Sample Prep Set VMA, HVA, 5-HIAA with Collection Plates
Simple and fast sample prep
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
5-Hydroxyindoleacetic Acid (5-HIAA)
Homovanillic Acid (HVA)
Vanillylmandelic Acid (VMA)
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With collection plates for 96 tests (80800/96) or 480 tests (80800/480), suitable for manual and automated sample preparation
Clinical Relevance
This Sample Prep Set allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS. It is used for the diagnostics of neuroblastoma and carcinoid tumours.
Product advantage
- Simple and fast sample prep consisting of 2 pipetting steps and subsequent injection
- Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
- Fully automated workflow solution available
An automated workflow solution is also available.
Alternatively, there is also a method available using autosampler vials (80800).
This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.
In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantification | 0.2 - 2.6 mg/l |
| Upper Limit of Quantification | VMA, 5-HIAA up to 150 mg/l |
| Recovery | 95 - 113 % |
| Intraassay | CV 2.5 - 6.7 % |
| Interassay | CV 3.7 - 7.7 % |
| Run Time | 3 min |
| Specimen | 24h urine or spontaneous urine |
| Pre-analytic Treatment | 24 h urine is collected in a suitable container with 10 ml of 10–25 % HCl (VMA, HVA) or 10 ml glacial acetic acid (5-HIAA). Patient specimens are stable for up to 14 days at +2 to +8 °C in amber coloured vials. |
| Sample Preparation |
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| Sample Stability | The prepared samples are stable for up to 5 days (VMA and HVA) or up to 3 days ( 5-HIAA) at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C. |
| Injection Volume | ≤ 2 µl |
| Column Temperature | +25 °C |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameter | 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA) |
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Pierceable adhesive seals for 96 Well PlatesOrder no.: 80059
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Chromatographic Platform Starter SetOrder no.: 80000MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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System Check Solution SetOrder no.: 80810MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Tuning Mix VMA, HVA, 5-HIAAOrder no.: 80815MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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Analytical Column VMA, HVA, 5-HIAA Urine - LC-MS/MSOrder no.: 80200MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
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6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
5-Hydroxyindoleacetic Acid (5-HIAA)
Homovanillic Acid (HVA)
Vanillylmandelic Acid (VMA)
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With collection plates for 96 tests (80800/96) or 480 tests (80800/480), suitable for manual and automated sample preparation
Clinical Relevance
This Sample Prep Set allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS. It is used for the diagnostics of neuroblastoma and carcinoid tumours.
Product advantage
- Simple and fast sample prep consisting of 2 pipetting steps and subsequent injection
- Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
- Fully automated workflow solution available
An automated workflow solution is also available.
Alternatively, there is also a method available using autosampler vials (80800).
This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.
In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantification | 0.2 - 2.6 mg/l |
| Upper Limit of Quantification | VMA, 5-HIAA up to 150 mg/l |
| Recovery | 95 - 113 % |
| Intraassay | CV 2.5 - 6.7 % |
| Interassay | CV 3.7 - 7.7 % |
| Run Time | 3 min |
| Specimen | 24h urine or spontaneous urine |
| Pre-analytic Treatment | 24 h urine is collected in a suitable container with 10 ml of 10–25 % HCl (VMA, HVA) or 10 ml glacial acetic acid (5-HIAA). Patient specimens are stable for up to 14 days at +2 to +8 °C in amber coloured vials. |
| Sample Preparation |
|
| Sample Stability | The prepared samples are stable for up to 5 days (VMA and HVA) or up to 3 days ( 5-HIAA) at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C. |
| Injection Volume | ≤ 2 µl |
| Column Temperature | +25 °C |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameter | 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA) |
-
-
-
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 80059
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Chromatographic Platform Starter SetOrder no.: 80000
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
System Check Solution SetOrder no.: 80810
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Tuning Mix VMA, HVA, 5-HIAAOrder no.: 80815
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Analytical Column VMA, HVA, 5-HIAA Urine - LC-MS/MSOrder no.: 80200
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS