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MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

Order No.: 62000/1000/F, for 1000 tests
Parameters:
25-OH-Vitamin D2, 25-OH-Vitamin D3

For sample preparation with 96 Well Filter Plates

Assay validated according to IVDR (=> Declaration of Conformity)

main product photo

25-OH-Vitamin D2

25-OH-Vitamin D3

MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma by LC-MS/MS

Kit for 1000 tests, including Analytical Column, Trap Column, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls
For sample preparation with 96 Well Filter Plates

 

Clinical relevance

This assay allows the quantitative determination of 25-hydroxycholecalciferol (25-OH-vitamin D3) and 25-hydroxyergocalciferol (25-OH-vitamin D2) in human plasma or serum samples.

It is intended to be used for screening and/or monitoring of 25-OH-vitamin D3/D2 levels where indicated:

  • in patients with suspected vitamin D deficiency,
  • in patients with suspected vitamin D insufficiency, or
  • in patients with suspected vitamin D excess, and/or
  • in patients under vitamin D supplementation therapy.

The test kit is further intended to be used as an aid to diagnosis of diseases for which determination of vitamin D levels is indicated.

 

Assay characteristics

Manual sample preparation is limited to simple and effective protein precipitation. A trap column concentrates the analytes and separates interfering substances. A two-position-six-port-valve connects the trap column to an HPLC column where the chromatographic separation takes place. The use of atmospheric pressure chemical ionisation (APCI) and a deuterated internal standard assures precision and robustness and minimises ion suppression effects.

This kit does not separate 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2 from 25-OH-vitamin D3 and 25-OH-vitamin D2, respectively, and should therefore only be used to analyse samples that contain none or just negligible amounts of these metabolites.
We recommend the use of the assay MassChrom® 25-OH-Vitamin D3/D2 and 3-epi-25-OH-Vitamin D3 in Serum/Plasma (order no. 62062) for chromatographic separation of the diastereomers and quantitation of 3-epi-25-OH-vitamin D3 to ensure correct assessment of the vitamin D status in patient samples that might contain 3-epi-25-OH-vitamin D3/D2.

Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Number of Tests 1000
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation

25-OH-Vitamin D3: 1.0 µg/l – 250 µg/l
25-OH-Vitamin D2: 1.0 µg/l – 250 µg/l

Different systems might show different performance data.
Specimen Serum/Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

Sample preparation with 96 well filter plates:

  • Pipette 200 µl Internal Standard into 96 well filter plate.
  • Add 25 µl Precipitation Reagent.
  • Add 100 µl sample/calibrator/MassCheck® control and shake 10 min.
  • Place 96 well filter plate on collection plate.
  • Centrifuge 5 min at 2000 x g.
  • Seal collection plate and transfer to autosampler.
  • Inject ≤ 50 µl eluate into the LC-MS/MS system
Run Time 5.0 or 6.0 min (depending on gradient system)
Injection Volume ≤ 50 µl (mass spectrometer dependent)
Column Temperature Analytical Column and Trap Column: +20 to +25 °C
Ionisation APCI positive
MS/MS Mode MRM
Parameters 25-OH-Vitamin D2, 25-OH-Vitamin D3
The following components are included in the kit:
  1. 62001 LCMS Vitamin D Mobile Phase A
    Mobile Phase A
    Order no.: 62001

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  2. 62002 LCMS Vitamin D Mobile Phase B
    Mobile Phase B
    Order no.: 62002

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  3. 62003 LCMS Vitamin D Precipitation Reagent
    Precipitation Reagent
    Order no.: 62003

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  4. 62004 LCMS Vitamin D Internal Standard
    Internal Standard
    Order no.: 62004

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  5. 62009 LCMS rinsing solution vitamin D3 vitamin D2 serum plasma
    Rinsing Solution
    Order no.: 62009

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  6. 96 Well Filter Plates
    96 Well Filter Plates
    Order no.: 62057
    Details
  7. 96 Well Collection Plate
    Collection Plates
    Order no.: 62058
    Details
  8. Pierceable Adhesive Seals for 96 Well Plates
    Pierceable Adhesive Seals for 96 Well Plates
    Order no.: 62059
    Details
  9. 62100 LCMS Vitamin D Analytical Column
    Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Order no.: 62100

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  10. 62110 LCMS Vitamin D Trap Column
    Trap Column
    Order no.: 62110
    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma – LC-MS/MS
    Details
  11. 62028 3PLUS1 Calibrator Vitamin D
    3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2
    Order no.: 62028
    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Details
  12. 0221 0222 0223 0256 Vitamin D Serum Controls
    MassCheck® 25-OH-Vitamin D3/D2 Serum Controls
    Order no.: 0221/0222/0223/0256

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS

    Details
The following products are not included in the kit but are required for the application of the method:
  1. 62015 LCMS tuning mix vitamin D3 vitamin D2 serum plasma
    Tuning Mix 25-OH-Vitamin D3/D2
    Order no.: 62015

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  1. 62028 3PLUS1 Calibrator Vitamin D
    3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2
    Order no.: 62028
    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Details
  1. 0221 0222 0223 0256 Vitamin D Serum Controls
    MassCheck® 25-OH-Vitamin D3/D2 Serum Controls
    Order no.: 0221/0222/0223/0256

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS

    Details
  1. 62100 LCMS Vitamin D Analytical Column
    Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Order no.: 62100

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  4. Stainless Steel Prefilter - 0.5 µm
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details
As a customer please login or register to gain full access.

