Sample Prep Set VMA, HVA, 5-HIAA with Autosampler Vials
Simple and fast sample prep
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
5-Hydroxyindoleacetic Acid (5-HIAA)
Homovanillic Acid (HVA)
Vanillylmandelic Acid (VMA)
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With autosampler vials
Clinical Relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these metabolites are of clinical importance.
Product advantage
- Simple and fast sample prep
- Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using collection plates (80800/96 & 80800/480).
This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | 5-Hydroxyindoleacetic acid (5-HIAA): 2.30 mg/l – 150 mg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 3 min |
| Injection Volume | ≤ 2 µl or ≤ 10 µl (mass spectrometer dependent) |
| Column Temperature | +25 °C |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA) |
-
Chromatographic Platform Starter SetOrder no.: 80000MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS -
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
System Check Solution SetOrder no.: 80810MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Tuning Mix VMA, HVA, 5-HIAAOrder no.: 80815MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Analytical Column VMA, HVA, 5-HIAA Urine - LC-MS/MSOrder no.: 80200MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
In addition to a Sample Prep Set the products of the Chromatographic Platform are required.
5-Hydroxyindoleacetic Acid (5-HIAA)
Homovanillic Acid (HVA)
Vanillylmandelic Acid (VMA)
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
With autosampler vials
Clinical Relevance
This Sample Prep Set – in combination with the chromatographic platform (order no. 80000) – allows the quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) in patient urine samples by LC-MS/MS.
It is used as a test for patients in whom the urine levels of these metabolites are of clinical importance.
Product advantage
- Simple and fast sample prep
- Isotopically labelled internal standard for each analyte
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls available
Alternatively, there is also a method available using collection plates (80800/96 & 80800/480).
This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.
In addition to the Sample Prep Set, the products of the Chromatographic Platform are required.
These are the analytical column, mobile phases A and B and the rinsing solution.
| Method of Analysis | LC-MS/MS |
|---|---|
| Number of Tests | 96 |
| Please note | The information listed here, including the sample preparation, is not sufficient for using the product. Please read the information provided in the instruction manual, which includes detailed information on limitations associated with the use of the product in line with its intended purpose. Detailed performance evaluation data for this assay can be found in the appendices of the instruction manual. |
| Lower and Upper Limit of Quantitation | 5-Hydroxyindoleacetic acid (5-HIAA): 2.30 mg/l – 150 mg/l Different systems might show different performance data. |
| Specimen | 24-hour urine or spontaneous urine. Detailed information can be found in the instruction manual. |
| Sample Preparation | The information on the sample preparation presented here is not sufficient for use in the laboratory. For a detailed step by step description, please refer to the instruction manual.
|
| Run Time | 3 min |
| Injection Volume | ≤ 2 µl or ≤ 10 µl (mass spectrometer dependent) |
| Column Temperature | +25 °C |
| Ionisation | ESI positive |
| MS/MS Mode | MRM |
| Parameters | 5-Hydroxyindoleacetic Acid (5-HIAA), Homovanillic Acid (HVA), Vanillylmandelic Acid (VMA) |
-
Chromatographic Platform Starter SetOrder no.: 80000
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
Identical chromatographic platform for all Sample Prep Sets
Contains analytical column, mobile phases, rinsing solution -
6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
System Check Solution SetOrder no.: 80810
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Tuning Mix VMA, HVA, 5-HIAAOrder no.: 80815
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
Analytical Column VMA, HVA, 5-HIAA Urine - LC-MS/MSOrder no.: 80200
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS
-
6PLUS1® Multilevel Urine Calibrator Set VMA, HVA, 5-HIAAOrder no.: 80839MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
-
MassCheck® VMA, HVA, 5-HIAA Urine ControlsOrder no.: 0381/0382
MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS