Amino Acid Analysis - ChromsystemsAmino Acid Analysis - Chromsystems

Amino Acid Analysis (AAA)

You can quantify 48 amino acids in research samples of plasma/serum or quantify 52 amino acids and creatinine in research samples of urine in one run.

  • One chromatographic platform for plasma/serum and urine
  • Fast sample preparation without derivatisation
  • Short run times
  • Excellent separation of all isobaric compounds
  • Each analyte with its own internal standard
  • Reaction vials or 96 deep well plates

Assays for Quantitative Amino Acid Analysis

MassChrom® Amino Acid Analysis in Plasma/Serum*

Order no. Method Number of tests
Characteristics
75111/RUO LC-MS/MS 3 x 96 Assay with reaction vials
75111/DWP/RUO LC-MS/MS 3 x 96 Assay with 96 deep well plates

 

One Assay - Two Test Modes

You can either measure 48 amino acids within 20 minutes, or alternatively determine the PKU/MSUD panel within 8 minutes.The analytes measured with both, the Full Panel and the PKU/MSUD Panel, are leucine, allo-isoleucine, isoleucine, methionine, phenylalanine, tyrosine and valine. Both panels use the identical chromatographic set-up and sample preparation.

MassChrom® Amino Acid Analysis in Urine*

Order no. Method Number of tests
Characteristics
75222/RUO LC-MS/MS 3 x 96 Assay with reaction vials
75222/DWP/RUO LC-MS/MS 3 x 96 Assay with 96 deep well plates

 

52 Amino Acids and Creatinine in One Run

This assay allows the quantitative determination of 52 amino acids/metabolites and creatinine by LC-MS/MS within 20 minutes. Creatinine is also measured within the same run, which is used for the normalisation of the urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination and subsequent dilution of the sample. The assay can be ideally combined with our plasma assay without needing to change the chromatographic set-up.


All Parameters at a Glance

Acetyltyrosine
Adenosylhomocysteine
Alanine
β-Alanine
α-Aminoadipic acid
α-Aminobutyric acid
β-Aminoisobutyric acid
γ-Aminobutyric acid
Anserine
Arginine
Argininosuccinic acid
Asparagine
Aspartic acid
Carnosine
Citrulline
Homocitrulline
Cystathionine
Cystine
Cysteine-S-sulfate
Homocystine
Ethanolamine
Phosphoethanolamine
Glutamic acid
Glutamine
Glycine
Histidine
1-Methylhistidine
3-Methylhistidine
Kynurenine**
Hydroxykynurenine**
Leucine
Allo-Isoleucine
Isoleucine
Lysine
Hydroxylysine
Methionine
Ornithine
Oxoproline/Pyroglutamic acid**
Phenylacetylglutamine**
Phenylalanine
Phosphoserine
Pipecolic acid
Proline
4-Hydroxyproline
Saccharopine
Sarcosine
Serine
Taurine
Threonine
Tryptophan
Tyrosine
Valine

Creatinine**

** These additional 5 parameters are included in the urine assay.


Dr Rachel Carling, Scientific Director for Viapath, reports on a study that compares the current gold standard in quantitative amino acid analysis (AAA) - ion exchange chromatography (IEC) with LC-MS/MS.


One Assay - Two Test Modes

Amino Acid Analysis - Full PKU-MSUD-Panel - ChromsystemsAmino Acid Analysis - Full PKU-MSUD-Panel - Chromsystems

Full Panel

Acetyltyrosine, Adenosylhomocysteine, Alanine, β-Alanine, a-Aminoadipic acid, a-Aminobutyric acid, β-Aminoisobutyric acid, y-Aminobutyric acid, Anserine, Arginine, Argininosuccinic acid, Asparagine, Aspartic acid, Carnosine, Citrulline, Homocitrulline, Cystathionine, Cysteine S-sulfate, Cystine, Homocystine, Ethanolamine, Phosphoethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, 1-Methylhistidine, 3-Methylhistidine, Leucine*, Isoleucine*, Allo-Isoleucine*, Lysine, Hydroxylysine, Methionine*, Ornithine, Phenylalanine*, Phosphoserine, Pipecolic acid, Proline, 4-Hydroxyproline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine*, Valine*

Amino Acid Analysis - PKU-MSUD-Panel - ChromsystemsAmino Acid Analysis - PKU-MSUD-Panel - Chromsystems

Specific Panel of 7 Amino Acids 

Leucine*, Isoleucine*, Allo-Isoleucine*, Methionine*, Phenylalanine*, Tyrosine*, Valine*

*The 7 analytes highlighted in red can be measured with both the full panel and the specific panel.


* For Research Use Only. Not for use in diagnostic procedures.