Parameter Set Benzodiazepines 2 - LC-MS/MS

Order No.: 92918
Alprazolam, Bromazepam, Clonazepam, Desalkylflurazepam, Flunitrazepam, Flurazepam, Lorazepam, Lormetazepam, Nitrazepam, Triazolam

Encompasses 10 analytes
3PLUS1® Multilevel Calibrator Set available
Part of the MassTox® TDM Series A

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation











Clinical relevance

Benzodiazepines (BZD) are a group of psychoactive drugs with similar structures that are classified as sedatives. By virtue of their high lipophilicity they penetrate the blood-brain barrier easily and, after being resorbed, quickly reach the central nervous system, where they enhance the impact of the inhibiting neurotransmitter GABA. Besides anxiolytic and sedative effects, BZD also have anti-aggressive, hypnotic, muscle relaxant and anticonvulsive properties. The predominance of the individual effects varies with the special structures of the benzodiazepines and the administered dose. BZD in blood are present mostly bound nonspecifically to plasma proteins. They are metabolised mainly by the liver. The resulting metabolites are mostly pharmacologically active themselves, and in some cases they represent the actual active substance, e.g. nordiazem being metabolised from diazepam or clorazepate. The plasma elimination times are highly variable: from a few hours (midazolam) to several days (clobazam). The metabolites frequently have longer half-lives than the parent substance, so an enrichment of therapeutically effective substances in the blood can occur.


Product advantages

With the Parameter Set Benzodiazepines 2 in serum/plasma, 10 different drugs can be measured quickly and efficiently using LC-MS/MS. By carefully optimising all of the kit components and the chromatographic separation, matrix effects (“ion suppression”) have been minimised and the robustness of the method has been enhanced. Sample preparation is based on protein precipitation. The use of stable isotopically labelled (deuterated) and co-eluting internal standards as well as 3PLUS1® multilevel calibrators ensures high precision and reproducible and reliable quantification of the analytes. The method is comprehensively validated.

The Parameter Set is a part of the Series A modular system, which enables the analysis of all parameters without switching column or changing the mobile phases, thereby minimising required workload in the laboratory. The Basic Kit A contains all components required for sample preparation and all necessary mobile phases. The MasterColumn® A is the analytical column used for the determination of all Series A analytes. Our portfolio contains further MassTox® Parameter Sets.

For the analysis you require the MassTox® TDM Basic Kit A, the specific MassTox® TDM Parameter Set and the analytical column MassTox® TDM MasterColumn® A.

More Information
Method of Analysis LC-MS/MS
Limit of quantification 1 – 10 µg/l
Linearity up to 50 – 750 μg/l
Recovery 90 – 109 %
Intraassay CV = 2 – 9 %
Interassay CV = 6 – 14 %
Analysis Time 2.8 min
Specimen Serum/Plasma
Sample Preparation
  • Add 800 μl Internal Standard Mix to 12 ml Precipitation Reagent to form mixture A
  • Pipette 50 μl sample/calibrator/MassCheck® control into a 1.5 ml reaction vial
  • Add 25 μl Extraction Buffer, mix briefly (vortex) and incubate 2 min
  • Add 250 μl of mixture A, mix 30 s (vortex) and centrifuge 5 min
  • Dilute the supernatant with Dilution Buffer (depending on the instrument sensitivity) and inject into LC-MS/MS system.
Sample Stability Samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.
Injection Volume 0.2 – 50 µl

0.00 - 0.30 min     20 % Mobile Phase 2
0.31 - 0.60 min     50 % Mobile Phase 2
0.61 - 1.10 min     80 % Mobile Phase 2
1.11 - 2.00 min   100 % Mobile Phase 2
2.01 - 2.21 min,  100 % -> 20 % Mobile Phase 2
2.21 - 2.80 min     20 % Mobile Phase 2

Ionisation ESI positive
Additional Info We recommend to set the scan time to a value that allows to achieve a minimum of 10 data points over the whole peak width.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter Alprazolam, Bromazepam, Clonazepam, Desalkylflurazepam, Flunitrazepam, Flurazepam, Lorazepam, Lormetazepam, Nitrazepam, Triazolam
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