Vitamin Profiling

Choose from a range of complete assays for vitamin profiling. Calibrators and controls are also available separately. 

  • HPLC/UHPLC and LC-MS/MS assays 
  • Reliable methods with fast sample preparation
  • Calibrators and controls available separately 

Assays Available for Vitamin Profiling

Assays for the Combined Analysis of Vitamin A and E in Serum/Plasma

Order No. Method Number of tests Analysis time Characteristics
34000 HPLC 100 9 min Standard method
34000/Premix HPLC 100 9 min One-step sample prep / pre-mixed tubes
34900/UHPLC UHPLC 1.000 3.5 min One-step sample prep / pre-mixed tubes
Short analysis time, high sample throughput
34700/F HPLC 5 x 96 9 min HPLC method, automated
34900/F UHPLC 10 x 96 3.5 min UHPLC method, automated. Short analysis time, high sample throughput

Vitamin A (retinol) is essential for the formation of rhodopsin, for bone metabolism, and for the synthesis of steroid hormones. Deficiency of vitamin A leads to night blindness, dry skin, and loss of hair. Vitamin E (α-tocopherol) is a potent antioxidant that protects LDL cholesterol and cellular membranes from lipid peroxidation, which mainly occurs as a result of increased oxidative stress in the organism.

Chromsystems offers CE-IVD validated complete assays as well as calibrators and controls. Plus there are solutions available for any requirements:

  •    Standard method or one-step sample preparation with Pre-mixed Tubes
  •    UHPLC methods for short analysis time and high sample throughput
  •    Automated methods

Assays for the Analysis of Vitamin C in Plasma/Serum

Order No. Method Number of tests Analysis time Characteristics
65065 HPLC 100 5 min Standard method
65765/F HPLC 480 5 min Automated method with 96 well filter plates

Vitamin C (ascorbic acid) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

Assay for the Analysis of β-Carotene in Serum/Plasma

Order No. Method Number of tests Analysis time Characteristics
32000 HPLC 100  < 10 min Fast and easy sample prep

β-Carotene (Provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products. In humans, all-trans-β-carotene is functionally and quantitatively the most important of about 20 carotenoids present in the blood plasma. This assay assures reliable separation of α-carotene, cis-β-carotene and all-trans-β-carotene as well as the carotenoids cryptoxanthin and lycopene.

Research-Use Products*

Assays for the Combined Analysis of Vitamin B1 and B6 in Whole Blood and Plasma/Serum*

Order No. Method  Specimen Number of tests Analysis time Characteristics
52052/RUO HPLC Whole Blood
B6 also Plasma
100 < 9 min Classic method
52052/Premix/RUO HPLC Whole Blood
B6 also Plasma
100 < 9 min Simplified method with pre-mixed tubes
52952/UHPLC/RUO UHPLC Whole Blood
B6 also Plasma
1000 < 4 min Simplified method with pre-mixed tubes,
high sample throughput

Assay for the Single Analysis of Vitamin B1 in Whole Blood*

Order No. Method  Specimen Number of tests Analysis time Characteristics
35000/RUO HPLC Whole Blood 100  < 6 min No post-column derivatisation needed

Assays for the Single Analysis of Vitamin B6 in Whole Blood and Plasma/Serum*

Order No. Method  Specimen Number of tests Analysis time Characteristics
31000/S/RUO HPLC Plasma/Serum 100 < 8 min Fast and easy sample prep

Assay for the Analysis of Vitamin B2 in Whole Blood*

Order No. Method Number of tests Analysis time Characteristics
37000/RUO HPLC 100  < 9 min Simultaneous determination of riboflavin, FMN and FAD

Assays for the Analysis of Vitamin D3/D2 in Plasma/Serum*

Order No. Method Number of tests Analysis time Characteristics
62000/RUO
62000/1000/RUO
LC-MS/MS 200
1000
5 min With online sample preparation

Assay for the Analysis of Methylmalonic Acid in Serum/Plasma and Urine*

Order No. Method Number of tests Analysis time Characteristics
64000/RUO LC-MS/MS 400 3.5 min Clean-up tubes for fast processing of serum/plasma samples or
direct injection of urine samples after dilution
64000 Urine Upgrade Set/RUO LC-MS/MS 200 3.5 min Upgrade set for methylmalonic acid in urine

* For Research Use Only. Not for use in diagnostic procedures.