Metanephrines in Urine/Combined analysis - HPLC
Short analysis time < 10 min
Only 1 HPLC column and 1 Mobile Phase for 2020/COMBI and 6000/COMBI
In addition to catecholamines, their metabolic products such as metanephrine and normetanephrine are also important indicators in the diagnosis of various tumours. They have to be determined in addition to the parameters VMA and HVA in order to prevent the risk of false-positive results.
- Short analysis time of less than 10 minutes
- No changes to setup are needed due to identical column and mobile phase
Chromsystems offers an exceptionally sensitive and reliable combined method for detecting pheochromocytomas with an analysis time of less than 10 minutes in the analysis of metanephrine with catecholamines in urine (6000/COMBI). The highly selective separation column and the optimised mobile phase are used for the analysis of both catecholamines and metanephrines, so no change of the chromatographic system is required. The established specific sample preparation using solid phase extraction guarantees simple and safe handling and reproducible results.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||4 µg/l|
|Intraassay||CV = 0.6-2.6 %|
|Interassay||CV = 1.6-2.7 %|
|Analysis Time||< 10 min|
|Specimen||24 h urine (containing 10 ml 25 % HCl)|
|Sample Stability||At +2 to +8 °C at least 5 days. Aliquot and freeze for longer storage.|
|Injection Volume||10 µl|
|Flow rate||1.3 ml/min|
|Column temperature||30 °C|
For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used.
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Parameter||3-Methoxytyramine, Metanephrine, Normetanephrine|