Parameter Set Tricyclic Antidepressants TCA 2 - LC-MS/MS
Encompasses 7 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
Clomipramine
Norclomipramine
Maprotiline
Normaprotiline
Protriptyline
Trimipramine
Nortrimipramine
Clinical relevance
Tricyclic antidepressants (TCAs) are used to treat psychological conditions such as anxiety, pain syndromes, and phobias, and produce a positive mood enhancing effect. Their pharmacological effect is based on increasing the concentration of dopamine, noradrenaline and serotonin in the synaptic cleft by inhibiting the degradation or reuptake of these neurotransmitters. TCAs are highly lipophilic and are absorbed rapidly from the gastrointestinal tract after oral administration. Even before they reach their site of action in the brain, a fraction is already metabolised in the liver (first-pass effect). Regular determination of the plasma levels can minimise the risk of side effects resulting from overdose. Furthermore, patient compliance can be checked by this procedure to help ensure optimal therapeutic success.
MassTox® TDM Series A
The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A
More information about MassTox® TDM Series A
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | 1.5 – 10 µg/l |
| Upper Limit of Quantification | at least twice that of the therapeutic range |
| Intraassay | CV = 1.7 – 4.3 % |
| Interassay | CV = 3.8 – 6.3 % |
| Specimen | Serum/Plasma |
| Sample Preparation |
|
| Run Time | 3.5 min |
| Injection Volume | 0.2 – 50 µl |
| Gradient | Isocratic (35 % Mobile Phase 1; 65 % Mobile Phase 2) |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Additional Info | We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width. |
| Parameters | Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine |
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3PLUS1® Multilevel Plasma Calibrator Set Tricyclic Antidepressants TCA 2Order no.: 92033MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS -
MassCheck® Tricyclic Antidepressants TCA 2 Plasma ControlsOrder no.: 0246/0247/0248MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS -
Internal Standard Set Tricyclic Antidepressants TCAOrder no.: 92446Component of the Parameter Sets Tricyclic Antidepressants 1 and 2, available separately
-
MassTox® TDM MasterColumn® AOrder no.: 92110Analytical column for MassTox® TDM Series A - LC-MS/MS
-
Tuning Mix Tricyclic Antidepressants TCA 2Order no.: 92023Tuning Mix for the Parameter Set Tricyclic Antidepressants TCA 2 - LC-MS/MS -
Basic Kit A for 200 Tests - LC-MS/MSOrder no.: 92111/200Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesValidated according to IVDR
-
Basic Kit A for 1000 Tests - LC-MS/MSOrder no.: 92111/1000Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesValidated according to IVDR
-
3PLUS1® Multilevel Plasma Calibrator Set Tricyclic Antidepressants TCA 2Order no.: 92033MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS
-
MassCheck® Tricyclic Antidepressants TCA 2 Plasma ControlsOrder no.: 0246/0247/0248MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS
Clomipramine
Norclomipramine
Maprotiline
Normaprotiline
Protriptyline
Trimipramine
Nortrimipramine
Clinical relevance
Tricyclic antidepressants (TCAs) are used to treat psychological conditions such as anxiety, pain syndromes, and phobias, and produce a positive mood enhancing effect. Their pharmacological effect is based on increasing the concentration of dopamine, noradrenaline and serotonin in the synaptic cleft by inhibiting the degradation or reuptake of these neurotransmitters. TCAs are highly lipophilic and are absorbed rapidly from the gastrointestinal tract after oral administration. Even before they reach their site of action in the brain, a fraction is already metabolised in the liver (first-pass effect). Regular determination of the plasma levels can minimise the risk of side effects resulting from overdose. Furthermore, patient compliance can be checked by this procedure to help ensure optimal therapeutic success.
MassTox® TDM Series A
The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A
More information about MassTox® TDM Series A
| Method of Analysis | LC-MS/MS |
|---|---|
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | 1.5 – 10 µg/l |
| Upper Limit of Quantification | at least twice that of the therapeutic range |
| Intraassay | CV = 1.7 – 4.3 % |
| Interassay | CV = 3.8 – 6.3 % |
| Specimen | Serum/Plasma |
| Sample Preparation |
|
| Run Time | 3.5 min |
| Injection Volume | 0.2 – 50 µl |
| Gradient | Isocratic (35 % Mobile Phase 1; 65 % Mobile Phase 2) |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Additional Info | We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width. |
| Parameters | Clomipramine, Maprotiline, Norclomipramine, Normaprotiline, Nortrimipramine, Protriptyline, Trimipramine |
-
3PLUS1® Multilevel Plasma Calibrator Set Tricyclic Antidepressants TCA 2Order no.: 92033MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS
-
MassCheck® Tricyclic Antidepressants TCA 2 Plasma ControlsOrder no.: 0246/0247/0248MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS
-
Internal Standard Set Tricyclic Antidepressants TCAOrder no.: 92446Component of the Parameter Sets Tricyclic Antidepressants 1 and 2, available separately
-
MassTox® TDM MasterColumn® AOrder no.: 92110
Analytical column for MassTox® TDM Series A - LC-MS/MS
-
Tuning Mix Tricyclic Antidepressants TCA 2Order no.: 92023Tuning Mix for the Parameter Set Tricyclic Antidepressants TCA 2 - LC-MS/MS
-
Basic Kit A for 200 Tests - LC-MS/MSOrder no.: 92111/200
Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesValidated according to IVDR
-
Basic Kit A for 1000 Tests - LC-MS/MSOrder no.: 92111/1000
Part of the MassTox® TDM Series A
Modular system for therapeutic drug monitoring
Provides all components required for sample prep and all mobile phasesValidated according to IVDR
-
3PLUS1® Multilevel Plasma Calibrator Set Tricyclic Antidepressants TCA 2Order no.: 92033MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS
-
MassCheck® Tricyclic Antidepressants TCA 2 Plasma ControlsOrder no.: 0246/0247/0248MassTox® TDM Series A Tricyclic Antidepressants TCA 2 in Serum/Plasma – LC-MS/MS