Sample Prep Set VMA, HVA, 5-HIAA with Collection Plates

Order No.: 80800/480, for 480 tests
Parameters:
5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA)

Simple and fast sample prep
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

5-Hydroxyindoleacetic Acid (5-HIAA)

Homovanillic Acid (HVA)

Vanillylmandelic Acid (VMA)

MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With collection plates for 96 tests (80800/96) or 480 tests (80800/480), suitable for manual and automated sample preparation

 

Clinical Relevance

This Sample Prep Set allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS. It is used for the diagnostics of neuroblastoma and carcinoid tumours.

 

Product advantage

  • Simple and fast sample prep consisting of 2 pipetting steps and subsequent injection
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
  • Fully automated workflow solution available

An automated workflow solution is also available.

Alternatively, there is also a method available using autosampler vials (80800).

 

This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 480
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 0.2 - 2.6 mg/l
Upper Limit of Quantification

VMA, 5-HIAA up to 150 mg/l
HVA up to 250 mg/l

Recovery 95 - 113 %
Intraassay CV 2.5 - 6.7 %
Interassay CV 3.7 - 7.7 %
Specimen 24h urine or spontaneous urine
Sample Preparation
  • Pipette 50 μl sample/calibrator/MassCheck® control into a well of the collection plate.
  • Add 25 μl Internal Standard Mix.
  • Add 425 μl Stabilisation Buffer.
  • Seal collection plate with adhesive seal.
  • Agitate collection plate for 2 min at 1200 rpm (orbit 2 mm) and transfer to autosampler.
  • Inject eluates into the LC-MS/MS system.
Run Time 3 min
Injection Volume ≤ 2 µl
Column Temperature +25 °C
Ionisation ESI positive
MS/MS Mode MRM
Parameters 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

5-Hydroxyindoleacetic Acid (5-HIAA)

Homovanillic Acid (HVA)

Vanillylmandelic Acid (VMA)

MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With collection plates for 96 tests (80800/96) or 480 tests (80800/480), suitable for manual and automated sample preparation

 

Clinical Relevance

This Sample Prep Set allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS. It is used for the diagnostics of neuroblastoma and carcinoid tumours.

 

Product advantage

  • Simple and fast sample prep consisting of 2 pipetting steps and subsequent injection
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
  • Fully automated workflow solution available

An automated workflow solution is also available.

Alternatively, there is also a method available using autosampler vials (80800).

 

This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 480
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 0.2 - 2.6 mg/l
Upper Limit of Quantification

VMA, 5-HIAA up to 150 mg/l
HVA up to 250 mg/l

Recovery 95 - 113 %
Intraassay CV 2.5 - 6.7 %
Interassay CV 3.7 - 7.7 %
Specimen 24h urine or spontaneous urine
Sample Preparation
  • Pipette 50 μl sample/calibrator/MassCheck® control into a well of the collection plate.
  • Add 25 μl Internal Standard Mix.
  • Add 425 μl Stabilisation Buffer.
  • Seal collection plate with adhesive seal.
  • Agitate collection plate for 2 min at 1200 rpm (orbit 2 mm) and transfer to autosampler.
  • Inject eluates into the LC-MS/MS system.
Run Time 3 min
Injection Volume ≤ 2 µl
Column Temperature +25 °C
Ionisation ESI positive
MS/MS Mode MRM
Parameters 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
As a customer please login or register to gain full access.