MassChrom® Vitamins B1 and B6 in Whole Blood, with 96 Deep Well Plate
Fast and easy sample preparation
Robust method with high selectivity
Short run time of 2.5 minutes
Isotopically-labelled internal standard for each analyte
With 96 deep well plates
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
This assay enables combined analysis of the active forms of vitamin B1 (thiamine pyrophosphate, TPP) and vitamin B6 (pyridoxal 5‘-phosphate, PLP) in whole blood by LC-MS/MS. Sample preparation consists of a simple protein precipitation step, with no need for derivatisation. This makes for a much faster sample preparation. Analytes and potential interferences are effectively and swiftly separated within 2.5 minutes. The chromatographic separation of the MassChrom® assay is fast but sufficiently resolving for a clear separation of the two analytes. Isotope- labelled internal standards for both parameters as well as 6PLUS1® calibrators and MassCheck® controls ensure accurate and precise results.
The assay is available in 2 configurations either with reaction vials or with 96 deep well plates.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood |
| Sample Preparation |
|
| Run Time | 2.5 min |
| Injection Volume | 20 μl |
| Flow Rate | 0.5 ml/min |
| Column Temperature | 30 °C |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
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Internal Standard Set Vitamins B1 and B6Order no.: 87046/RUOMassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
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Pierceable adhesive seals for 96 Well PlatesOrder no.: 87059/RUO
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
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Analytical Column Vitamins B1 and B6 in Whole Blood - LC-MS/MSOrder no.: 87100MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
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6PLUS1® Multilevel Whole Blood Calibrator Set Vitamins B1 and B6Order no.: 87039MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS -
MassCheck® Vitamins B1 and B6 Whole Blood ControlsOrder no.: 0273/0274/0275MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS -
Tuning Mix Vitamins B1 and B6Order no.: 87015/RUO
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
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6PLUS1® Multilevel Whole Blood Calibrator Set Vitamins B1 and B6Order no.: 87039MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS
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MassCheck® Vitamins B1 and B6 Whole Blood ControlsOrder no.: 0273/0274/0275MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS
-
Analytical Column Vitamins B1 and B6 in Whole Blood - LC-MS/MSOrder no.: 87100
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
This assay enables combined analysis of the active forms of vitamin B1 (thiamine pyrophosphate, TPP) and vitamin B6 (pyridoxal 5‘-phosphate, PLP) in whole blood by LC-MS/MS. Sample preparation consists of a simple protein precipitation step, with no need for derivatisation. This makes for a much faster sample preparation. Analytes and potential interferences are effectively and swiftly separated within 2.5 minutes. The chromatographic separation of the MassChrom® assay is fast but sufficiently resolving for a clear separation of the two analytes. Isotope- labelled internal standards for both parameters as well as 6PLUS1® calibrators and MassCheck® controls ensure accurate and precise results.
The assay is available in 2 configurations either with reaction vials or with 96 deep well plates.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 480 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood |
| Sample Preparation |
|
| Run Time | 2.5 min |
| Injection Volume | 20 μl |
| Flow Rate | 0.5 ml/min |
| Column Temperature | 30 °C |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
-
Internal Standard Set Vitamins B1 and B6Order no.: 87046/RUO
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
-
-
-
-
-
Pierceable adhesive seals for 96 Well PlatesOrder no.: 87059/RUO
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
-
Analytical Column Vitamins B1 and B6 in Whole Blood - LC-MS/MSOrder no.: 87100
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
-
6PLUS1® Multilevel Whole Blood Calibrator Set Vitamins B1 and B6Order no.: 87039MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS
-
MassCheck® Vitamins B1 and B6 Whole Blood ControlsOrder no.: 0273/0274/0275MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS
-
Tuning Mix Vitamins B1 and B6Order no.: 87015/RUO
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS
-
-
-
6PLUS1® Multilevel Whole Blood Calibrator Set Vitamins B1 and B6Order no.: 87039MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS
-
MassCheck® Vitamins B1 and B6 Whole Blood ControlsOrder no.: 0273/0274/0275MassChrom® Vitamins B1 and B6 in Whole Blood – LC-MS/MS
-
Analytical Column Vitamins B1 and B6 in Whole Blood - LC-MS/MSOrder no.: 87100
MassChrom® Vitamins B1 and B6 in Whole Blood - LC-MS/MS