Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
Each analyte safeguarded by a specific internal standard
No post-column derivatisation required
One sample preparation and one run for both analytes
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
This Chromsystems kit allows for the HPLC determination of thiamine pyrophosphate and pyridoxal-5’-phosphate, analogues of Vitamin B1 and Vitamin B6, in one run, with one sample preparation and without complex post-column derivatisation. Sample preparation is restricted to a single precipitation step and subsequent derivatisation in a reaction vial. The derivatised research sample is analysed using fluorescence detection with a wavelength switching, and the chromatographic separation is performed with 2 mobile phases in less than 9 minutes. The use of a tailor-made internal standard for each parameter ensures precise and accurate results.
Method of Analysis | HPLC |
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Number of Tests | 100 |
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
Specimen | Whole Blood and Whole Blood/Plasma |
Sample Preparation |
|
Run Time | 7 - 9 min |
Injection Volume | 25-50 µl |
Flow Rate | 1.5 - 2.3 ml/min |
Column Temperature | ambient (~ 25 °C) |
Gradient | Gradient profile: specific binary gradients for whole blood and plasma |
Wavelengths | Start EX 320 nm, EM 415 nm |
Additional Info | For the combined analysis of vitamins B1 and B6 in whole blood or plasma every common HPLC system with binary pump and programmable fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
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Mobile Phase AOrder no.: 52001/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
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Mobile Phase BOrder no.: 52022/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
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Internal StandardOrder no.: 52044/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
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Precipitation ReagentOrder no.: 52005/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
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Neutralisation ReagentOrder no.: 52006/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
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Derivatisation Reagent 1Order no.: 52007/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
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Derivatisation Reagent 2Order no.: 52008/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
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HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
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Precolumn Cartridge 4/10Order no.: 18052/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
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HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
This Chromsystems kit allows for the HPLC determination of thiamine pyrophosphate and pyridoxal-5’-phosphate, analogues of Vitamin B1 and Vitamin B6, in one run, with one sample preparation and without complex post-column derivatisation. Sample preparation is restricted to a single precipitation step and subsequent derivatisation in a reaction vial. The derivatised research sample is analysed using fluorescence detection with a wavelength switching, and the chromatographic separation is performed with 2 mobile phases in less than 9 minutes. The use of a tailor-made internal standard for each parameter ensures precise and accurate results.
Method of Analysis | HPLC |
---|---|
Number of Tests | 100 |
Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
Specimen | Whole Blood and Whole Blood/Plasma |
Sample Preparation |
|
Run Time | 7 - 9 min |
Injection Volume | 25-50 µl |
Flow Rate | 1.5 - 2.3 ml/min |
Column Temperature | ambient (~ 25 °C) |
Gradient | Gradient profile: specific binary gradients for whole blood and plasma |
Wavelengths | Start EX 320 nm, EM 415 nm |
Additional Info | For the combined analysis of vitamins B1 and B6 in whole blood or plasma every common HPLC system with binary pump and programmable fluorescence detector is suitable. |
Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
-
Mobile Phase AOrder no.: 52001/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Mobile Phase BOrder no.: 52022/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Internal StandardOrder no.: 52044/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Precipitation ReagentOrder no.: 52005/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Neutralisation ReagentOrder no.: 52006/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Derivatisation Reagent 1Order no.: 52007/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 2Order no.: 52008/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
Precolumn Cartridge 4/10Order no.: 18052/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC
-
HPLC Column Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/PlasmaOrder no.: 52100
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC