Antibiotics in Serum/Plasma - HPLC

Order No.: 61000, for 100 tests
Parameters:
Ampicillin, Cefepime, Ceftazidime, Linezolid, Meropenem, Piperacillin, Sulbactam, Tazobactam

Simple to perform tests
One column and one sample prep for all parameters
Stable calibrators and controls available

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Ampicillin

Cefepime

Ceftazidime

Linezolid

Meropenem

Piperacillin

Sulbactam*

Tazobactam*

 

* For performance evaluation only

The assay Antibiotics in Serum/Plasma is an in vitro diagnostic product for the use in clinical laboratories. It is used for the quantitative determination of ampicillin, cefepime, ceftazidime, linezolid, meropenem and piperacillin in human serum and plasma samples for monitoring patients’ blood levels of these antibiotics. The calibrators and controls also include Sulbactam* and Tazobactam* for obtaining qualitative data (customer validation required).

The HPLC determination with subsequent UV detection is done in two groups, the first run via a mobile phase gradient, the second run isocratically. Two internal standards and stable matrix products ensure precise quantification. The additionally included Priming Solution increases sample stability notably.

* Qualitative information on Sulbactam and Tazobactam can be obtained, but data are not CE-IVD compliant.

 

Further Information: Therapeutic Drug Monitoring in Intensive Care

Read more about the importance of appropriate drug concentrations in antibiotic therapies to and help prevent over- and underdosing. This approach also reduces the risk of new multidrug resistant strains emerging and helps to provide the most effective drug dose to the patient.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 0.5–2.2 mg/l
Upper Limit of Quantification up to 60–400 mg/l
Intraassay CV = 0.5–1.6 %
Interassay CV = 2.5–5.5 %
Recovery 91–104 %
Specimen Serum/Plasma
Sample Preparation
  • Pipette 100 μl sample into a 1.5 ml reaction vial.
  • Add 20 μl Priming Solution, mix briefly (vortex).
  • Add 200 μl Internal Standard, mix 1 min (vortex), centrifuge 5 min.
  • Transfer 100 μl supernatant into autosampler vial with insert.
  • Add 100 μl Dilution Buffer, mix briefly (vortex).
  • Inject 5 μl (group 1) or 10 μl (group 2) into HPLC system.
Run Time 13 min (Group 1), 5 min (Group 2)
Injection Volume 5 μl (Group 1), 10 μl (Group 2)
Flow Rate 1 ml/min
Column Temperature 22 °C
Gradient

binary (Group 1)
isocratic (Group 2)

Wavelengths

0–5.9 min 290 nm, then 210 nm (Group 1)
252 nm (Group 2)

Additional Info A binary HPLC gradient system with coolable injector, coolable column oven and UV-detector is required.
Information Autosampler temperature: 10 °C
Parameters Ampicillin, Cefepime, Ceftazidime, Linezolid, Meropenem, Piperacillin, Sulbactam, Tazobactam
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

Ampicillin

Cefepime

Ceftazidime

Linezolid

Meropenem

Piperacillin

Sulbactam*

Tazobactam*

 

* For performance evaluation only

The assay Antibiotics in Serum/Plasma is an in vitro diagnostic product for the use in clinical laboratories. It is used for the quantitative determination of ampicillin, cefepime, ceftazidime, linezolid, meropenem and piperacillin in human serum and plasma samples for monitoring patients’ blood levels of these antibiotics. The calibrators and controls also include Sulbactam* and Tazobactam* for obtaining qualitative data (customer validation required).

The HPLC determination with subsequent UV detection is done in two groups, the first run via a mobile phase gradient, the second run isocratically. Two internal standards and stable matrix products ensure precise quantification. The additionally included Priming Solution increases sample stability notably.

* Qualitative information on Sulbactam and Tazobactam can be obtained, but data are not CE-IVD compliant.

 

Further Information: Therapeutic Drug Monitoring in Intensive Care

Read more about the importance of appropriate drug concentrations in antibiotic therapies to and help prevent over- and underdosing. This approach also reduces the risk of new multidrug resistant strains emerging and helps to provide the most effective drug dose to the patient.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 0.5–2.2 mg/l
Upper Limit of Quantification up to 60–400 mg/l
Intraassay CV = 0.5–1.6 %
Interassay CV = 2.5–5.5 %
Recovery 91–104 %
Specimen Serum/Plasma
Sample Preparation
  • Pipette 100 μl sample into a 1.5 ml reaction vial.
  • Add 20 μl Priming Solution, mix briefly (vortex).
  • Add 200 μl Internal Standard, mix 1 min (vortex), centrifuge 5 min.
  • Transfer 100 μl supernatant into autosampler vial with insert.
  • Add 100 μl Dilution Buffer, mix briefly (vortex).
  • Inject 5 μl (group 1) or 10 μl (group 2) into HPLC system.
Run Time 13 min (Group 1), 5 min (Group 2)
Injection Volume 5 μl (Group 1), 10 μl (Group 2)
Flow Rate 1 ml/min
Column Temperature 22 °C
Gradient

binary (Group 1)
isocratic (Group 2)

Wavelengths

0–5.9 min 290 nm, then 210 nm (Group 1)
252 nm (Group 2)

Additional Info A binary HPLC gradient system with coolable injector, coolable column oven and UV-detector is required.
Information Autosampler temperature: 10 °C
Parameters Ampicillin, Cefepime, Ceftazidime, Linezolid, Meropenem, Piperacillin, Sulbactam, Tazobactam
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.