Crosslinks in Urine - HPLC
Hydrolysis resistant internal standard
Easy sample preparation
Accurate and reliable calibration
Deoxypyridinoline (DPD)
Pyridinoline (PYD)
Clinical relevance
Pyridinoline (PYD) and deoxypyridinoline (DPD) are produced to provide structural stability in the form of crosslinks when collagen fibrils are created. In pathological bone degeneration, the concentrations of PYD and DPD increase in the urine. This is the case with osteoporosis or various bone cancers, for example. Thus PYD and DPD are direct biomarkers for diagnosing post-menopausal osteoporosis and monitoring therapy after intake of drugs. A significant drop in crosslinks in the urine can be observed after just a few months of successful treatment. Furthermore, crosslink concentrations in the urine are subject to ongoing monitoring in patients at greater risk of fracture, so that suitable measures can be taken right away if the levels are already high.
Product advantages
- Hydrolysis-resistant internal standard
- Easy sample preparation
- Accurate and reliable calibration
This kit allows simple and reliable simultaneous quantification of pyridinoline (PYD) and deoxypyridinoline (DPD) in urine using a simple isocratic HPLC system. Chromatographic separation is performed at an RP column with subsequent fluorescence detection. The use of an acidic hydrolysis-resistant internal standard maximises the precision and reliability of the determination. Interfering fluorophores are removed using solid phase extraction, and then PYD, DPD, and internal standard are eluted in one run.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Limit of quantification | Pyridinoline: 15 pmol/ml |
| Linearity | Pyridinoline: up to 3200 pmol/ml |
| Recovery | Pyridinoline: 95.5 % (CV: 1.4 %); Deoxypyridinoline: 93.6 % (CV: 2.0 %) |
| Intraassay | Pyridinoline: CV = 3.8 % |
| Interassay | Pyridinoline: CV = 5.1 % |
| Analysis Time | < 15 min |
| Specimen | Spontaneous urine or collected for a period |
| Pre-analytic Treatment | Patient samples can be transported at room temperature. For further storage (up to 7 days), storage at +4 °C is recommended. For longer periods, samples should be stored below -18 °C. Protect against incident light! |
| Sample Preparation |
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| Sample Stability | The prepared samples are stable for 2 days at room temperature and for 4 days at +2 to +8 °C |
| Injection Volume | 50 µl |
| Flow rate | 1.2 ml/min |
| Column temperature | ambient (~ 25 °C) |
| Wavelength | EX 290 nm |
| Gradient | isocratic |
| Additional Info | Any isocratic HPLC system with fluorescence detector is suitable. |
| Please note | The product information provided shows typical values as an example only. Please review the information provided in instruction manual and product information leaflets. |
| Parameter | Deoxypyridinoline (DPD), Pyridinoline (PYD) |
