MassChrom® Amino Acid Analysis in Urine with Reaction Vials - LC-MS/MS
Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
Creatinine normalisation within the same run
Fast sample preparation without derivatisation
Short run times
Separation of all isobaric analytes
Each analyte with its own internal standard
One chromatographic platform for plasma/serum and urine
Acetyltyrosine Adenosylhomocysteine Alanine β-Alanine α-Aminoadipic acid α-Aminobutyric acid β-Aminoisobutyric acid γ-Aminobutyric acid Anserine Arginine Argininosuccinic acid Asparagine Aspartic acid Carnosine |
Citrulline Homocitrulline Cystathionine Cystine Cysteine S-sulfate Homocystine Ethanolamine Phosphoethanolamine Glutamic acid Glutamine Glycine Histidine 1-Methylhistidine 3-Methylhistidine |
Kynurenine Hydroxykynurenine Leucine Allo-Isoleucine Isoleucine Lysine Hydroxylysine Methionine Ornithine Oxoproline / Pyroglutamic acid Phenylacetylglutamine Phenylalanine Phosphoserine Pipecolic acid |
Proline 4-Hydroxyproline Saccharopine Sarcosine Serine Taurine Threonine Tryptophan Tyrosine Valine Creatinine * |
* With this assay the creatinine level in urine can be determined within the same run!
MassChrom® Amino Acid Analysis in Urine enables the quantitative determination of 52 amino acids / metabolites by LC-MS/MS within 20 minutes. Creatinine is also measured within the same run and is used for the normalization of urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination step and the subsequent dilution of research samples. The assay can be combined with the plasma assay (75111/RUO) without needing to change the chromatographic set-up. The chromatography is also optimised to effectively separate all isobaric analytes. Derivatisation of research samples is not required, therefore, a simplified sample preparation can be performed faster and error-prone steps can be omitted for an increased accuracy of results and higher robustness of the method. Each of the 53 analytes is safeguarded by its own internal standard, which ensures the maximum accuracy and reliability of the method by compensating any variations in recovery, matrix effects and ionisation effectiveness. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.
Product advantages
- Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
- Creatinine normalisation within the same run
- Fast sample preparation without derivatisation
- Short run times
- Separation of all isobaric analytes
- Each analyte with its own internal standard
- One chromatographic platform for plasma/serum and urine
Method of Analysis | LC-MS/MS |
---|---|
Number of Tests | 3 x 96 |
Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual. |
Specimen | Urine |
Sample Preparation |
|
Run Time | < 20 min |
Injection Volume | up to 5 µl |
Flow Rate | 0.3–1.8 ml/min |
Column Temperature | + 25°C |
Gradient | binary gradient, flow gradient |
Ionisation | ESI positive |
MS/MS Mode | MRM |
Parameters | α-Aminoadipic Acid, α-Aminobutyric Acid, β-Alanine, β-Aminoisobutyric Acid, γ-Aminobutyric Acid, 1-Methylhistidine, 3-Methylhistidine, 4-Hydroxyproline, Acetyltyrosine, Adenosylhomocysteine, Alanine, Allo-Isoleucine, Anserine, Arginine, Argininosuccinic Acid, Asparagine, Aspartic Acid, Carnosine, Citrulline, Creatinine, Cystathionine, Cysteine S-sulfate, Cystine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocitrulline, Homocystine, Hydroxykynurenine, Hydroxylysine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Oxoproline / Pyroglutamic acid, Phenylacetylglutamine, Phenylalanine, Phosphoserine, Pipecolic Acid, Proline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Valine |
-
MassChrom® Amino Acid Analysis - Urine SetOrder no.: 75211/RUO
MassChrom® Amino Acid Analysis in Urine with Reaction Vials
Consisting of reagents and lab equipment
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Tuning Mix 1 Amino Acid AnalysisOrder no.: 75015/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 2 Amino Acid AnalysisOrder no.: 75016/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 3 Amino Acid AnalysisOrder no.: 75017/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 4 Amino Acid AnalysisOrder no.: 75018/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 5 Amino Acid AnalysisOrder no.: 75019/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 6 Amino Acid Analysis in UrineOrder no.: 75220/RUOMassChrom® Amino Acid Analysis in Urine – LC-MS/MS
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
Acetyltyrosine Adenosylhomocysteine Alanine β-Alanine α-Aminoadipic acid α-Aminobutyric acid β-Aminoisobutyric acid γ-Aminobutyric acid Anserine Arginine Argininosuccinic acid Asparagine Aspartic acid Carnosine |
Citrulline Homocitrulline Cystathionine Cystine Cysteine S-sulfate Homocystine Ethanolamine Phosphoethanolamine Glutamic acid Glutamine Glycine Histidine 1-Methylhistidine 3-Methylhistidine |
Kynurenine Hydroxykynurenine Leucine Allo-Isoleucine Isoleucine Lysine Hydroxylysine Methionine Ornithine Oxoproline / Pyroglutamic acid Phenylacetylglutamine Phenylalanine Phosphoserine Pipecolic acid |
Proline 4-Hydroxyproline Saccharopine Sarcosine Serine Taurine Threonine Tryptophan Tyrosine Valine Creatinine * |
* With this assay the creatinine level in urine can be determined within the same run!