25-OH-Vitamin D2

25-OH-Vitamin D3

MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma by LC-MS/MS

Kit for 1000 tests, including Analytical Column, Trap Column, 3PLUS1® Multilevel Calibrator Set and MassCheck® Controls
For sample preparation with 96 Well Filter Plates

 

Clinical relevance

This assay allows the quantitative determination of 25-hydroxycholecalciferol (25-OH-vitamin D3) and 25-hydroxyergocalciferol (25-OH-vitamin D2) in human plasma or serum samples.

It is intended to be used for screening and/or monitoring of 25-OH-vitamin D3/D2 levels where indicated:

  • in patients with suspected vitamin D deficiency,
  • in patients with suspected vitamin D insufficiency, or
  • in patients with suspected vitamin D excess, and/or
  • in patients under vitamin D supplementation therapy.

The test kit is further intended to be used as an aid to diagnosis of diseases for which determination of vitamin D levels is indicated.

 

Assay characteristics

Manual sample preparation is limited to simple and effective protein precipitation. A trap column concentrates the analytes and separates interfering substances. A two-position-six-port-valve connects the trap column to an HPLC column where the chromatographic separation takes place. The use of atmospheric pressure chemical ionisation (APCI) and a deuterated internal standard assures precision and robustness and minimises ion suppression effects.

This kit does not separate 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2 from 25-OH-vitamin D3 and 25-OH-vitamin D2, respectively, and should therefore only be used to analyse samples that contain none or just negligible amounts of these metabolites.
We recommend the use of the assay MassChrom® 25-OH-Vitamin D3/D2 and 3-epi-25-OH-Vitamin D3 in Serum/Plasma (order no. 62062) for chromatographic separation of the diastereomers and quantitation of 3-epi-25-OH-vitamin D3 to ensure correct assessment of the vitamin D status in patient samples that might contain 3-epi-25-OH-vitamin D3/D2.

Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.

More Information
Method of Analysis LC-MS/MS
Number of Tests 1000
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in Appendices II and III of the instruction manual.
Lower and Upper Limit of Quantitation

25-OH-Vitamin D3: 1.0 µg/l – 250 µg/l
25-OH-Vitamin D2: 1.0 µg/l – 250 µg/l

Different systems might show different performance data.
Specimen Serum/Plasma
Sample Preparation

The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.

Sample preparation with 96 well filter plates:

  • Pipette 200 µl Internal Standard into 96 well filter plate.
  • Add 25 µl Precipitation Reagent.
  • Add 100 µl sample/calibrator/MassCheck® control and shake 10 min.
  • Place 96 well filter plate on collection plate.
  • Centrifuge 5 min at 2000 x g.
  • Seal collection plate and transfer to autosampler.
  • Inject ≤ 50 µl eluate into the LC-MS/MS system
Run Time 5.0 or 6.0 min (depending on gradient system)
Injection Volume ≤ 50 µl (mass spectrometer dependent)
Column Temperature Analytical Column and Trap Column: +20 to +25 °C
Ionisation APCI positive
MS/MS Mode MRM
Parameters 25-OH-Vitamin D2, 25-OH-Vitamin D3
The following components are included in the kit:
  1. 62001 LCMS Vitamin D Mobile Phase A
    Mobile Phase A
    Order no.: 62001

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  2. 62002 LCMS Vitamin D Mobile Phase B
    Mobile Phase B
    Order no.: 62002

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  3. 62003 LCMS Vitamin D Precipitation Reagent
    Precipitation Reagent
    Order no.: 62003

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  4. 62004 LCMS Vitamin D Internal Standard
    Internal Standard
    Order no.: 62004

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  5. 62009 LCMS rinsing solution vitamin D3 vitamin D2 serum plasma
    Rinsing Solution
    Order no.: 62009

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma

    Details
  6. 96 Well Filter Plates
    96 Well Filter Plates
    Order no.: 62057
    Details
  7. 96 Well Collection Plate
    Collection Plates
    Order no.: 62058
    Details
  8. Pierceable Adhesive Seals for 96 Well Plates
    Pierceable Adhesive Seals for 96 Well Plates
    Order no.: 62059
    Details
  9. 62100 LCMS Vitamin D Analytical Column
    Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Order no.: 62100

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  10. 62110 LCMS Vitamin D Trap Column
    Trap Column
    Order no.: 62110
    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma – LC-MS/MS
    Details
  11. 62028 3PLUS1 Calibrator Vitamin D
    3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2
    Order no.: 62028
    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Details
  12. 0221 0222 0223 0256 Vitamin D Serum Controls
    MassCheck® 25-OH-Vitamin D3/D2 Serum Controls
    Order no.: 0221/0222/0223/0256

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS

    Details
The following products are not included in the kit but are required for the application of the method:
  1. 62015 LCMS tuning mix vitamin D3 vitamin D2 serum plasma
    Tuning Mix 25-OH-Vitamin D3/D2
    Order no.: 62015

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  1. 62028 3PLUS1 Calibrator Vitamin D
    3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2
    Order no.: 62028
    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Details
  1. 0221 0222 0223 0256 Vitamin D Serum Controls
    MassCheck® 25-OH-Vitamin D3/D2 Serum Controls
    Order no.: 0221/0222/0223/0256

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC/MS-MS

    Details
  1. 62100 LCMS Vitamin D Analytical Column
    Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
    Order no.: 62100

    MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS

    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  4. Stainless Steel Prefilter - 0.5 µm
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details
As a customer please login or register to gain full access.