Serotonin in Urine - HPLC
Now easier pH adjustment with pH indicator
Easy sample preparation
Short analysis time
Serotonin (5-hydroxytryptamine) is a biogenic amine. In humans, serotonin is involved in a large number of physiological processes, e.g. it acts as a tissue hormone and neurotransmitter in the central nervous system, the enteric nervous system and the cardiovascular system. A large amount of serotonin is found in the chromaffin cells of the gastrointestinal tract where it is part of the gastrointestinal hormone system. When released into the capillary blood, serotonin is almost absorbed by the thrombocytes.
A number of diseases are associated with pathological changes in the serotonin metabolism. For the routine, the diagnosis of carcinoid tumours together with the analysis of 5-hydroxyindoleacetic acid (5-HIAA) is very important.
- Simple and efficient sample preparation
- Fast results due to short analysis times
- Long column life times
This kit allows for reliable HPLC determination of serotonin in urine as part of routine diagnostic laboratory testing. Easy and fast sample preparation leads to exact and reproducible results. The chromatographic determination requires an isocratic HPLC system with electrochemical detection. Calibrator and quality controls are available separately – they are produced from pure human matrices to prevent matrix-associated measurement variabilities.
Alternatively, Chromsystems offers a kit for determining serotonin in serum/plasma and whole blood (# 3030).
Mobile Phase, 1000 ml
Serotonin Calibration Standard, 10 ml
Internal Standard, 10 ml
Neutralisation Buffer, 300 ml
Wash Buffer, 300 ml
Elution Buffer, 500 ml
Sample Clean Up Columns, 100 pcs.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||5 µg/l|
|Linearity||up to 1000 µg/l|
|Recovery||> 94 %|
CV < 2 %
CV < 3 %
|Analysis Time||< 6 min|
|Specimen||24 h urine (containing 10 ml 25 % HCl) or spontaneous urine (refer data to urine creatinine).|
The following food and drugs can falsify the results of the determination: pineapples, eggplants, avocados, bananas, redcurrants, kiwis, melons, mirabelles, gooseberries, tomatoes, walnuts, plums, methocarbamol, mephenesin, guaifenesin, paracetamol, salicylic acid.
|Sample Stability||At +2 to +8 °C at least 5 days. Aliquot and freeze for longer storage.|
|Injection Volume||20-50 µl|
|Flow rate||1 ml/min|
|Column temperature||ambient (~25 °C)|
For the HPLC analysis of serotonin in urine any isocratic HPLC system with electrochemical detector is suitable.
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Potential||approx. +400 to +500 mV|