MassChrom® Amino Acid Analysis in Urine enables the quantitative determination of 52 amino acids / metabolites by LC-MS/MS within 20 minutes. Creatinine is also measured within the same run and is used for the normalization of urinary amino acid concentrations. This eliminates the need for the time-consuming photometric determination step and the subsequent dilution of research samples. The assay can be combined with the plasma assay (75111/RUO) without needing to change the chromatographic set-up. The chromatography is also optimised to effectively separate all isobaric analytes. Derivatisation of research samples is not required, therefore, a simplified sample preparation can be performed faster and error-prone steps can be omitted for an increased accuracy of results and higher robustness of the method. Each of the 53 analytes is safeguarded by its own internal standard, which ensures the maximum accuracy and reliability of the method by compensating any variations in recovery, matrix effects and ionisation effectiveness. The assay is available in 2 configurations, either with reaction vials or with 96 deep well plates.
Product advantages
- Quantitative amino acid analysis (AAA) of 52 analytes and creatinine
- Creatinine normalisation within the same run
- Fast sample preparation without derivatisation
- Short run times
- Separation of all isobaric analytes
- Each analyte with its own internal standard
- One chromatographic platform for plasma/serum and urine
Method of Analysis | LC-MS/MS |
---|---|
Number of Tests | 3 x 96 |
Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual. |
Specimen | Urine |
Sample Preparation |
|
Run Time | < 20 min |
Injection Volume | up to 5 µl |
Flow Rate | 0.3–1.8 ml/min |
Column Temperature | + 25°C |
Gradient | binary gradient, flow gradient |
Ionisation | ESI positive |
MS/MS Mode | MRM |
Parameters | α-Aminoadipic Acid, α-Aminobutyric Acid, β-Alanine, β-Aminoisobutyric Acid, γ-Aminobutyric Acid, 1-Methylhistidine, 3-Methylhistidine, 4-Hydroxyproline, Acetyltyrosine, Adenosylhomocysteine, Alanine, Allo-Isoleucine, Anserine, Arginine, Argininosuccinic Acid, Asparagine, Aspartic Acid, Carnosine, Citrulline, Creatinine, Cystathionine, Cysteine S-sulfate, Cystine, Ethanolamine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocitrulline, Homocystine, Hydroxykynurenine, Hydroxylysine, Isoleucine, Kynurenine, Leucine, Lysine, Methionine, Ornithine, Oxoproline / Pyroglutamic acid, Phenylacetylglutamine, Phenylalanine, Phosphoserine, Pipecolic Acid, Proline, Saccharopine, Sarcosine, Serine, Taurine, Threonine, Tryptophan, Tyrosine, Valine |
-
MassChrom® Amino Acid Analysis - Urine SetOrder no.: 75211/RUO
MassChrom® Amino Acid Analysis in Urine with Reaction Vials
Consisting of reagents and lab equipment
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS
-
Tuning Mix 1 Amino Acid AnalysisOrder no.: 75015/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 2 Amino Acid AnalysisOrder no.: 75016/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 3 Amino Acid AnalysisOrder no.: 75017/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 4 Amino Acid AnalysisOrder no.: 75018/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 5 Amino Acid AnalysisOrder no.: 75019/RUO
MassChrom® Amino Acid Analysis in Plasma/Serum - LC-MS/MS
-
Tuning Mix 6 Amino Acid Analysis in UrineOrder no.: 75220/RUOMassChrom® Amino Acid Analysis in Urine – LC-MS/MS
-
Analytical Column Amino Acid Analysis - LC-MS/MSOrder no.: 75100MassChrom® Amino Acid Analysis in Plasma/Serum or Urine - LC-MS/MS