Malondialdehyde in Serum/Plasma - HPLC

Order No.: 67000, for 100 tests
Parameters:
Malondialdehyde

The marker of lipid peroxidation
Trouble-free analysis
Reliable results

Malondialdehyde

Clinical relevance

Excessive oxidative stress can lead to degenerative damages in the body, such as oxidation of lipids, proteins, and DNA. The body has multiple endogenous biomarkers that can provide information about its physical condition, such as β-carotene, coenzyme Q10, glutathione, vitamin C and malondialdehyde (MDA), of which an increase in serum or plasma is a sign of oxidative stress.

MDA is an oxidation product of polyunsaturated fatty acids, and thus the laboratory diagnostic marker used for lipid peroxidation. Recent clinical studies show that the MDA concentration is elevated in patients with cystic fibrosis and other chronic respiratory diseases. The additional determination of the MDA level together with other parameters provides useful information about the degree of oxidative stress to optimise the therapy.

 

Product advantages

  • The marker for lipid peroxidation
  • Interference-free analysis
  • Reliable results

 

This Chromsystems kit allows the reliable chromatographic determination of malondialdehyde in one isocratic HPLC run with fluorescence detection. Sample preparation is based on an effective protein precipitation step followed by derivatisation. The resulting fluorophore is specific and detectable even at very low concentrations.

More Information
Method of Analysis HPLC
Number of Tests 100
Limit of quantification 0.01 µmol/l
Linearity up to 10 µmol/l
Recovery 99 %
Intraassay CV ≤ 2.9 %
Interassay CV ≤ 8.8 %
Analysis Time < 5 min
Specimen Plasma/Serum
Pre-analytic Treatment Fresh plasma is used. Patient samples are stable up to 12 h at +2 to 8 °C and up to 4 weeks below -18 °C. Deep-freeze for transport.
Sample Preparation
  • Mix 100 µl plasma and 500 µl Precipitation Reagent, mix for 10 s (vortex).

  • Centifuge 5 min at 16 000 x g.

  • Transfer 500 μl of the supernatant into a labelled derivatisation vial.

  • Add 100 μl Derivatisation Reagent and mix briefly.

  • Incubate 60 min at 95 °C, and cool down immediately.

  • Add 500 μl Neutralisation Buffer mix briefly.

  • Inject 20 µl into the HPLC system.

Sample Stability The prepared samples are stable at room temperature up to 24 h, at +2 to +8 °C up to 3 days and below -18 °C up to 4 weeks.
Injection Volume 20 µl
Flow rate 1.0 ml/min
Column temperature ambient (~ 25 °C)
Wavelength EX 515 nm, EM 553 nm
Gradient isocratic
Additional Info Any isocratic HPLC system with fluorescence detector is suitable.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Malondialdehyde
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