Serotonin in Serum/Plasma/Whole Blood - HPLC
Easy sample preparation
Short run time
Long column life time
Serotonin (5-hydroxytryptamine) is a biogenic amine. In humans, serotonin is involved in a large number of physiological processes, e.g. it acts as a tissue hormone and neurotransmitter in the central nervous system, the enteric nervous system and the cardiovascular system. A large amount of serotonin is found in the chromaffin cells of the gastrointestinal tract where it is part of the gastrointestinal hormone system. When released into the capillary blood, serotonin is almost absorbed by the thrombocytes.
A number of diseases are associated with pathological changes in the serotonin metabolism. For the routine, the diagnosis of carcinoid tumours together with the analysis of 5-hydroxyindoleacetic acid (5-HIAA) is very important.
- Simple and efficient sample preparation
- Fast results due to short analysis times
- Long column life times
This kit allows for reliable HPLC determination of serotonin in serum/plasma and whole blood as part of routine diagnostic laboratory testing. Easy and fast sample preparation leads to exact and reproducible results. The chromatographic determination requires an isocratic HPLC system with electrochemical detection. Calibrator and quality controls are available separately – they are produced from pure human matrices to prevent matrix-associated measurement variabilities.
Alternatively, Chromsystems offers a kit for determining serotonin in urine (# 4000).
Mobile Phase, 1000 ml
Serotonin Calibration Standard, 10 ml
Internal Standard, 10 ml
Precipitation Reagent, 10 ml
Reaction vials, 100 pcs.
|Method of Analysis||HPLC|
|Number of Tests||100|
|Limit of quantification||1 µg/l|
|Linearity||up to 1000 µg/l|
|Recovery||> 95 %|
CV < 2 %
CV < 1.5 %
|Analysis Time||< 8 min|
Time of sample drawing
Care has to be taken to destroy all platelets completely. Recommendation: intensive clotting by the use of serum tubes with clot activator. Centrifuge at least 10 min at 1500 g [Reference: DG Klinische Chemie Mitteilungen 1998; 29(3)].
Plasma samples (platelet-poor plasma)
When measuring serotonin in plasma it is essential to obtain cell-free plasma without destruction of platelets. For this purpose the addition of an anticoagulant is necessary. Recommendation: Centrifuge minimum 15 min at 2000 to 3000 g [Reference: DG Klinische Chemie Mitteilungen 1998; 29(3)].
Centrifuge EDTA-whole blood at low speed (1500 rpm, 15 min). The obtained platelet-rich plasma is centrifuged again at high speed (4000 to 5000 rpm, 5 to 10 min), and the platelet concentrate can be collected from the bottom of the tube.
Whole blood specimens
|Sample Stability||At +2 to +8 °C up to 12 hours. Freeze the samples if they are not analysed within a period of 12 hours after blood collection.|
|Injection Volume||20 µl|
|Flow rate||1.0 to 1.2 ml/min|
|Column temperature||ambient (~25 °C)|
For the HPLC analysis of serotonin any isocratic HPLC system with electrochemical detector is suitable.
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Potential||approx. 400 to 500 mV|