Vitamin B1 in Whole Blood - HPLC
No post-column derivatisation
Easy handling
Highly stable calibration standards and controls
Vitamin B1 (TPP, thiamine pyrophosphate)
The present method allows the rapid and simple determination of thiamine diphosphate, an analogue form of vitamin B1, in an isocratic HPLC system using fluorescent detection. The usual post-column derivatisation which requires extensive equipment is replaced here by a simple pre-column derivatisation. Sample preparation is restricted to a single precipitation step after addition of the extraction buffer and subsequent derivatisation in a reaction vial. A selected HPLC column and a mobile phase optimised for this specific separation permit high performance in HPLC quantification.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood |
| Sample Preparation |
|
| Run Time | approx. 5 min |
| Injection Volume | 50 µl |
| Flow Rate | 1 ml/min |
| Column Temperature | ambient (~25 °C) |
| Wavelengths | EX 367 nm EM 435 nm |
| Additional Info | For the analysis of vitamin B1 in whole blood every isocratic system with fluorescence detector is suitable. |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate) |
Vitamin B1 (TPP, thiamine pyrophosphate)
The present method allows the rapid and simple determination of thiamine diphosphate, an analogue form of vitamin B1, in an isocratic HPLC system using fluorescent detection. The usual post-column derivatisation which requires extensive equipment is replaced here by a simple pre-column derivatisation. Sample preparation is restricted to a single precipitation step after addition of the extraction buffer and subsequent derivatisation in a reaction vial. A selected HPLC column and a mobile phase optimised for this specific separation permit high performance in HPLC quantification.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Specimen | Whole Blood |
| Sample Preparation |
|
| Run Time | approx. 5 min |
| Injection Volume | 50 µl |
| Flow Rate | 1 ml/min |
| Column Temperature | ambient (~25 °C) |
| Wavelengths | EX 367 nm EM 435 nm |
| Additional Info | For the analysis of vitamin B1 in whole blood every isocratic system with fluorescence detector is suitable. |
| Parameters | Vitamin B1 (TPP, thiamine pyrophosphate) |