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Parameter Set Anti-HIV Drugs - LC-MS/MS

Order No.: 92924
Parameters:
Amprenavir, Atazanavir, Darunavir, Efavirenz, Elvitegravir, Etravirine, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Raltegravir, Ritonavir, Saquinavir

Encompasses 17 analytes
6PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

main product photo

Amprenavir

Atazanavir

Darunavir

Delavirdine

Efavirenz

Elvitegravir

Etravirine

Indinavir

Lopinavir

Maraviroc

Nelfinavir

Nelfinavir-M8

Nevirapine

Raltegravir

Rilpivirine

Ritonavir

Saquinavir

Clinical relevance

According to estimates from UNAIDS there are about 36.9 million people worldwide living with HIV, of which approximately 3 million are children under the age of 15 years. Even if the number of globally registered new infections is decreasing, it was still at approx. 2 million in 2014. The currently available drugs, which are usually prescribed as part of HAART (highly active antiretroviral therapy), interfere with or prevent the development of the virus at different stages of reproduction or cell infection.

Regular monitoring of drug concentration is very important, especially in HIV therapy. Sufficiently high levels of the administered antiretroviral drugs in plasma are a key factor in the success of the treatment. Individual plasma levels can fluctuate significantly and low levels can affect the success of the treatment. On the other hand high concentrations can cause major side effects, such as disorders of the central nervous system, liver toxicity, gastrointestinal disorders or renal toxicity. Therefore the measurement of drug concentrations in serum or plasma is essential for optimising the therapy.

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 1.0 – 65 µg/l
Upper Limit of Quantification up to 168 mg/l
Intraassay CV= 1.0 – 6.7 %
Interassay CV= 1.5 – 8.1 %
Recovery 88 – 111 %
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent (= mixture A).
  • Pipette 50 µl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system.
Run Time 3.0 – 3.5 min
Injection Volume 0.2 – 50 µl
Gradient

Group 1
0.00–0.20 min, 0 % Mobile Phase 2
0.21–2.00 min, 70 % Mobile Phase 2
2.01–2.90 min, 100 % Mobile Phase 2
2.91–3.50 min, 0 % Mobile Phase 2
Group 2
0.00–0.20 min, 0 % Mobile Phase 2
0.21–0.60 min, 50 % Mobile Phase 2
0.61–2.40 min, 90 % Mobile Phase 2
2.41–3.00 min, 0 % Mobile Phase 2
Group 3
0.00–0.20 min, 0 % Mobile Phase 2
0.21–2.30 min, 90 % Mobile Phase 2
2.31–3.10 min, 0 % Mobile Phase 2
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Amprenavir, Atazanavir, Darunavir, Efavirenz, Elvitegravir, Etravirine, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Raltegravir, Ritonavir, Saquinavir
The following components are included in the kit:
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® TDM Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  2. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  3. 92844 internal standard mix anti-hiv drugs
    Internal Standard Mix Anti-HIV Drugs
    Order no.: 92844
    Component of the Parameter Set Anti-HIV Drugs, available separately
    Details
The following products are not included in the kit but are required for the application of the method:
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® TDM Series A - LC-MS/MS

    Details
  2. 92042 LCMS TDM Series A tuning mix
    Tuning Mix Anti-HIV Drugs
    Order no.: 92042
    Tuning Mix for the Parameter Set Anti-HIV Drugs - LC-MS/MS
    Details
  3. 92111 LCMS TDM Series A Basic Kit
    Basic Kit A for 200 Tests - LC-MS/MS
    Order no.: 92111/200

    Part of the MassTox® TDM Series A
    Modular system for therapeutic drug monitoring
    Provides all components required for sample prep and all mobile phases

    Validated according to IVDR

    Details
  4. 92111 LCMS TDM Series A Basic Kit
    Basic Kit A for 1000 Tests - LC-MS/MS
    Order no.: 92111/1000

    Part of the MassTox® TDM Series A
    Modular system for therapeutic drug monitoring
    Provides all components required for sample prep and all mobile phases

    Validated according to IVDR

    Details
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® TDM Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  1. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. Reaction Vials, transparent, 1.5 ml
    Reaction Vials, transparent, 1.5 ml
    Order no.: 33006
    Details
  4. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  5. Stainless Steel Prefilter - 0.5 µm
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details
As a customer please login or register to gain full access.

Amprenavir

Atazanavir

Darunavir

Delavirdine

Efavirenz

Elvitegravir

Etravirine

Indinavir

Lopinavir

Maraviroc

Nelfinavir

Nelfinavir-M8

Nevirapine

Raltegravir

Rilpivirine

Ritonavir

Saquinavir

Clinical relevance

According to estimates from UNAIDS there are about 36.9 million people worldwide living with HIV, of which approximately 3 million are children under the age of 15 years. Even if the number of globally registered new infections is decreasing, it was still at approx. 2 million in 2014. The currently available drugs, which are usually prescribed as part of HAART (highly active antiretroviral therapy), interfere with or prevent the development of the virus at different stages of reproduction or cell infection.

