Sample Prep Set Catecholamines, free Metanephrines, Serotonin with 96 SPE Well Plate

Order No.: 80600/96, for 96 tests
Parameters:
3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin

7 Analytes with 1 Sample Prep Set
Isotopically labelled internal standard for each analyte
6PLUS1® Multilevel Calibrator Set and MassCheck® controls
Fully automated workflow solution available

In addition to a Sample Prep Set the products of the Chromatographic Platform are required.

3-Methoxytyramine

Adrenaline (Epinephrine)

Noradrenaline

Dopamine

Metanephrine

Normetanephrine

Serotonin

MassChrom® Biogenic Amines/Metabolites in Urine – LC-MS/MS
With 96 SPE well plates for 96 analyses (80600/96) or 480 analyses (80600/480), suitable for manual and automated sample prep procedure

 

Clinical relevance

This Sample Prep Set allows the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine), dopamine, 3-methoxytyramine, metanephrine, normetanephrine and serotonin in patient urine samples by LC-MS/MS. One typical application for this kit is the analysis of patient samples with suspected catecholamine or serotonin secreting tumours.

 

Product advantages

  • 7 analytes in one single run
  • Fast and efficient sample prep
    Correct pH, required for a precise analysis, is verified quickly and conveniently by a colour indicator and can be adjusted with a specific reagent, if necessary
  • Isotopically labelled internal standard for each analyte
  • 6PLUS1® Multilevel Calibrator Set and MassCheck® controls ensure high precision of results
  • Fully automated workflow solution available


This Sample Prep Set encompasses all required components for an efficient and fast sample preparation.

A fully automated workflow solution is also available.

Alternatively, there is also a method available using Sample Clean Up Columns (80600/C).

More Information
Number of Tests 96
Limit of quantification 1 - 8 µg/l
Linearity 1600 - 5000 µg/l
Intraassay CV 1.7 - 6.6 %
Interassay CV 3.3 - 10.7 %
Analysis Time 4 min
Sample Preparation
  • Put 96 SPE Well Plate onto a Collection Plate.
  • Pipette 200 µl of the sample/calibrator/MassCheck® control into each well of the 96 SPE Well Plate.
  • Add 50 µl Internal Standard Mix and 600 µl Neutralisation Buffer.
  • Check for correct colour.
  • Centrifuge 5 min at 150 x g, discard effluent.
  • Add 0.9 ml Wash Buffer 1 and centrifuge 5 min at 150 x g, discard effluent.
  • Add 2 x 0.9 ml Wash Buffer 2 and centrifuge each time 1 min at 150 x g, discard effluent
  • Centrifuge 1 min at 2000 x g to dryness, discard effluent.
  • Place 96 SPE Well Plate onto a new Collection Plate.
  • Add 500 µl Elution Buffer to each well and centrifuge 2 min at 100 x g.
  • Add 500 µl Dilution Buffer to each well of the Collection Plate.
  • Seal Collection Plate with adhesive seal.
  • Shake for 2 min at 900 rpm and transfer to autosampler.
  • Inject up to 10 µl.
Sample Stability The prepared samples are stable for at least 5 days at +2°C to +8°C in amber coloured vials. For longer storage periods keep samples frozen below -18°C.
Specimen 24 h urine or spontaneous urine
Pre-analytic Treatment

24 h urine collected in a suitable container with 10 ml of 10–25 % HCl.
Spontaneous urine can be also analysed (see instruction manual).

 

Patient specimens are stable for at least 7 days at +2 to +8 °C in amber coloured vials.
For longer storage periods keep samples frozen below -18 °C.

Injection Volume ≤ 10 µl
Ionisation ESI positive
MS/MS-Mode MRM
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis LC-MS/MS
Parameter 3-Methoxytyramine, Adrenaline (Epinephrine), Dopamine, Metanephrine, Noradrenaline, Normetanephrine, Serotonin
  1. Internal Standard Mix
    Internal Standard Mix
    Order no.: 80604

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  2. Neutralisation Buffer
    Neutralisation Buffer
    Order no.: 80605

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  3. Wash Buffer 1
    Wash Buffer 1
    Order no.: 80606

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  4. Wash Buffer 2
    Wash Buffer 2
    Order no.: 80607

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  5. Elution Buffer
    Elution Buffer
    Order no.: 80608

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  6. Dilution Buffer
    Dilution Buffer
    Order no.: 80609

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  7. 80056 LCMS Biogenic amines collection plate
    Collection Plates
    Order no.: 80056

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  8. 96 SPE Well Plate
    96 SPE Well Plate
    Order no.: 80057

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  9. Pierceable adhesive seals for 96 Well Plates
    Pierceable adhesive seals for 96 Well Plates
    Order no.: 80059

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  1. 6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, Serotonin
    6PLUS1® Multilevel Urine Calibrator Set Catecholamines, free Metanephrines, Serotonin
    Order no.: 80639

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  1. MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Controls
    MassCheck® Catecholamines, free Metanephrines, Serotonin Urine Controls
    Order no.: 0372/0373

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  1. Tuning Mix MassChrom® Biogenic Amines/Metabolites
    Tuning Mix MassChrom® Biogenic Amines/Metabolites
    Order no.: 80615

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  2. System Check Solution
    System Check Solution
    Order no.: 80610

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
  3. Adjustment Reagent
    Adjustment Reagent
    Order no.: 80603

    MassChrom® Biogenic Amines/Metabolites in Urine - LC-MS/MS

    Details
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