Sample Prep Set Total Metanephrines (free + conjugated) with 96 SPE Well Plate

Order No.: 80700/96, for 96 tests
Parameters:
3-Methoxytyramine, Metanephrine, Normetanephrine

Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

3-Methoxytyramine

Metanephrine

Normetanephrine

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plate for 96 analyses (80600/96), suitable for manual and automated sample prep procedure

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of total metanephrine, normetanephrine and 3-methoxytyramine in patient urine samples by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with a suspicion of catecholamine-secreting tumours.

 

Product advantages

  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
  • Fully automated workflow solution available

An automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (80700/C).

 

This Sample Prep Set encompasses all required components for an efficient sample preparation.

In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Upper Limit of Quantification

Metanephrine, Normetanephrine up to 20 mg/l
3-Methoxytyramine up to 10 mg/l

Recovery 92–107 %
Intraassay CV = 2.5–5.6 %
Interassay CV = 3.2–4.9 %
Specimen 24h urine or spontaneous urine
Sample Preparation
  • Pipette 200 μl sample/calibrator/MassCheck® control into each well of the Hydrolysis Plate.
  • Add 50 μl Internal Standard Mix + 25 μl Hydrolysis Buffer.
  • Seal Hydrolysis Plate with pierceable adhesive seal and cover.
  • Hydrolyse in the oven for 45 min at 120 °C.
  • Cool for at least 10 min at +2 to +8 °C.
  • Place a 96 SPE Well Plate onto a Collection Plate.
  • Pipette 50 μl hydrolysate into each well of the 96 SPE Well Plate.
  • Add 600 μl Neutralisation Buffer.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Place 96 SPE Well Plate onto a new Collection Plate.
  • Pipette 500 μl Elution Buffer into each well and centrifuge 2 min at 100 x g.
  • Pipette 500 μl Dilution Buffer into each well of the Collection Plate.
  • Seal with adhesive seal.
  • Shake for 2 min at 900 rpm, transfer to autosampler.
  • Inject up to 20 μl into the LC-MS/MS system.
Run Time 4 min
Injection Volume ≤ 20 µl
Ionisation ESI positive
MS/MS Mode MRM
Parameters 3-Methoxytyramine, Metanephrine, Normetanephrine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
General lab equipment (non-CE-IVD products), available from Chromsystems:
As a customer please login or register to gain full access.

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

3-Methoxytyramine

Metanephrine

Normetanephrine

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plate for 96 analyses (80600/96), suitable for manual and automated sample prep procedure

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of total metanephrine, normetanephrine and 3-methoxytyramine in patient urine samples by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with a suspicion of catecholamine-secreting tumours.

 

Product advantages

  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
  • Fully automated workflow solution available

An automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (80700/C).

 

This Sample Prep Set encompasses all required components for an efficient sample preparation.

In addition to the Sample Prep Set the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.

More Information
Method of Analysis LC-MS/MS
Number of Tests 96
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Upper Limit of Quantification

Metanephrine, Normetanephrine up to 20 mg/l
3-Methoxytyramine up to 10 mg/l

Recovery 92–107 %
Intraassay CV = 2.5–5.6 %
Interassay CV = 3.2–4.9 %
Specimen 24h urine or spontaneous urine
Sample Preparation
  • Pipette 200 μl sample/calibrator/MassCheck® control into each well of the Hydrolysis Plate.
  • Add 50 μl Internal Standard Mix + 25 μl Hydrolysis Buffer.
  • Seal Hydrolysis Plate with pierceable adhesive seal and cover.
  • Hydrolyse in the oven for 45 min at 120 °C.
  • Cool for at least 10 min at +2 to +8 °C.
  • Place a 96 SPE Well Plate onto a Collection Plate.
  • Pipette 50 μl hydrolysate into each well of the 96 SPE Well Plate.
  • Add 600 μl Neutralisation Buffer.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1, centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2, centrifuge each time 1 min at 150 x g, discard effluent.
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Place 96 SPE Well Plate onto a new Collection Plate.
  • Pipette 500 μl Elution Buffer into each well and centrifuge 2 min at 100 x g.
  • Pipette 500 μl Dilution Buffer into each well of the Collection Plate.
  • Seal with adhesive seal.
  • Shake for 2 min at 900 rpm, transfer to autosampler.
  • Inject up to 20 μl into the LC-MS/MS system.
Run Time 4 min
Injection Volume ≤ 20 µl
Ionisation ESI positive
MS/MS Mode MRM
Parameters 3-Methoxytyramine, Metanephrine, Normetanephrine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
General lab equipment (non-CE-IVD products), available from Chromsystems:
As a customer please login or register to gain full access.