Vitamin B1 in Whole Blood - HPLC Reagent Kit

Order No.: 35000, for 100 tests
Parameters:
Vitamin B1 (TPP, thiamine pyrophosphate)
Assay validated according to IVDR (=> Declaration of Conformity)

Vitamin B1 (TPP, thiamine pyrophosphate)

Clinical relevance
This assay allows the quantitative determination of the physiologically active form of vitamin B1, thiamine pyrophosphate, in human whole blood samples by HPLC.

It is intended to be used for determination of vitamin B1 levels where indicated
• in patients with suspected vitamin B1 deficiency,
• in patients with suspected vitamin B1 excess,
• in patients under vitamin B1 supplementation therapy.

 

Assay characteristics
This assay requires an isocratic HPLC system with fluorescence detector. The usual post-column derivatisation using extensive equipment is replaced by a simple pre-column derivatisation. Sample preparation is restricted to a single precipitation step after addition of the extraction buffer and subsequent derivatisation in a reaction vial.

Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.

 

Two types of kits available

Basic Kit
• Only sample preparation reagents and vials included
• No excess quantities of mobile phase and calibrators

35000-BK Basic Kit
For 100 sample preparations

37003 Extraction Buffer, 10 ml (1x)
37004 Precipitation Reagent, 30 ml (1x)
35005 Derivatisation Reagent 1, 5 x 0.3 ml (1x)
35006 Derivatisation Reagent 2, 20 ml (1x)
35007 Stabilisation Buffer, 10 ml (1x)
35009 Neutralisation Buffer, 10 ml (1x)
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x)

Reagent Kit
• Mobile phase and calibrator included
 

35000 Reagent Kit
For 100 tests

37003 Extraction Buffer, 10 ml (1x)
37004 Precipitation Reagent, 30 ml (1x)
35005 Derivatisation Reagent 1, 5 x 0.3 ml (1x)
35006 Derivatisation Reagent 2, 20 ml (1x)
35007 Stabilisation Buffer, 10 ml (1x)
35009 Neutralisation Buffer, 10 ml (1x)
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x)
35021 Mobile Phase, 1000 ml, (1x)
37008 Whole Blood Calibration Standard, 5 x 1 ml (lyoph.), (1x)
More Information
Method of Analysis HPLC
Number of Tests 100
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation 0.8 – 750 μg/l
Specimen Whole Blood
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

• Pipette 200 µl sample/calibrator/control into an amber-coloured reaction vial.
• Add 100 µl Extraction Buffer, mix 2 s (vortex).
• Add 300 µl Precipitation Reagent, mix 30 s (vortex).
• Centrifuge 5 min.
• Pipette 200 µl of the derivatisation mix into a new amber-coloured reaction vial.
• Add 100 µl of the supernatant, mix briefly.
• Add 100 µl Neutralisation Buffer. 
• Add 100 µl Stabilisation Buffer, mix briefly.
• Let stand for 20 min.
• Inject 50 µl into the HPLC system.

Run Time 6 min
Injection Volume 50 µl
Flow Rate 1 ml/min
Column Temperature +20 to +25 °C
Gradient isocratic
Wavelengths

EX 367 nm
EM 435 nm

Additional Info Every isocratic HPLC system with fluorescence detector is suitable.
Parameters Vitamin B1 (TPP, thiamine pyrophosphate)

35000 Vitamin B1 in Whole Blood – HPLC Reagent Kit
For 100 tests

Order No. Reagent Kit components (Quantities in kit)
37003 Extraction Buffer, 10 ml (1x)
37004 Precipitation Reagent, 30 ml (1x)
35005 Derivatisation Reagent 1, 5 x 0.3 ml (1x)
35006 Derivatisation Reagent 2, 20 ml (1x)
35007 Stabilisation Buffer, 10 ml (1x)
35009 Neutralisation Buffer, 10 ml (1x)
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x)
35021 Mobile Phase, 1000 ml, (1x)
37008 Whole Blood Calibration Standard, 5 x 1 ml (lyoph.), (1x)

 

Order No. Required components (not included in the Reagent Kit)
35110 HPLC Column, equilibrated, with test chromatogram, 1 pc.
0034 Vitamin B1/B2 Whole Blood Control, Level I, 5 x 2 ml
0035 Vitamin B1/B2 Whole Blood Control, Level II, 5 x 2 ml

 

Order No. Lab equipment
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs.
15010 PEEK Prefilter Housing, 1 pc.
15009 PEEK-encased Prefilter, 5 µm, 5 pcs.
18001 Precolumn Cartridge Holder 4/10, 1 pc.
18010 Precolumn Cartridge 4/10, 1 pc.
The following components are included in the kit:
As a customer please login or register to gain full access.