Regular monitoring of drug concentration is very important, especially in HIV therapy. Sufficiently high levels of the administered antiretroviral drugs in plasma are a key factor in the success of the treatment. Individual plasma levels can fluctuate significantly and low levels can affect the success of the treatment. On the other hand high concentrations can cause major side effects, such as disorders of the central nervous system, liver toxicity, gastrointestinal disorders or renal toxicity. Therefore the measurement of drug concentrations in serum or plasma is essential for optimising the therapy.

 

MassTox® TDM Series A

The MassTox® TDM Series A is a modular system that enables the determination of 200 analytes without changing column or mobile phases, thereby minimising the workload in the laboratory.
It consists of 3 parts:
• MassTox® TDM Basic Kit A
• Specific MassTox® TDM Parameter Set (13 different parameter sets available)
• Analytical column MassTox® TDM MasterColumn® A

More information about MassTox® TDM Series A

More Information
Method of Analysis LC-MS/MS
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation 1.0 – 65 µg/l
Upper Limit of Quantification up to 168 mg/l
Intraassay CV= 1.0 – 6.7 %
Interassay CV= 1.5 – 8.1 %
Recovery 88 – 111 %
Specimen Serum/Plasma
Sample Preparation
  • Reconstitute the Internal Standard Mix
  • Add 800 µl Internal Standard Mix to 12 ml Precipitation Reagent (= mixture A).
  • Pipette 50 µl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial.
  • Add 25 µl Extraction Buffer, mix briefly (vortex) and incubate 2 min.
  • Add 250 µl of mixture A, mix 30 s (vortex) and centrifuge 5 min.
  • Dilute supernatant with Dilution Buffer (depending on instrument sensitivity) and inject into LC-MS/MS system.
Run Time 3.0 – 3.5 min
Injection Volume 0.2 – 50 µl
Gradient

Group 1
0.00–0.20 min, 0 % Mobile Phase 2
0.21–2.00 min, 70 % Mobile Phase 2
2.01–2.90 min, 100 % Mobile Phase 2
2.91–3.50 min, 0 % Mobile Phase 2
Group 2
0.00–0.20 min, 0 % Mobile Phase 2
0.21–0.60 min, 50 % Mobile Phase 2
0.61–2.40 min, 90 % Mobile Phase 2
2.41–3.00 min, 0 % Mobile Phase 2
Group 3
0.00–0.20 min, 0 % Mobile Phase 2
0.21–2.30 min, 90 % Mobile Phase 2
2.31–3.10 min, 0 % Mobile Phase 2
Ionisation ESI positive
MS/MS Mode MRM
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Parameters Amprenavir, Atazanavir, Darunavir, Efavirenz, Elvitegravir, Etravirine, Indinavir, Lopinavir, Maraviroc, Nelfinavir, Nelfinavir-M8, Raltegravir, Ritonavir, Saquinavir
The following components are included in the kit:
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® TDM Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  2. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  3. 92844 internal standard mix anti-hiv drugs
    Internal Standard Mix Anti-HIV Drugs
    Order no.: 92844
    Component of the Parameter Set Anti-HIV Drugs, available separately
    Details
The following products are not included in the kit but are required for the application of the method:
  1. 92110 TDM Series A Master Column
    MassTox® TDM MasterColumn® A
    Order no.: 92110

    Analytical column for MassTox® TDM Series A - LC-MS/MS

    Details
  2. 92042 LCMS TDM Series A tuning mix
    Tuning Mix Anti-HIV Drugs
    Order no.: 92042
    Tuning Mix for the Parameter Set Anti-HIV Drugs - LC-MS/MS
    Details
  3. 92111 LCMS TDM Series A Basic Kit
    Basic Kit A for 200 Tests - LC-MS/MS
    Order no.: 92111/200

    Part of the MassTox® TDM Series A
    Modular system for therapeutic drug monitoring
    Provides all components required for sample prep and all mobile phases

    Validated according to IVDR

    Details
  4. 92111 LCMS TDM Series A Basic Kit
    Basic Kit A for 1000 Tests - LC-MS/MS
    Order no.: 92111/1000

    Part of the MassTox® TDM Series A
    Modular system for therapeutic drug monitoring
    Provides all components required for sample prep and all mobile phases

    Validated according to IVDR

    Details
  1. 92053 LCMS TDM Series A 6PLUS1 calibrator anti-HIV drugs
    6PLUS1® Multilevel Plasma Calibrator Set Anti-HIV Drugs
    Order no.: 92053
    MassTox® TDM Series A Anti-HIV Drugs in Serum/Plasma – LC-MS/MS
    Details
  1. 0261 0262 0263 LCMS TDM Series A controls anti-HIV drugs
    MassCheck® Anti-HIV Drugs Plasma Controls
    Order no.: 0261/0262/0263
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    PEEK-encased Prefilter, 2 µm
    Order no.: 15011
    Details
  3. Reaction Vials, transparent, 1.5 ml
    Reaction Vials, transparent, 1.5 ml
    Order no.: 33006
    Details
  4. 15070 15071 Prefilter and Housing
    Stainless Steel Prefilter Housing
    Order no.: 15070
    Details
  5. Stainless Steel Prefilter - 0.5 µm
    Stainless Steel Prefilter - 0.5 µm
    Order no.: 15071
    Details
As a customer please login or register to gain full access.