One Step Vitamins A and E in Serum/Plasma - HPLC
With ready-to-use pre-mixed reaction tubes
Only one pipetting step
Fully automated workflow available
CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR-2022 regulation
Vitamins A and E are fat-soluble vitamins, which perform important functions in the body.
Vitamin A plays an important role in vision, bone metabolism, and the synthesis of steroid hormones. Deficiency of vitamin A can lead to night blindness, skin dehydration, and hair loss.
Vitamin E protects, among others, unsaturated fatty acids in the cell membranes and the LDL cholesterol from attacks by reactive radicals, which can occur as a result of increased oxidative stress in the body. Deficiency of vitamin E can lead to permanent joint pain, making the determination of vitamin E levels essential in this case.
However, overdoses of these vitamins may cause hypervitaminosis with intoxication symptoms. In addition, chemotherapies should not be "accompanied" by extra vitamin supplements – especially high doses of vitamins A and E may limit the success of treatment.
- Extremely simple sample preparation in a single step
- Easy handling
- Low analysis costs
- Automated version available
This Chromsystems kit allows the simultaneous analysis of vitamins A and E in serum/plasma in a single isocratic HPLC run with UV detection. The special kit feature: Sample preparation is performed very fast and simply in one single pipetting step using premixed tubes. Additionally, a stable internal standard is used for precise quantification.
Chromsystems offers additional kits for the fast and efficient determination of vitamins A and E. You can choose between several options depending on the device capabilities (HPLC or UHPLC) and the sample quantities in your laboratory. Alternatively, the standard kit 34000 is available, as well as the 34900/UHPLC kit for higher sample throughput using UHPLC determination. Our kits are also available with automated workflow (34700/F and 34900/F).
|Method of Analysis||HPLC|
|Number of Tests||100|
|Please note||The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.|
|Limit of Quantification||Vitamin A: 0.02 mg/l
Vitamin E: 0.60 mg/l
Vitamin A: up to 4.5 mg/l
|Recovery||Vitamin A > 99 %; Vitamin E > 96 %|
Vitamin A: CV ≤ 4.9 %
Vitamin E: CV ≤ 4.1 %
Vitamin A: CV ≤ 5.2 %
Vitamin E: CV ≤ 3.8 %
|Analysis Time||approx. 9 min|
Time of sample drawing
Collection and storage of specimens
|Sample Stability||Protect prepared samples from light. At +2 to +8°C stable up to 48 h and at +20 to +25 °C up to 24 h. For longer storage (max. 3 weeks) keep the samples below -18 °C.|
|Injection Volume||50 µl|
|Flow Rate||1.50 ml/min|
|Column Temperature||ambient (~ 25 °C)|
325 nm, after 3.5 min switch to 295 nm
For the analysis of vitamins A and E any isocratic HPLC system with UV detector is suitable. As these vitamins are detected at different wavelengths, a detector with programmable wavelength switching is very useful for simultaneous analysis of vitamins A and E in a single HPLC run.
|Parameter||Vitamin A, Vitamin E|