MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma for automated sample preparation - LC-MS/MS

Order No.: 62000/1000/F, for 1000 tests
Parameters:
25-OH-Vitamin D2, 25-OH-Vitamin D3

Suitable for different liquid handling devices
Sample tracking, LIMS connectivity and full walkaway operation for improved workflows

25-OH-Vitamin D2

25-OH-Vitamin D3

Clinical relevance

Vitamin D (cholecalciferol) is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.

25-OH-vitamin D3 is the primary metabolite in the human body and the direct precursor of the physiologically active form 1.25-(OH)2-vitamin D3. First-line treatment for vitamin D deficiency is nutritional supplements with vitamin D3 or vitamin D2. Vitamin D2 is easier to synthesise and is widely used as a supplement outside of the EU, and especially in the United States. Therefore, its determination in serum/plasma is also relevant.

 

Product advantages

  • Automated sample preparation in 96 Well Filter Plates
  • 3PLUS1® or 6PLUS1® Multilevel Calibrator Set and MassCheck® Controls
  • Isotopically labelled internal standard

 

This Chromsystems kit allows the determination of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma with LC-MS/MS. 96 samples can be reliably processed in 30 minutes using automated sample preparation. The comparison of manual and automated method shows a strong correlation. The 3PLUS1® and 6PLUS1® multilevel calibrators are optionally available and guarantee a high level of precision of the results.

A variant for manual sample preparation in 96 well filter plates (order no. 62000) is also available.

 

Kit content

Mobile Phase A, 5 x 1000 ml
Mobile Phase B, 5 x 1000 ml
Precipitation Reagent, 5 x 5 ml
Internal Standard, 5 x 40 ml
Rinsing Solution, 2 x 1000 ml
96 Well Filter Plates, 4 x 3 pcs.
Collection Plates, 4 x 3 pcs.
Pierceable Adhesive Seals for 96 Well Plates, 4 x 3 pcs.
MassChrom® Analytical column, 1 pc.
MassChrom® Trap column, 2 pcs.
3PLUS1® Multilevel Serum Calibrator Set (lyoph.), 4 x 1 ml
MassCheck® 25-OH-vitamin D3/D2 Serum Control, Level I (lyoph.), 5 x 1 ml
MassCheck® 25-OH-vitamin D3/D2 Serum Control, Level II (lyoph.), 5 x 1 ml

 

More Information
Number of Tests 1000
Limit of quantification

25-OH-Vitamin D2: 2.2 µg/l
25-OH-Vitamin D3: 3.0 µg/l

Linearity

up to 250 μg/l

Intraassay

CV < 5 %

Interassay

CV < 6 %

Analysis Time 5 min
Sample Preparation Samples can be prepared with full or partial automation on a liquid handling device. Manual sample preparation using 96 well filter plates is also possible. A multichannel pipette (8-channel or 12-channel) or a Multipette may be used.
Sample Stability Samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.
Specimen Serum/Plasma
Pre-analytic Treatment

Patient samples are stable up to 3 days at ambient temperature and up to 1 week at +2 to +8 °C. For longer storage (maximum 1 month) deep-freeze the samples below -18 °C.

Ionisation APCI positive
MS/MS-Mode MRM
Information

The trap column and the analytical column are linked to the mass spectrometer through a 2-position 6-port valve.

Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 25-OH-Vitamin D2, 25-OH-Vitamin D3
Additional Info

Pump A delivers 100 % Mobile Phase A for loading the trap column.
Pump B delivers 100 % Mobile Phase A for separating analytes on the analytical column.

Flow

Pump A: 0.02–1.00 ml/min
Pump B: 0.60–1.00 ml/min

  1. 62001 LCMS Vitamin D Mobile Phase A
    Mobile Phase A
    Order no.: 62001

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  2. 62002 LCMS Vitamin D Mobile Phase B
    Mobile Phase B
    Order no.: 62002

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  3. 62003 LCMS Vitamin D Precipitation Reagent
    Precipitation Reagent
    Order no.: 62003

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  4. 62004 LCMS Vitamin D Internal Standard
    Internal Standard
    Order no.: 62004

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  5. 62009 LCMS rinsing solution vitamin D3 vitamin D2 serum plasma
    Rinsing Solution
    Order no.: 62009

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  6. 96 Well Filter Plates
    96 Well Filter Plates
    Order no.: 62057
    Details
  7. 96 Well Collection Plate
    Collection Plates
    Order no.: 62058
    Details
  8. 62100 LCMS Vitamin D Analytical Column
    Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Order no.: 62100

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  9. 62110 LCMS Vitamin D Trap Column
    Trap Column
    Order no.: 62110

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  1. 0221 0222 0223 0256 Vitamin D Serum Controls
    Details
  1. PEEK-encased Prefilter, 2 µm
    Details
  2. 62015 LCMS tuning mix vitamin D3 vitamin D2 serum plasma
    Tuning Mix 25-OH-Vitamin D3/D2
    Order no.: 62015

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  3. 62100 LCMS Vitamin D Analytical Column
    Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Order no.: 62100

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  4. 62110 LCMS Vitamin D Trap Column
    Trap Column
    Order no.: 62110

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  5. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
As a customer please login or register to gain full access.