MassChrom® Vitamins B1 and B6 in Whole Blood, with Reaction Vials

Order No.: 87000/RUO, for 480 tests
Parameters:
Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate)

Fast and easy sample preparation
Robust method with high selectivity
Short run time of 2.5 minutes
Isotopically-labelled internal standard for each analyte
With reaction vials

Vitamin B1 (TPP, thiamine pyrophosphate)

Vitamin B6 (PLP, pyridoxal 5’-phosphate)

This assay enables combined analysis of the active forms of vitamin B1 (thiamine pyrophosphate, TPP) and vitamin B6 (pyridoxal 5‘-phosphate, PLP) in whole blood by LC-MS/MS. Sample preparation consists of a simple protein precipitation step, with no need for derivatisation. This makes for a much faster sample preparation. Analytes and potential interferences are effectively and swiftly separated within 2.5 minutes. The chromatographic separation of the MassChrom® assay is fast but sufficiently resolving for a clear separation of the two analytes. Isotope- labelled internal standards for both parameters as well as 6PLUS1® calibrators and MassCheck® controls ensure accurate and precise results.

The assay is available in 2 configurations either with reaction vials or with 96 deep well plates.

More Information
Method of Analysis LC-MS/MS
Number of Tests 480
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual.
Specimen Whole Blood
Sample Preparation
  • Pipette 40 μl research sample/calibrator/MassCheck® control into reaction vial.
  • Add 80 μl Internal Standard Mix, mix briefly (vortex).
  • Add 350 μl Precipitation Reagent, mix for 1 min (vortex) and centrifuge for 2 min.
  • Transfer supernatant into autosampler vial.
  • Inject 20 μl into the LC-MS/MS system.
Run Time 2.5 min
Injection Volume 20 μl
Flow Rate 0.5 ml/min
Column Temperature 30 °C
Ionisation ESI positive
MS/MS Mode MRM
Parameters Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:

Vitamin B1 (TPP, thiamine pyrophosphate)

Vitamin B6 (PLP, pyridoxal 5’-phosphate)

This assay enables combined analysis of the active forms of vitamin B1 (thiamine pyrophosphate, TPP) and vitamin B6 (pyridoxal 5‘-phosphate, PLP) in whole blood by LC-MS/MS. Sample preparation consists of a simple protein precipitation step, with no need for derivatisation. This makes for a much faster sample preparation. Analytes and potential interferences are effectively and swiftly separated within 2.5 minutes. The chromatographic separation of the MassChrom® assay is fast but sufficiently resolving for a clear separation of the two analytes. Isotope- labelled internal standards for both parameters as well as 6PLUS1® calibrators and MassCheck® controls ensure accurate and precise results.

The assay is available in 2 configurations either with reaction vials or with 96 deep well plates.

More Information
Method of Analysis LC-MS/MS
Number of Tests 480
Please note The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual.
Specimen Whole Blood
Sample Preparation
  • Pipette 40 μl research sample/calibrator/MassCheck® control into reaction vial.
  • Add 80 μl Internal Standard Mix, mix briefly (vortex).
  • Add 350 μl Precipitation Reagent, mix for 1 min (vortex) and centrifuge for 2 min.
  • Transfer supernatant into autosampler vial.
  • Inject 20 μl into the LC-MS/MS system.
Run Time 2.5 min
Injection Volume 20 μl
Flow Rate 0.5 ml/min
Column Temperature 30 °C
Ionisation ESI positive
MS/MS Mode MRM
Parameters Vitamin B1 (TPP, thiamine pyrophosphate), Vitamin B6 (PLP, pyridoxal 5’-phosphate)
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method: