: 92025/XTMassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS
-
MassCheck® Antiepileptic Drugs/EXTENDED Plasma ControlsOrder no.: 0249/XT; 0250/XT; 0251/XT
MassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS
-
Internal Standard Mix Antiepileptic Drugs/EXTENDEDOrder no.: 92546/XTComponent of the Parameter Set Antiepileptic Drugs All-in-One Method, available separately
-
MassTox® TDM MasterColumn® AOrder no.: 92110
Analytical column for MassTox® TDM Series A - LC-MS/MS
-
Basic Kit A for automated sample preparation on Chromsystems MassSTAROrder no.: 92711
CE-IVD compliant automation on Chromsystems MassSTAR
Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phases -
Tuning Mix Antiepileptic Drugs/EXTENDED 1Order no.: 92034/XTTuning Mix 1 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
-
Tuning Mix Antiepileptic Drugs/EXTENDED 2Order no.: 92035/XTTuning Mix 2 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
-
Tuning Mix Antiepileptic Drugs/EXTENDED 3Order no.: 92036/XTTuning Mix 3 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
-
Tuning Mix Antiepileptic Drugs/EXTENDED 4Order no.: 92037/XTTuning Mix 4 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
-
Tuning Mix Antiepileptic Drugs/EXTENDED 5Order no.: 92038/XTTuning Mix 5 for the Parameter Sets Antiepileptic Drugs - LC-MS/MS
-
3PLUS1® Multilevel Plasma Calibrator Set Antiepileptic Drugs/EXTENDEDOrder no.: 92025/XTMassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS
-
MassCheck® Antiepileptic Drugs/EXTENDED Plasma ControlsOrder no.: 0249/XT; 0250/XT; 0251/XT
MassTox® TDM Series A Antiepileptic Drugs in Serum/Plasma – LC-MS/MS