Parameter Set - Tricyclic Antidepressants TCA 2 - LC-MS/MS

Order No.: 92920
Parameters:
Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine

Encompasses 7 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

Clomipramine

Norclomipramine

Maprotiline

Normaprotiline

Protriptyline

Trimipramine

Nortrimipramine

Clinical relevance

Tricyclic antidepressants (TCAs) are used to treat psychological conditions such as anxiety, pain syndromes, and phobias, and produce a positive mood enhancing effect. Their pharmacological effect is based on increasing the concentration of dopamine, noradrenaline and serotonin in the synaptic cleft by inhibiting the degradation or reuptake of these neurotransmitters. TCAs are highly lipophilic and are absorbed rapidly from the gastrointestinal tract after oral administration. Even before they reach their site of action in the brain, a fraction is already metabolised in the liver (first-pass effect). Regular determination of the plasma levels can minimise the risk of side effects resulting from overdose. Furthermore, patient compliance can be checked by this procedure to help ensure optimal therapeutic success.

 

Product advantages

With the Parameter Set Tricyclic Antidepressants TCA 2 in serum/plasma, 7 different drugs can be measured quickly and efficiently using LC-MS/MS. Due to the careful optimisation of all kit components as well as the chromatographic separation, matrix effects (“ion suppression”) are minimised and the robustness of the method is enhanced. Sample preparation is based on a protein precipitation step. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and the reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets.

For the analysis you require the MassTox® TDM Basic Kit A, the specific MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.

More Information
Limit of quantification 1.5 – 10 µg/l
Linearity at least twice that of the therapeutic range
Intraassay CV = 1.7 – 4.3 %
Interassay CV = 3.8 – 6.3 %
Analysis Time 3.5 min
Sample Preparation
  • Reconstitute the Internal Standard Mix
  • Add 800 μl Internal Standard mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 μl of mixture A and mix 30 s minimum (vortex) and centrifuge 5 min.
  • Dilute the supernatant with Dilution Buffer prior to injection depending on the instrument sensitivity
Sample Stability Samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.
Specimen Serum/Plasma
Injection Volume 0.2 – 50 µl
Gradient Isocratic (35 % Mobile Phase 1; 65 % Mobile Phase 2)
Ionisation ESI positive
MS/MS-Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine
  1. 92446 92044 LCMS TDM Series A IS TCA
    Internal Standard Set Tricyclic Antidepressants TCA
    Order no.: 92446
    Component of the Parameter Sets Tricyclic Antidepressants 1 and 2, available separately
    Details
  2. 92033 LCMS TDM Series A 3PLUS1 calibrator TCA2
    3PLUS1® Multilevel Plasma Calibrator Set Tricyclic Antidepressants TCA 2
    Order no.: 92033
    Component of the Parameter Set Tricyclic Antidepressants TCA 2, available separately
    Details
  3. 0246 0247 0248 LCMS TDM Series A controls TCA2
    MassCheck® Tricyclic Antidepressants TCA 2 Plasma Controls
    Order no.: 0246/0247/0248

    MassTox® TDM Series A

    Details
  1. 92033 LCMS TDM Series A 3PLUS1 calibrator TCA2
    3PLUS1® Multilevel Plasma Calibrator Set Tricyclic Antidepressants TCA 2
    Order no.: 92033
    Component of the Parameter Set Tricyclic Antidepressants TCA 2, available separately
    Details
  1. 0246 0247 0248 LCMS TDM Series A controls TCA2
    MassCheck® Tricyclic Antidepressants TCA 2 Plasma Controls
    Order no.: 0246/0247/0248

    MassTox® TDM Series A

    Details
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® TDM Series A

    Details
  2. 92023 LCMS TDM Series A tuning mix
    Tuning Mix Tricyclic Antidepressants TCA 2
    Order no.: 92023
    Tuning Mix for the Parameter Set Tricyclic Antidepressants TCA 2 - LC-MS/MS
    Details
  3. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  4. PEEK-encased Prefilter, 2 µm
    Details
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