β-Carotene in Serum/Plasma - HPLC
Fast and easy sample preparation
Low costs per test
High sample stability
Further analytes detectable: Cryptoxanthin, Lycopene, α-Carotene, cis-β-Carotene
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
All-trans-β-Carotene
Clinical relevance
β-carotene (provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. Like glutathione or vitamin C, it possesses antioxidative properties. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products.
β-carotene can only be processed by the body when absorbed together with fat. Any additional high-dosed preventive therapy should be used with caution to prevent a possible overdose. Additional use of high dose β-carotene over several years combined with nicotine and alcohol consumption may even increase the risk of lung or bowel cancer. For this reason, medications that contain β-carotene are labelled with a warning for smokers. Furthermore, drugs with high concentrations of β-carotene are no longer allowed to be prescribed to smokers either.
Product advantages
- Simple, fast and effective sample preparation
- High sample stability
- Low costs per test
This reagent kit is designed for easy and reliable determination of β-carotene in serum/plasma. The sample clean up procedure combines a fast precipitation and extraction step. The resulting extract is soluble in the mobile phase, so no evaporation is necessary. The chromatographic determination is run on an isocratic HPLC system with UV/VIS detection. A special RP-chromatography ensures a reliable separation of α-, cis-β-, and all-trans-β-carotene as well as some further carotenoids in less than 10 minutes. The analytes are quantified by the inclusion of an internal standard, which is a non-naturally occurring carotenoid-derivative, so that only a single detection wavelength is required. The high stability of the processed samples allows for easier performance of large sample batches.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | 5 ng/ml |
| Upper Limit of Quantification | up to 3000 ng/ml |
| Intraassay | CV ≤ 2.0 % |
| Interassay | CV ≤ 3.1 % |
| Recovery | 107 % |
| Specimen | Serum/Plasma |
| Sample Preparation |
|
| Run Time | approx. 10 min |
| Injection Volume | 50 µl |
| Flow Rate | 1.5-1.8 ml/min |
| Column Temperature | ambient (~25 °C) |
| Gradient | Isocratic |
| Wavelengths | 453 nm |
| Additional Info | Any isocratic HPLC system with pump, injector and UV detector is suitable. |
| Parameters | All-trans-β-Carotene |
All-trans-β-Carotene
Clinical relevance
β-carotene (provitamin A) is a precursor form of vitamin A, and is an essential nutrient for the human diet. Like glutathione or vitamin C, it possesses antioxidative properties. β-carotene is a herbal dye used as a colouring in the food industry and in vitamin products.
β-carotene can only be processed by the body when absorbed together with fat. Any additional high-dosed preventive therapy should be used with caution to prevent a possible overdose. Additional use of high dose β-carotene over several years combined with nicotine and alcohol consumption may even increase the risk of lung or bowel cancer. For this reason, medications that contain β-carotene are labelled with a warning for smokers. Furthermore, drugs with high concentrations of β-carotene are no longer allowed to be prescribed to smokers either.
Product advantages
- Simple, fast and effective sample preparation
- High sample stability
- Low costs per test
This reagent kit is designed for easy and reliable determination of β-carotene in serum/plasma. The sample clean up procedure combines a fast precipitation and extraction step. The resulting extract is soluble in the mobile phase, so no evaporation is necessary. The chromatographic determination is run on an isocratic HPLC system with UV/VIS detection. A special RP-chromatography ensures a reliable separation of α-, cis-β-, and all-trans-β-carotene as well as some further carotenoids in less than 10 minutes. The analytes are quantified by the inclusion of an internal standard, which is a non-naturally occurring carotenoid-derivative, so that only a single detection wavelength is required. The high stability of the processed samples allows for easier performance of large sample batches.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Lower Limit of Quantitation | 5 ng/ml |
| Upper Limit of Quantification | up to 3000 ng/ml |
| Intraassay | CV ≤ 2.0 % |
| Interassay | CV ≤ 3.1 % |
| Recovery | 107 % |
| Specimen | Serum/Plasma |
| Sample Preparation |
|
| Run Time | approx. 10 min |
| Injection Volume | 50 µl |
| Flow Rate | 1.5-1.8 ml/min |
| Column Temperature | ambient (~25 °C) |
| Gradient | Isocratic |
| Wavelengths | 453 nm |
| Additional Info | Any isocratic HPLC system with pump, injector and UV detector is suitable. |
| Parameters | All-trans-β-Carotene |