Amino Acid Analysis (AAA)
You can quantify 48 amino acids in research samples of plasma/serum or quantify 52 amino acids and creatinine in research samples of urine in one run.
- One chromatographic platform for plasma/serum and urine
- Fast sample preparation without derivatisation
- Short run times
- Excellent separation of all isobaric compounds
- Each analyte with its own internal standard
- Reaction vials or 96 deep well plates
Assays for Quantitative Amino Acid Analysis
MassChrom® Amino Acid Analysis in Plasma/Serum*
Order no. | Method | Number of tests |
Characteristics |
75111/RUO | LC-MS/MS | 3 x 96 | Assay with reaction vials |
75111/DWP/RUO | LC-MS/MS | 3 x 96 | Assay with 96 deep well plates |
One Assay - Two Test Modes
You can either measure 48 amino acids within 20 minutes, or alternatively determine the PKU/MSUD panel within 8 minutes.The analytes measured with both, the Full Panel and the PKU/MSUD Panel, are leucine, allo-isoleucine, isoleucine, methionine, phenylalanine, tyrosine and valine. Both panels use the identical chromatographic set-up and sample preparation.
MassChrom® Amino Acid Analysis in Urine*
Order no. | Method | Number of tests |
Characteristics |
75222/RUO | LC-MS/MS | 3 x 96 | Assay with reaction vials |
75222/DWP/RUO | LC-MS/MS | 3 x 96 | Assay with 96 deep well plates |
52 Amino Acids and Creatinine in One Run
This assay allows the quantitative determination of 52 amino acids/metabolites and creatinine by LC-MS/MS within 20 minutes. Creatinine is also measured within the same run, which is used for the normalisation of the urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination and subsequent dilution of the sample. The assay can be ideally combined with our plasma assay without needing to change the chromatographic set-up.
All Parameters at a Glance
Acetyltyrosine Adenosylhomocysteine Alanine β-Alanine α-Aminoadipic acid α-Aminobutyric acid β-Aminoisobutyric acid γ-Aminobutyric acid Anserine Arginine Argininosuccinic acid Asparagine Aspartic acid Carnosine |
Citrulline Homocitrulline Cystathionine Cystine Cysteine-S-sulfate Homocystine Ethanolamine Phosphoethanolamine Glutamic acid Glutamine Glycine Histidine 1-Methylhistidine 3-Methylhistidine |
Kynurenine** Hydroxykynurenine** Leucine Allo-Isoleucine Isoleucine Lysine Hydroxylysine Methionine Ornithine Oxoproline/Pyroglutamic acid** Phenylacetylglutamine** Phenylalanine Phosphoserine Pipecolic acid |
Proline 4-Hydroxyproline Saccharopine Sarcosine Serine Taurine Threonine Tryptophan Tyrosine Valine Creatinine** |
** These additional 5 parameters are included in the urine assay.
Dr Rachel Carling, Scientific Director for Viapath, reports on a study that compares the current gold standard in quantitative amino acid analysis (AAA) - ion exchange chromatography (IEC) with LC-MS/MS.
One Assay - Two Test Modes
Full Panel
Acetyltyrosine, Adenosylhomocysteine, Alanine, β-Alanine, a-Aminoadipic acid, a-Aminobutyric acid, β-Aminoisobutyric acid, y-Aminobutyric acid, Anserine, Arginine, Argininosuccinic acid, Asparagine, Aspartic acid, Carnosine, Citrulline, Homocitrulline, Cystathionine, Cysteine S-sulfate, Cystine, Homocystine, Ethanolamine, Phosphoethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, 1-Methylhistidine, 3-Methylhistidine, Leucine*, Isoleucine*, Allo-Isoleucine*, Lysine, Hydroxylysine, Methionine*, Ornithine, Phenylalanine*, Phosphoserine, Pipecolic acid, Proline, 4-Hydroxyproline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine*, Valine*
Specific Panel of 7 Amino Acids
Leucine*, Isoleucine*, Allo-Isoleucine*, Methionine*, Phenylalanine*, Tyrosine*, Valine*
*The 7 analytes highlighted in red can be measured with both the full panel and the specific panel.