Metanephrines in Urine, Combined Analysis - HPLC
Short analysis time < 10 min
Only 1 HPLC column and 1 Mobile Phase for 2020-C and 6000-C
Assay validated according to IVDR (=> Declaration of Conformity)
Chromatogram for 2020-C (Metanephrines in Urine, Combined Analysis)
Chromatogram for 6000-C (Catecholamines in Urine, Combined Analysis)
using the same HPLC column and mobile phase as kit 2020-C
With kit 2020-C
3-Methoxytyramine
Metanephrine
Normetanephrine
With kit 6000-C, using the same HPLC column and mobile phase as kit 2020-C
Adrenaline (Epinephrine)
Dopamine
Noradrenaline
Clinical relevance
This assay allows the simultaneous quantitative determination of total (sum of free and conjugated metabolites) metanephrine, normetanephrine and 3-methoxytyramine in human urine samples by HPLC.
It is intended to be used for patients in whom the urinary levels of total normetanephrine, total metanephrine and total 3-methoxytyramine are of clinical importance, primarily as an aid to diagnosis and monitoring of suspected pheochromocytoma and paraganglioma
Furthermore, it is intended for patients in whom the urinary levels of total normetanephrine and total 3-methoxytyramine are of clinical importance, as an aid to diagnosis and monitoring of suspected neuroblastoma.
Product advantages
• Short run time of 10 min (Internal Standard 2024) or 13 min (Internal Standard High Resolution 2044/HR)
• Only 1 HPLC column and 1 mobile phase for 2020-C and 6000-C => No changes to setup are needed due to identical column and mobile phase.
Assay characteristics
Sample preparation is carried out manually, and analytic separation is done via high performance liquid chromatography with electrochemical detection (HPLC-ECD). The use of a specific internal standard ensures high precision and reliability in quantitation of the analytes.
Combined analysis
The same HPLC column and mobile phase can be used for both catecholamine (2020-C) and metanephrine (6000-C) analysis.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Normetanephrine: 6.8 µg/L – 3000 µg/L |
| Specimen | 24-hour urine. Alternatively spot urine may also be used. In this case, results must be related to creatinine. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Acidic hydrolysis Neutralisation Extraction Continued sample preparation prior to injection |
| Run Time | 10 min (Internal Standard 2024) --- 13 min (Internal Standard High Resolution 2044/HR) |
| Injection Volume | 10 µL |
| Flow Rate | 1.3 mL/min |
| Column Temperature | 30 °C |
| Potential | approx. +780 mV to +840 mV |
| Additional Info | For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used. |
| Parameters | 3-Methoxytyramine, Metanephrine, Normetanephrine |
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Mobile PhaseOrder no.: 2031/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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Stabilisation ReagentOrder no.: 2029/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
Chromatogram for 2020-C (Metanephrines in Urine, Combined Analysis)
Chromatogram for 6000-C (Catecholamines in Urine, Combined Analysis)
using the same HPLC column and mobile phase as kit 2020-C
With kit 2020-C
3-Methoxytyramine
Metanephrine
Normetanephrine
With kit 6000-C, using the same HPLC column and mobile phase as kit 2020-C
Adrenaline (Epinephrine)
Dopamine
Noradrenaline
Clinical relevance
This assay allows the simultaneous quantitative determination of total (sum of free and conjugated metabolites) metanephrine, normetanephrine and 3-methoxytyramine in human urine samples by HPLC.
It is intended to be used for patients in whom the urinary levels of total normetanephrine, total metanephrine and total 3-methoxytyramine are of clinical importance, primarily as an aid to diagnosis and monitoring of suspected pheochromocytoma and paraganglioma
Furthermore, it is intended for patients in whom the urinary levels of total normetanephrine and total 3-methoxytyramine are of clinical importance, as an aid to diagnosis and monitoring of suspected neuroblastoma.
Product advantages
• Short run time of 10 min (Internal Standard 2024) or 13 min (Internal Standard High Resolution 2044/HR)
• Only 1 HPLC column and 1 mobile phase for 2020-C and 6000-C => No changes to setup are needed due to identical column and mobile phase.
Assay characteristics
Sample preparation is carried out manually, and analytic separation is done via high performance liquid chromatography with electrochemical detection (HPLC-ECD). The use of a specific internal standard ensures high precision and reliability in quantitation of the analytes.
Combined analysis
The same HPLC column and mobile phase can be used for both catecholamine (2020-C) and metanephrine (6000-C) analysis.
Detailed performance evaluation data can be found in Appendices II and III of the instruction manual.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data can be found in Appendices II and III of the instruction manual. |
| Lower and Upper Limit of Quantitation | Normetanephrine: 6.8 µg/L – 3000 µg/L |
| Specimen | 24-hour urine. Alternatively spot urine may also be used. In this case, results must be related to creatinine. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual. Acidic hydrolysis Neutralisation Extraction Continued sample preparation prior to injection |
| Run Time | 10 min (Internal Standard 2024) --- 13 min (Internal Standard High Resolution 2044/HR) |
| Injection Volume | 10 µL |
| Flow Rate | 1.3 mL/min |
| Column Temperature | 30 °C |
| Potential | approx. +780 mV to +840 mV |
| Additional Info | For the combined HPLC analysis of catecholamines and metanephrines any isocratic HPLC system with electrochemical detector can be used. |
| Parameters | 3-Methoxytyramine, Metanephrine, Normetanephrine |
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Mobile PhaseOrder no.: 2031/COMBI
Combined analysis of catecholamines/metanephrines in urine - HPLC
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Stabilisation ReagentOrder no.: 2029/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC
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HPLC Column Catecholamines/Metanephrines in UrineOrder no.: 2130/COMBICombined analysis of catecholamines/metanephrines in urine - HPLC