Amiodarone and Desethylamiodarone in Serum/Plasma - HPLC

Clinical relevance

Amiodarone is a typical representative of the class III antiarrhythmic agents that induce prolongation of the action potentials and refractory periods in the heart. It is approved in Germany for the treatment of various tachycardia arrhythmias, and is often prescribed in combination with other beta blockers. Additionally, amiodarone is often prescribed when other medications show no effect or stop working. Although amiodarone itself rarely causes cardiac arrhythmias, adverse effects may occur in other organs, e.g. reversible sensitivity to light in the eyes and the skin with risk of sunburn, increased liver-related values, as well as very rarely lung damages and hyperthyroidism/hypothyroidism. Regular monitoring of the amiodarone levels is therefore essential. Its bioactive metabolite desethylamiodarone can also be used for measurements.

Product advantages

• Metabolite analysis included
• Simple and fast sample preparation
• Stable internal standards and quality controls

This assay allows for the simple and reliable HPLC analysis of amiodarone and desethylamiodarone. Sample preparation is based on a simple and very efficient method of precipitation of all of the interfering components of the sample matrix. The separation is then performed on an isocratic HPLC system with UV detector.

The MassTox® TDM Series A Parameter Set for Antiarrhythmic Drugs is also available for using LC-MS/MS to analyse amiodarone and desethylamiodarone.