Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - UHPLC
UHPLC separation in less than 3 min
Each analyte is safeguarded by a specific internal standard
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
The Chromsystems UHPLC kit for the combined analysis of vitamin B1 in whole blood and vitamin B6 in whole blood or plasma research samples combines the features of the proven HPLC method with the distinct advantages of UHPLC technology. The efficient sample preparation with Pre-mixed Tubes involves only one precipitation step and subsequent derivatisation. Analyte separation takes approximately 3 minutes. Both analytes are safeguarded by specifically developed internal standards. Quality controls are also available separately.
Kit content
Mobile Phase A, 2 x 1000 ml
Mobile Phase B, 1000 ml
Whole Blood Calibration Standard (lyoph.), 5 x 5 x 1 ml
Internal Standard, 10 x 10 ml
Precipitation Reagent, 10 x 30 ml
Pre-mixed Neutralisation Tubes, 10 x 100 pcs.
Derivatisation Reagent 1 (lyoph.), 10 x 2 x 0.3 ml
Derivatisation Reagent 2 , 10 x 15 ml
Reaction Vials, amber coloured (light protection), 10 x 100 pcs.
UHPLC Column, 1 pc.
| Method of Analysis | UHPLC |
|---|---|
| Number of Tests | 1000 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood and Whole Blood/Plasma |
| Sample Preparation |
|
| Run Time | 3 min |
| Injection Volume | 2.5–10 μl |
| Flow Rate | 0.7 ml/min |
| Column Temperature | ambient (~ 25°C) |
| Wavelengths | Start: EX 320 nm, EM 415 nm |
| Additional Info | For the combined UHPLC analysis of vitamins B1 and B6 in whole blood and plasma, any major UHPLC system with binary pump and programmable fluorescence detector is suitable. |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
| Pressure | < 600 bar |
-
Internal StandardOrder no.: 52044/RUOVitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Precipitation ReagentOrder no.: 52005/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Pre-mixed Neutralisation TubesOrder no.: 52906/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 1Order no.: 52007/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 2Order no.: 52008/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
Vitamin B1 (TPP, thiamine pyrophosphate)
Vitamin B6 (PLP, pyridoxal 5’-phosphate)
The Chromsystems UHPLC kit for the combined analysis of vitamin B1 in whole blood and vitamin B6 in whole blood or plasma research samples combines the features of the proven HPLC method with the distinct advantages of UHPLC technology. The efficient sample preparation with Pre-mixed Tubes involves only one precipitation step and subsequent derivatisation. Analyte separation takes approximately 3 minutes. Both analytes are safeguarded by specifically developed internal standards. Quality controls are also available separately.
Kit content
Mobile Phase A, 2 x 1000 ml
Mobile Phase B, 1000 ml
Whole Blood Calibration Standard (lyoph.), 5 x 5 x 1 ml
Internal Standard, 10 x 10 ml
Precipitation Reagent, 10 x 30 ml
Pre-mixed Neutralisation Tubes, 10 x 100 pcs.
Derivatisation Reagent 1 (lyoph.), 10 x 2 x 0.3 ml
Derivatisation Reagent 2 , 10 x 15 ml
Reaction Vials, amber coloured (light protection), 10 x 100 pcs.
UHPLC Column, 1 pc.
| Method of Analysis | UHPLC |
|---|---|
| Number of Tests | 1000 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood and Whole Blood/Plasma |
| Sample Preparation |
|
| Run Time | 3 min |
| Injection Volume | 2.5–10 μl |
| Flow Rate | 0.7 ml/min |
| Column Temperature | ambient (~ 25°C) |
| Wavelengths | Start: EX 320 nm, EM 415 nm |
| Additional Info | For the combined UHPLC analysis of vitamins B1 and B6 in whole blood and plasma, any major UHPLC system with binary pump and programmable fluorescence detector is suitable. |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate) |
| Pressure | < 600 bar |
-
Internal StandardOrder no.: 52044/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Precipitation ReagentOrder no.: 52005/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Pre-mixed Neutralisation TubesOrder no.: 52906/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 1Order no.: 52007/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC
-
Derivatisation Reagent 2Order no.: 52008/RUO
Vitamin B1 in Whole Blood and Vitamin B6 in Whole Blood/Plasma - HPLC/UHPLC