MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma for automated sample preparation - LC-MS/MS
Suitable for different liquid handling devices
Sample tracking, LIMS connectivity and full walkaway operation for improved workflows
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746


25-OH-Vitamin D2
25-OH-Vitamin D3
Clinical relevance
Vitamin D (cholecalciferol) is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.
25-OH-vitamin D3 is the primary metabolite in the human body and the direct precursor of the physiologically active form 1.25-(OH)2-vitamin D3. First-line treatment for vitamin D deficiency is nutritional supplements with vitamin D3 or vitamin D2. Vitamin D2 is easier to synthesise and is widely used as a supplement outside of the EU, and especially in the United States. Therefore, its determination in serum/plasma is also relevant.
Product advantages
- Automated sample preparation in 96 Well Filter Plates
- 3PLUS1® or 6PLUS1® Multilevel Calibrator Set and MassCheck® Controls
- Isotopically labelled internal standard
This Chromsystems kit allows the determination of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma with LC-MS/MS. 96 samples can be reliably processed in 30 minutes using automated sample preparation. The comparison of manual and automated method shows a strong correlation. The 3PLUS1® and 6PLUS1® multilevel calibrators are optionally available and guarantee a high level of precision of the results.
A variant for manual sample preparation in 96 well filter plates (order no. 62000) is also available.
Content for 1000 tests
Mobile Phase A, 5 x 1000 ml
Mobile Phase B, 5 x 1000 ml
Precipitation Reagent, 5 x 5 ml
Internal Standard, 5 x 40 ml
Rinsing Solution, 2 x 1000 ml
96 Well Filter Plates, 4 x 3 pcs.
Collection Plates, 4 x 3 pcs.
Pierceable Adhesive Seals for 96 Well Plates, 4 x 3 pcs.
3PLUS1® Multilevel Serum Calibrator Set (lyoph.), 4 x 1 ml
MassCheck® 25-OH-vitamin D3/D2 Serum Control, Level I (lyoph.), 5 x 1 ml
MassCheck® 25-OH-vitamin D3/D2 Serum Control, Level II (lyoph.), 5 x 1 ml
Analytical Column, 1 pc.
Trap Column, 2 pcs.
Method of Analysis | LC-MS/MS |
---|---|
Number of Tests | 1000 |
Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Different systems might show different performance data. |
Lower Limit of Quantitation | 25-OH-Vitamin D2: 2.2 µg/l |
Upper Limit of Quantification | up to 250 μg/l |
Intraassay | CV < 5 % |
Interassay | CV < 6 % |
Specimen | Serum/Plasma |
Sample Preparation | Samples can be prepared with full or partial automation on a liquid handling device. Manual sample preparation using 96 well filter plates is also possible. A multichannel pipette (8-channel or 12-channel) or a Multipette may be used. |
Run Time | 5 min |
Additional Info | Pump A delivers 100 % Mobile Phase A for loading the trap column. |
Ionisation | APCI positive |
MS/MS Mode | MRM |
Information | The trap column and the analytical column are linked to the mass spectrometer through a 2-position 6-port valve. |
Parameters | 25-OH-Vitamin D2, 25-OH-Vitamin D3 |
Flow | Pump A: 0.02–1.00 ml/min |
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Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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Tuning Mix 25-OH-Vitamin D3/D2Order no.: 62015
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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Tuning Mix 25-OH-Vitamin D3/D2Order no.: 62015
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
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Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS


25-OH-Vitamin D2
25-OH-Vitamin D3
Clinical relevance
Vitamin D (cholecalciferol) is a recognised clinical chemical determinant associated with osteoporosis and osteopenia (reduction in bone density). It also plays a role in the body's immune response, cardiovascular diseases and some types of cancer. Therefore, vitamin D is highly important from a number of diagnostic perspectives.
25-OH-vitamin D3 is the primary metabolite in the human body and the direct precursor of the physiologically active form 1.25-(OH)2-vitamin D3. First-line treatment for vitamin D deficiency is nutritional supplements with vitamin D3 or vitamin D2. Vitamin D2 is easier to synthesise and is widely used as a supplement outside of the EU, and especially in the United States. Therefore, its determination in serum/plasma is also relevant.
Product advantages
- Automated sample preparation in 96 Well Filter Plates
- 3PLUS1® or 6PLUS1® Multilevel Calibrator Set and MassCheck® Controls
- Isotopically labelled internal standard
This Chromsystems kit allows the determination of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma with LC-MS/MS. 96 samples can be reliably processed in 30 minutes using automated sample preparation. The comparison of manual and automated method shows a strong correlation. The 3PLUS1® and 6PLUS1® multilevel calibrators are optionally available and guarantee a high level of precision of the results.
A variant for manual sample preparation in 96 well filter plates (order no. 62000) is also available.
Content for 1000 tests
Mobile Phase A, 5 x 1000 ml
Mobile Phase B, 5 x 1000 ml
Precipitation Reagent, 5 x 5 ml
Internal Standard, 5 x 40 ml
Rinsing Solution, 2 x 1000 ml
96 Well Filter Plates, 4 x 3 pcs.
Collection Plates, 4 x 3 pcs.
Pierceable Adhesive Seals for 96 Well Plates, 4 x 3 pcs.
3PLUS1® Multilevel Serum Calibrator Set (lyoph.), 4 x 1 ml
MassCheck® 25-OH-vitamin D3/D2 Serum Control, Level I (lyoph.), 5 x 1 ml
MassCheck® 25-OH-vitamin D3/D2 Serum Control, Level II (lyoph.), 5 x 1 ml
Analytical Column, 1 pc.
Trap Column, 2 pcs.
Method of Analysis | LC-MS/MS |
---|---|
Number of Tests | 1000 |
Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Different systems might show different performance data. |
Lower Limit of Quantitation | 25-OH-Vitamin D2: 2.2 µg/l |
Upper Limit of Quantification | up to 250 μg/l |
Intraassay | CV < 5 % |
Interassay | CV < 6 % |
Specimen | Serum/Plasma |
Sample Preparation | Samples can be prepared with full or partial automation on a liquid handling device. Manual sample preparation using 96 well filter plates is also possible. A multichannel pipette (8-channel or 12-channel) or a Multipette may be used. |
Run Time | 5 min |
Additional Info | Pump A delivers 100 % Mobile Phase A for loading the trap column. |
Ionisation | APCI positive |
MS/MS Mode | MRM |
Information | The trap column and the analytical column are linked to the mass spectrometer through a 2-position 6-port valve. |
Parameters | 25-OH-Vitamin D2, 25-OH-Vitamin D3 |
Flow | Pump A: 0.02–1.00 ml/min |
-
Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
-
Tuning Mix 25-OH-Vitamin D3/D2Order no.: 62015
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
-
Tuning Mix 25-OH-Vitamin D3/D2Order no.: 62015
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS
-
Analytical Column 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MSOrder no.: 62100
MassChrom® 25-OH-Vitamin D3/D2 in Serum/Plasma - LC-MS/MS