Vitamin B1 (TPP, thiamine pyrophosphate)

Clinical relevance
This assay allows the quantitative determination of the physiologically active form of vitamin B1, thiamine pyrophosphate, in human whole blood samples by HPLC.

It is intended to be used for determination of vitamin B1 levels where indicated
• in patients with suspected vitamin B1 deficiency,
• in patients with suspected vitamin B1 excess,
• in patients under vitamin B1 supplementation therapy.

 

Assay characteristics
This assay requires an isocratic HPLC system with fluorescence detector. The usual post-column derivatisation using extensive equipment is replaced by a simple pre-column derivatisation. Sample preparation is restricted to a single precipitation step after addition of the extraction buffer and subsequent derivatisation in a reaction vial.

Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.

 

Two types of kits available

Basic Kit
• Only sample preparation reagents and vials included
• No excess quantities of mobile phase and calibrators

35000-BK Basic Kit
For 100 sample preparations

37003 Extraction Buffer, 10 ml (1x)
37004 Precipitation Reagent, 30 ml (1x)
35005 Derivatisation Reagent 1, 5 x 0.3 ml (1x)
35006 Derivatisation Reagent 2, 20 ml (1x)
35007 Stabilisation Buffer, 10 ml (1x)
35009 Neutralisation Buffer, 10 ml (1x)
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x)

Reagent Kit
• Mobile phase and calibrator included
 

35000 Reagent Kit
For 100 tests

37003 Extraction Buffer, 10 ml (1x)
37004 Precipitation Reagent, 30 ml (1x)
35005 Derivatisation Reagent 1, 5 x 0.3 ml (1x)
35006 Derivatisation Reagent 2, 20 ml (1x)
35007 Stabilisation Buffer, 10 ml (1x)
35009 Neutralisation Buffer, 10 ml (1x)
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x)
35021 Mobile Phase, 1000 ml, (1x)
37008 Whole Blood Calibration Standard, 5 x 1 ml (lyoph.), (1x)
More Information
Method of Analysis HPLC
Number of Tests 100
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation 0.8 – 750 μg/l
Specimen Whole Blood
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

• Pipette 200 µl sample/calibrator/control into an amber-coloured reaction vial.
• Add 100 µl Extraction Buffer, mix 2 s (vortex).
• Add 300 µl Precipitation Reagent, mix 30 s (vortex).
• Centrifuge 5 min.
• Pipette 200 µl of the derivatisation mix into a new amber-coloured reaction vial.
• Add 100 µl of the supernatant, mix briefly.
• Add 100 µl Neutralisation Buffer. 
• Add 100 µl Stabilisation Buffer, mix briefly.
• Let stand for 20 min.
• Inject 50 µl into the HPLC system.

Run Time 6 min
Injection Volume 50 µl
Flow Rate 1 ml/min
Column Temperature +20 to +25 °C
Gradient isocratic
Wavelengths

EX 367 nm
EM 435 nm

Additional Info Every isocratic HPLC system with fluorescence detector is suitable.
Parameters Vitamin B1 (TPP, thiamine pyrophosphate)

35000 Vitamin B1 in Whole Blood – HPLC Reagent Kit
For 100 tests

Order No. Reagent Kit components (Quantities in kit)
37003 Extraction Buffer, 10 ml (1x)
37004 Precipitation Reagent, 30 ml (1x)
35005 Derivatisation Reagent 1, 5 x 0.3 ml (1x)
35006 Derivatisation Reagent 2, 20 ml (1x)
35007 Stabilisation Buffer, 10 ml (1x)
35009 Neutralisation Buffer, 10 ml (1x)
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs. (2x)
35021 Mobile Phase, 1000 ml, (1x)
37008 Whole Blood Calibration Standard, 5 x 1 ml (lyoph.), (1x)

 

Order No. Required components (not included in the Reagent Kit)
35110 HPLC Column, equilibrated, with test chromatogram, 1 pc.
0034 Vitamin B1/B2 Whole Blood Control, Level I, 5 x 2 ml
0035 Vitamin B1/B2 Whole Blood Control, Level II, 5 x 2 ml

 

Order No. Lab equipment
33005 Reaction Vials, 1.5 ml, amber colour (light protection), 100 pcs.
15010 PEEK Prefilter Housing, 1 pc.
15009 PEEK-encased Prefilter, 5 µm, 5 pcs.
18001 Precolumn Cartridge Holder 4/10, 1 pc.
18010 Precolumn Cartridge 4/10, 1 pc.
The following components are included in the kit:
As a customer please login or register to gain full access.