Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
Each analyte safeguarded by a specific internal standard
No post-column derivatisation required
One sample preparation and one run for both analytes
Simplified sample prep with Pre-mixed Tubes
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
This Chromsystems assay facilitates the combined analysis of vitamin B1 in whole blood and vitamin B6 in whole blood and plasma research samples. The efficient sample preparation with Pre-mixed Tubes processes different matrices, thus whole blood research samples can easily be measured in the same sequence with plasma research samples. Vitamin molecules are derivatised during sample preparation which renders the common post-column derivatisation unnecessary. The separation takes place with a binary gradient. Both analytes are safeguarded by specifically developed internal standards. Quality controls are also available separately.
Method of Analysis | HPLC |
---|---|
Number of Tests | 100 |
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
Specimen | Whole Blood and Whole Blood/Plasma |
Sample Preparation |
|
Run Time | 7 - 9 min |
Injection Volume | 25-50 µl |
Flow Rate | 1.5 - 2.3 ml/min |
Column Temperature | ambient (~ 25 °C) |
Gradient | Gradient profile: specific binary gradients for whole blood and plasma |
Wavelengths | Start EX 320 nm, EM 415 nm, |
Additional Info | For the HPLC analysis of vitamins B1 and B6 in whole blood or plasma every common HPLC system with binary pump and programmable fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
-
Mobile Phase AOrder no.: 52001/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Mobile Phase BOrder no.: 52022/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Internal StandardOrder no.: 52044/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Precipitation ReagentOrder no.: 52005/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Neutralisation ReagentOrder no.: 52006/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Pre-mixed Neutralisation TubesOrder no.: 52906/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 1Order no.: 52007/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 2Order no.: 52008/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Precolumn Cartridge 4/10Order no.: 18052/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
This Chromsystems assay facilitates the combined analysis of vitamin B1 in whole blood and vitamin B6 in whole blood and plasma research samples. The efficient sample preparation with Pre-mixed Tubes processes different matrices, thus whole blood research samples can easily be measured in the same sequence with plasma research samples. Vitamin molecules are derivatised during sample preparation which renders the common post-column derivatisation unnecessary. The separation takes place with a binary gradient. Both analytes are safeguarded by specifically developed internal standards. Quality controls are also available separately.
Method of Analysis | HPLC |
---|---|
Number of Tests | 100 |
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
Specimen | Whole Blood and Whole Blood/Plasma |
Sample Preparation |
|
Run Time | 7 - 9 min |
Injection Volume | 25-50 µl |
Flow Rate | 1.5 - 2.3 ml/min |
Column Temperature | ambient (~ 25 °C) |
Gradient | Gradient profile: specific binary gradients for whole blood and plasma |
Wavelengths | Start EX 320 nm, EM 415 nm, |
Additional Info | For the HPLC analysis of vitamins B1 and B6 in whole blood or plasma every common HPLC system with binary pump and programmable fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
-
Mobile Phase AOrder no.: 52001/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Mobile Phase BOrder no.: 52022/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Internal StandardOrder no.: 52044/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Precipitation ReagentOrder no.: 52005/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Neutralisation ReagentOrder no.: 52006/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Pre-mixed Neutralisation TubesOrder no.: 52906/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 1Order no.: 52007/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 2Order no.: 52008/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Precolumn Cartridge 4/10Order no.: 18052/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC