Vitamin B2 in Whole Blood - HPLC

Order No.: 37000/RUO, for 100 tests
Parameters:
Flavin adenine dinucleotide (FAD), Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin)

Safe results by stabilisation of FAD
Simultaneous determination of FMN
Highly stable calibrators and controls

Flavin adenine dinucleotide (FAD)

Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin)

The present method allows the rapid and simple determination of riboflavin, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) in whole blood using an isocratic HPLC system. Due to their native fluorescence, the analytes can be detected directly, without derivatisation. Sample preparation is restricted to one effective protein precipitation step immediately after addition of the extraction buffer and subsequent stabilisation of the analytes. A dedicated HPLC column and a mobile phase, optimised for the specific separation, permit high performance in HPLC quantification.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Whole Blood
Sample Preparation
  • Pipette the following solutions into an amber reaction vial (light protected) and mix immediately for 30 s (vortex): 200 μl research sample/calibrator/control + 200 μl Extraction Buffer + 400 μl Precipitation Reagent.
  • Centrifuge for 10 minutes at a minimum of 9000 x g.
  • Pipette 100 μl of Neutralisation Buffer into an amber autosampler vial (light protected), then add 500 μl supernatant and mix well (vortex).
  • Inject 50 μl of this mixture into the HPLC system.
Run Time approx. 9 min
Injection Volume 50 µl
Flow Rate 1.2 ml/min
Column Temperature ambient (~20 °C)
Wavelengths

EX 465 nm

EM 525 nm

Additional Info For the analysis of vitamin B2 in whole blood every isocratic system with fluorescence detector is suitable.
Parameters Flavin adenine dinucleotide (FAD), Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:

Flavin adenine dinucleotide (FAD)

Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin)

The present method allows the rapid and simple determination of riboflavin, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) in whole blood using an isocratic HPLC system. Due to their native fluorescence, the analytes can be detected directly, without derivatisation. Sample preparation is restricted to one effective protein precipitation step immediately after addition of the extraction buffer and subsequent stabilisation of the analytes. A dedicated HPLC column and a mobile phase, optimised for the specific separation, permit high performance in HPLC quantification.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Specimen Whole Blood
Sample Preparation
  • Pipette the following solutions into an amber reaction vial (light protected) and mix immediately for 30 s (vortex): 200 μl research sample/calibrator/control + 200 μl Extraction Buffer + 400 μl Precipitation Reagent.
  • Centrifuge for 10 minutes at a minimum of 9000 x g.
  • Pipette 100 μl of Neutralisation Buffer into an amber autosampler vial (light protected), then add 500 μl supernatant and mix well (vortex).
  • Inject 50 μl of this mixture into the HPLC system.
Run Time approx. 9 min
Injection Volume 50 µl
Flow Rate 1.2 ml/min
Column Temperature ambient (~20 °C)
Wavelengths

EX 465 nm

EM 525 nm

Additional Info For the analysis of vitamin B2 in whole blood every isocratic system with fluorescence detector is suitable.
Parameters Flavin adenine dinucleotide (FAD), Flavin mononucleotide (FMN), Vitamin B2 (Riboflavin)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method: