MassChrom® Amino Acids and Acylcarnitines from Dried Blood - LC-MS/MS
Sample prep with derivatisation of the analytes
With 96 well filter plates
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation
||Acylcarnitines and free Carnitine|
Newborn screening aims to detect congenital metabolic disorders early on before they can cause harm to the organism. Included in these diseases are defects of amino acid metabolism such as phenylketonuria (PKU) and maple syrup urine disease (MSUD), defects of fatty acid metabolism and organic acidurias (isovaleric acidemia). If these diseases are not diagnosed, the metabolic disorders can already lead to severe irreversible harm to the newborns within their first few days of life.
Mass spectrometry is the method of choice for diagnostic testing: it enables to check for a broad range of metabolic defects in a single run. An HPLC column is not required due to the high selectivity of LC-MS/MS. This makes it possible to achieve a very precise quantification in a very short analysis time of less than 2 minutes.
- Screening for many metabolic disorders in a single run such as PKU (Phenylketonuria) and MSUD (Maple Syrup Urine Disease)
- Each analyte safeguarded by its own internal standard
This Chromsystems assay allows the fast and reliable determination of amino acids, acylcarnitines and succinylacetone from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the blood spot with subsequent derivatisation. The use of stable isotope labelled internal standards for calibration and measurement ensures reliable and precise quantification of the analytes.
Here's an overview of all our newborn screening assays.
|Method of Analysis||LC-MS/MS|
|Number of Tests||960|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Limit of Quantification||
Amino acids: 0.5-5 µmol/l
Amino acids: 250-2500 µmol/l
|Recovery||Amino acids: 51-94 %, (Acyl-)carnitines: 62-89 %|
Amino acids: CV = 4-10 %
Amino acids: CV = 6-9 %
|Analysis Time||< 2 min|
Specimen collection: whole blood from the heel of the newborn taken onto a filter card. Collection between 24th and 72nd hour of life depending on individual national guideline.
Stability: storage at low humidity (< 30 %) and ambient temperature is sufficient if analysis is performed within 24 to 48 hours. For longer storage deep-freeze samples with humidity excluded below -18 °C.
|Injection Volume||10 µl|
|Flow Rate||20–600 µl/min|
|MS/MS-Mode||Amino acids: MRM / Neutral Loss Scan of 102 (m/z); Acylcarnitines: Parent Ion Scan of 85 (m/z)|
No HPLC column is required.
Mass transitions of analytes and internal standards
|Parameter||Acylcarnitines & free Carnitine, Amino Acids|
|Autosampler rinsing solution||Rinsing Solution (55007)|