Malondialdehyde in Serum/Plasma - HPLC
The marker of lipid peroxidation
Excessive oxidative stress can lead to degenerative damages in the body, such as oxidation of lipids, proteins, and DNA. The body has multiple endogenous biomarkers that can provide information about its physical condition, such as β-carotene, coenzyme Q10, glutathione, vitamin C and malondialdehyde (MDA), of which an increase in serum or plasma is a sign of oxidative stress.
MDA is an oxidation product of polyunsaturated fatty acids, and thus the laboratory diagnostic marker used for lipid peroxidation. Recent clinical studies show that the MDA concentration is elevated in patients with cystic fibrosis and other chronic respiratory diseases. The additional determination of the MDA level together with other parameters provides useful information about the degree of oxidative stress to optimise the therapy.
- The marker for lipid peroxidation
- Interference-free analysis
- Reliable results
This Chromsystems kit allows the reliable chromatographic determination of malondialdehyde in one isocratic HPLC run with fluorescence detection. Sample preparation is based on an effective protein precipitation step followed by derivatisation. The resulting fluorophore is specific and detectable even at very low concentrations.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||0.01 µmol/l|
|Linearity||up to 10 µmol/l|
|Intraassay||CV ≤ 2.9 %|
|Interassay||CV ≤ 8.8 %|
|Analysis Time||< 5 min|
|Pre-analytic Treatment||Fresh plasma is used. Patient samples are stable up to 12 h at +2 to 8 °C and up to 4 weeks below -18 °C. Deep-freeze for transport.|
|Sample Stability||The prepared samples are stable at room temperature up to 24 h, at +2 to +8 °C up to 3 days and below -18 °C up to 4 weeks.|
|Injection Volume||20 µl|
|Flow rate||1.0 ml/min|
|Column temperature||ambient (~ 25 °C)|
|Wavelength||EX 515 nm, EM 553 nm|
|Additional Info||Any isocratic HPLC system with fluorescence detector is suitable.|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|