Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC

Order No.: 26000, for 100 tests
Parameters:
N-Desmethylolanzapine, Olanzapine, Quetiapine

Low limit of quantification
Robust UV-VIS detection
Metabolite included

Olanzapine

N-Desmethylolanzapine

Quetiapine

Clinical relevance

Olanzapine and quetiapine are highly effective antipsychotic agents used to treat schizophrenic psychosis. Like clozapine, they are both atypical neuroleptics which are characterised by low levels of side effects in the extrapyramidal motor system (dyskinesia, Parkinson’s disease). Quetiapine is structurally related to olanzapine and is approved as a neuroleptic, but it shows a different receptor profile.

It is essential that the serum levels of these psychotropic drugs be monitored to ensure an optimal therapeutic effect and to minimise any undesirable side effects resulting from overdose. Furthermore, patient compliance gets checked and confirmed by this procedure.

 

Product advantages

  • Low limit of quantification
  • Robust UV-VIS detection
  • Metabolite analysis included

This kit can be used to reliably determine the levels of olanzapine, its metabolite desmethylolanzapine, and quetiapine in serum or plasma. The sample preparation is based on an efficient and selective solid phase extraction including an internal standard. The chromatographic separation is run on an isocratic HPLC system with UV-VIS detector. When used with additional specific calibrators and controls, perazine and citalopram can also be determined in serum or plasma with this kit.

Our TDM Parameter Set Neuroleptics 1/XT from the MassTox® TDM Series A is available as an alternative for the measurement of these analytes using LC-MS/MS.

More Information
Method of Analysis HPLC
Number of Tests 100
Limit of quantification

Olanzapine/Desmethylolanzapine: approx. 3.5 ng/ml

Quetiapine: approx. 11 ng/ml

Linearity

Olanzapine/Desmethylolanzapine: up to 1000 ng/ml
Quetiapine: up to 5000 ng/ml

Recovery Olanzapine/Desmethylolanzapine: 95 %; Quetiapine: 96 %
Intraassay

Olanzapine/Desmethylolanzapine: CV < 4.8 %
Quetiapine: CV < 4.1 %

Interassay

Olanzapine/Desmethylolanzapine: CV < 9.6 %
Quetiapine: CV < 7.2 %

Analysis Time 18 min
Specimen Serum/Plasma
Pre-analytic Treatment Patient samples are stable cooled (+4 °C) in the dark for at least 24 h. For longer storage keep samples at approx. -20 °C.
Sample Preparation

Extraction

  • Equilibrate the sample clean up columns with 1 ml Equilibration Buffer 1 following by 1 ml Equilibration Buffer 2.
  • Apply 1 ml sample and then 100 µl Internal Standard to the sample clean up column, draw through completely by centrifugation or suction, discard effluent.
  • Apply 1 ml Wash Buffer, draw through by centrifugaton or suction, discard effluent, repeat once.
  • Elute with 400 µl Elution Buffer.
  • Inject 50 µl eluate into the HPLC system.
Sample Stability In glass vials and protected from light, the prepared samples are stable at room temperature (+18 to +30 °C) or refrigerated (+2 to +8 °C) for at least 14 days. Below -18 °C only quetiapin is stable.
Injection Volume 50 µl
Flow rate 0.6 ml/min
Column temperature ambient (~ 25 °C)
Wavelength 254 nm
Gradient isocratic
Additional Info Any isocratic HLPC system with UV-VIS detector is suitable.
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Parameter N-Desmethylolanzapine, Olanzapine, Quetiapine
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