Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC

Order No.: 26000, for 100 tests
Parameters:
N-Desmethylolanzapine, Olanzapine, Quetiapine

Low limit of quantification
Robust UV-VIS detection
Metabolite included

CE-IVD validated product ready for IVDR within timeframes and transition periods specified by the IVDR 2017/746

Olanzapine

N-Desmethylolanzapine

Quetiapine

Clinical relevance

Olanzapine and quetiapine are highly effective antipsychotic agents used to treat schizophrenic psychosis. Like clozapine, they are both atypical neuroleptics which are characterised by low levels of side effects in the extrapyramidal motor system (dyskinesia, Parkinson’s disease). Quetiapine is structurally related to olanzapine and is approved as a neuroleptic, but it shows a different receptor profile.

It is essential that the serum levels of these psychotropic drugs be monitored to ensure an optimal therapeutic effect and to minimise any undesirable side effects resulting from overdose. Furthermore, patient compliance gets checked and confirmed by this procedure.

 

Product advantages

  • Low limit of quantification
  • Robust UV-VIS detection
  • Metabolite analysis included

This assay can be used to reliably determine the levels of olanzapine, its metabolite desmethylolanzapine, and quetiapine in serum or plasma. The sample preparation is based on an efficient and selective solid phase extraction including an internal standard. The chromatographic separation is run on an isocratic HPLC system with UV-VIS detector. When used with additional specific calibrators and controls, perazine and citalopram can also be determined in serum or plasma with this kit.

Our TDM Parameter Set Neuroleptics 1/XT from the MassTox® TDM Series A is available as an alternative for the measurement of these analytes using LC-MS/MS.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation

Olanzapine/Desmethylolanzapine: approx. 3.5 ng/ml

Quetiapine: approx. 11 ng/ml

Upper Limit of Quantification

Olanzapine/Desmethylolanzapine: up to 1000 ng/ml
Quetiapine: up to 5000 ng/ml

Recovery Olanzapine/Desmethylolanzapine: 95 %; Quetiapine: 96 %
Intraassay

Olanzapine/Desmethylolanzapine: CV < 4.8 %
Quetiapine: CV < 4.1 %

Interassay

Olanzapine/Desmethylolanzapine: CV < 9.6 %
Quetiapine: CV < 7.2 %

Specimen Serum/Plasma
Sample Preparation

Extraction

  • Equilibrate the sample clean up columns with 1 ml Equilibration Buffer 1 following by 1 ml Equilibration Buffer 2.
  • Apply 1 ml sample and then 100 µl Internal Standard to the sample clean up column, draw through completely by centrifugation or suction, discard effluent.
  • Apply 1 ml Wash Buffer, draw through by centrifugaton or suction, discard effluent, repeat once.
  • Elute with 400 µl Elution Buffer.
  • Inject 50 µl eluate into the HPLC system.
Run Time 18 min
Injection Volume 50 µl
Flow Rate 0.6 ml/min
Column Temperature ambient (~ 25 °C)
Wavelength 254 nm
Gradient isocratic
Additional Info Any isocratic HLPC system with UV-VIS detector is suitable.
Parameters N-Desmethylolanzapine, Olanzapine, Quetiapine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
General lab equipment (non-CE-IVD products), available from Chromsystems:
As a customer please login or register to gain full access.

Olanzapine

N-Desmethylolanzapine

Quetiapine

Clinical relevance

Olanzapine and quetiapine are highly effective antipsychotic agents used to treat schizophrenic psychosis. Like clozapine, they are both atypical neuroleptics which are characterised by low levels of side effects in the extrapyramidal motor system (dyskinesia, Parkinson’s disease). Quetiapine is structurally related to olanzapine and is approved as a neuroleptic, but it shows a different receptor profile.

It is essential that the serum levels of these psychotropic drugs be monitored to ensure an optimal therapeutic effect and to minimise any undesirable side effects resulting from overdose. Furthermore, patient compliance gets checked and confirmed by this procedure.

 

Product advantages

  • Low limit of quantification
  • Robust UV-VIS detection
  • Metabolite analysis included

This assay can be used to reliably determine the levels of olanzapine, its metabolite desmethylolanzapine, and quetiapine in serum or plasma. The sample preparation is based on an efficient and selective solid phase extraction including an internal standard. The chromatographic separation is run on an isocratic HPLC system with UV-VIS detector. When used with additional specific calibrators and controls, perazine and citalopram can also be determined in serum or plasma with this kit.

Our TDM Parameter Set Neuroleptics 1/XT from the MassTox® TDM Series A is available as an alternative for the measurement of these analytes using LC-MS/MS.

More Information
Method of Analysis HPLC
Number of Tests 100
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Lower Limit of Quantitation

Olanzapine/Desmethylolanzapine: approx. 3.5 ng/ml

Quetiapine: approx. 11 ng/ml

Upper Limit of Quantification

Olanzapine/Desmethylolanzapine: up to 1000 ng/ml
Quetiapine: up to 5000 ng/ml

Recovery Olanzapine/Desmethylolanzapine: 95 %; Quetiapine: 96 %
Intraassay

Olanzapine/Desmethylolanzapine: CV < 4.8 %
Quetiapine: CV < 4.1 %

Interassay

Olanzapine/Desmethylolanzapine: CV < 9.6 %
Quetiapine: CV < 7.2 %

Specimen Serum/Plasma
Sample Preparation

Extraction

  • Equilibrate the sample clean up columns with 1 ml Equilibration Buffer 1 following by 1 ml Equilibration Buffer 2.
  • Apply 1 ml sample and then 100 µl Internal Standard to the sample clean up column, draw through completely by centrifugation or suction, discard effluent.
  • Apply 1 ml Wash Buffer, draw through by centrifugaton or suction, discard effluent, repeat once.
  • Elute with 400 µl Elution Buffer.
  • Inject 50 µl eluate into the HPLC system.
Run Time 18 min
Injection Volume 50 µl
Flow Rate 0.6 ml/min
Column Temperature ambient (~ 25 °C)
Wavelength 254 nm
Gradient isocratic
Additional Info Any isocratic HLPC system with UV-VIS detector is suitable.
Parameters N-Desmethylolanzapine, Olanzapine, Quetiapine
The following components are included in the kit:
The following products are not included in the kit but are required for the application of the method:
General lab equipment (non-CE-IVD products), available from Chromsystems:
As a customer please login or register to gain full access.