Porphyrins in Urine - HPLC
Easy sample preparation
Specifically developed internal standard
Stable standards and controls
Coproporphyrin I
Coproporphyrin III
Heptacarboxyporphyrin
Hexacarboxyporphyrin
Pentacarboxyporphyrin
Uroporphyrin
Clinical relevance
Porphyrins are precursors of haem and usually only occur in urine in negligible amounts. However, disorders in haem biosynthesis lead to the syndrome of porphyria with increased formation and excretion of porphyrins. Porphyrias can be hereditary-related or acquired through exogenous influences such as alcohol consumption or lead poisoning. Different enzymes of the haem biosynthesis can be deficient, thus, several different porphyrias can be distinguished. Since these share some common symptoms, chromatographic measurement of the porphyrins is the only way to enable an exact diagnosis.
Product advantages
- Easy sample preparation
- Custom-made internal standard
- Stable standards and controls
This kit is designed for the fast and reliable determination of all porphyrins in urine that are relevant to differential diagnosis. Sample preparation requires only the addition of an internal standard and a precipitation reagent, followed by the centrifugation of the urine. The internal standard elutes in the middle of the chromatogram, making it easier to interpret the results of the measurement.
Kit content
Mobile Phase A, 1000 ml
Mobile Phase B, 1000 ml
Porphyrins Urine Calibration Standard (lyoph.), 5 x 2 ml
Internal Standard, 5 ml
Stabilisation Reagent, 5 ml
Priming Solution, 5 ml
Reaction vials, amber coloured (light protection), 100 pcs.
| Method of Analysis | HPLC |
|---|---|
| Number of Tests | 100 |
| Limit of quantification | 3 µg/l |
| Linearity | up to 5000 µg/l |
| Recovery | 100–106 % |
| Intraassay | CV ≤ 3.9 % |
| Interassay | CV ≤ 6 % |
| Analysis Time | 15 min + approx. 5 min reequilibration |
| Specimen | Urine |
| Pre-analytic Treatment | Keep patient specimens cool and light protected during transport. At a pH value of 6 to 7 storage life is up to 2 days at +2 to +8 °C. For longer storage (up to 1 month) keep samples below -18 °C. Stabilize acidic urines by sodium hydrogen carbonate (attention: foaming). Avoid repeated freeze/thaw cycles! |
| Sample Preparation |
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| Sample Stability | The prepared samples are stable at ambient temperature at least for 24 h, at +2 to +8 °C or below -18 °C up to 6 days. |
| Injection Volume | 25 µl |
| Flow rate | 1.2 ml/min |
| Column temperature | ambient (+18 to +30 °C) |
| Wavelength | EX 405 nm |
| Gradient | binary |
| Additional Info | Any binary HPLC gradient system with fluorescence detector is suitable. |
| Please note | The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals. |
| Parameter | Coproporphyrin I, Coproporphyrin III, Heptacarboxyporphyrin, Hexacarboxyporphyrin, Pentacarboxyporphyrin, Uroporphyrin |
