Amino Acid Analysis (AAA)

You can quantify 48 amino acids in research samples of plasma/serum or quantify 52 amino acids and creatinine in research samples of urine in one run.

• One chromatographic platform for plasma/serum and urine
• Fast sample preparation without derivatisation
• Short run times
• Excellent separation of all isobaric compounds
• Each analyte with its own internal standard
• Reaction vials or 96 deep well plates

One Assay - Two Test Modes

You can either measure 48 amino acids in 20 minutes, or alternatively determine the PKU/MSUD panel within 8 minutes.The analytes measured with both, the Full Panel and the PKU/MSUD Panel, are leucine, allo-isoleucine, isoleucine, methionine, phenylalanine, tyrosine and valine. Both panels use the identical chromatographic set-up and sample preparation.

52 Amino Acids and Creatinine in One Run

This assay allows the quantitative determination of 52 amino acids/metabolites and creatinine by LC-MS/MS in 20 minutes. Creatinine is also measured within the same run, which is used for the normalisation of the urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination and subsequent dilution of the sample. The assay can be ideally combined with our plasma assay without needing to change the chromatographic set-up.

Educational Webinar: Challenging the Status Quo of Amino Acid Analysis in Plasma

Dr Rachel Carling, Scientific Director for Viapath, reports on a study that compares the current gold standard in quantitative amino acid analysis (AAA) - ion exchange chromatography (IEC) with LC-MS/MS.

Acetyltyrosine, Adenosylhomocysteine, Alanine, β-Alanine, a-Aminoadipic acid, a-Aminobutyric acid, β-Aminoisobutyric acid, y-Aminobutyric acid, Anserine, Arginine, Argininosuccinic acid, Asparagine, Aspartic acid, Carnosine, Citrulline, Homocitrulline, Cystathionine, Cysteine S-sulfate, Cystine, Homocystine, Ethanolamine, Phosphoethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, 1-Methylhistidine, 3-Methylhistidine, Leucine*, Isoleucine*, Allo-Isoleucine*, Lysine, Hydroxylysine, Methionine*, Ornithine, Phenylalanine*, Phosphoserine, Pipecolic acid, Proline, 4-Hydroxyproline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine*, Valine*

Leucine*, Isoleucine*, Allo-Isoleucine*, Methionine*, Phenylalanine*, Tyrosine*, Valine*