Sample Prep Set VMA, HVA, 5-HIAA with Collection Plates

Order No.: 80800/96, for 96 tests
Parameters:
5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA)

Simple and fast sample prep
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

5-Hydroxyindoleacetic Acid (5-HIAA)

Homovanillic Acid (HVA)

Vanillylmandelic Acid (VMA)

MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With collection plates for 96 tests (80800/96) or 480 tests (80800/480)

 

Clinical Relevance

This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS.

It is used as a test for patients in whom the urine levels of these metabolites are of clinical importance.

 

Product advantage

  • Simple and fast sample prep consisting of pipetting and shaking
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available

Alternatively, there is also a method available using autosampler vials (80800).

 

This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

5-Hydroxyindoleacetic acid (5-HIAA): 2.30 mg/l – 150 mg/l
Homovanillic acid (HVA): 0.30 mg/l – 250 mg/l
Vanillylmandelic acid (VMA): 2.90 mg/l – 150 mg/l

Different systems might show different performance data.

Specimen 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual.
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 50 μl sample/calibrator/MassCheck® control into a well of the collection plate.
  • Add 25 μl Internal Standard Mix.
  • Add 425 μl Stabilisation Buffer.
  • Seal collection plate with adhesive seal.
  • Agitate collection plate for 2 min at 1200 rpm (orbit 2 mm) and transfer to autosampler.
  • Inject eluates into the LC-MS/MS system.
Run Time 3 min
Injection Volume ≤ 2 µl or ≤ 10 µl (mass spectrometer dependent)
Column Temperature +25 °C
Gradient binary
Ionisation ESI positive
MS/MS Mode MRM
Parameters 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

5-Hydroxyindoleacetic Acid (5-HIAA)

Homovanillic Acid (HVA)

Vanillylmandelic Acid (VMA)

MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With collection plates for 96 tests (80800/96) or 480 tests (80800/480)

 

Clinical Relevance

This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS.

It is used as a test for patients in whom the urine levels of these metabolites are of clinical importance.

 

Product advantage

  • Simple and fast sample prep consisting of pipetting and shaking
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available

Alternatively, there is also a method available using autosampler vials (80800).

 

This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual.
Lower and Upper Limit of Quantitation

5-Hydroxyindoleacetic acid (5-HIAA): 2.30 mg/l – 150 mg/l
Homovanillic acid (HVA): 0.30 mg/l – 250 mg/l
Vanillylmandelic acid (VMA): 2.90 mg/l – 150 mg/l

Different systems might show different performance data.

Specimen 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual.
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

  • Pipette 50 μl sample/calibrator/MassCheck® control into a well of the collection plate.
  • Add 25 μl Internal Standard Mix.
  • Add 425 μl Stabilisation Buffer.
  • Seal collection plate with adhesive seal.
  • Agitate collection plate for 2 min at 1200 rpm (orbit 2 mm) and transfer to autosampler.
  • Inject eluates into the LC-MS/MS system.
Run Time 3 min
Injection Volume ≤ 2 µl or ≤ 10 µl (mass spectrometer dependent)
Column Temperature +25 °C
Gradient binary
Ionisation ESI positive
MS/MS Mode MRM
Parameters 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.