Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC

Order No.: 26000, for 100 tests
Parameters:
N-Desmethylolanzapine, Olanzapine, Quetiapine

Low limit of quantification
Robust UV-VIS detection
Metabolite included

Olanzapine

N-Desmethylolanzapine

Quetiapine

Clinical relevance

Olanzapine and quetiapine are highly effective antipsychotic agents used to treat schizophrenic psychosis. Like clozapine, they are both atypical neuroleptics which are characterised by low levels of side effects in the extrapyramidal motor system (dyskinesia, Parkinson’s disease). Quetiapine is structurally related to olanzapine and is approved as a neuroleptic, but it shows a different receptor profile.

It is essential that the serum levels of these psychotropic drugs be monitored to ensure an optimal therapeutic effect and to minimise any undesirable side effects resulting from overdose. Furthermore, patient compliance gets checked and confirmed by this procedure.

 

Product advantages

  • Low limit of quantification
  • Robust UV-VIS detection
  • Metabolite analysis included

This kit can be used to reliably determine the levels of olanzapine, its metabolite desmethylolanzapine, and quetiapine in serum or plasma. The sample preparation is based on an efficient and selective solid phase extraction including an internal standard. The chromatographic separation is run on an isocratic HPLC system with UV-VIS detector. When used with additional specific calibrators and controls, perazine and citalopram can also be determined in serum or plasma with this kit.

Our TDM Parameter Set Neuroleptics 1/XT from the MassTox® TDM Series A is available as an alternative for the measurement of these analytes using LC-MS/MS.

More Information
Number of Tests 100
Limit of quantification

Olanzapine/Desmethylolanzapine: approx. 3.5 ng/ml

Quetiapine: approx. 11 ng/ml

Linearity

Olanzapine/Desmethylolanzapine: up to 1000 ng/ml
Quetiapine: up to 5000 ng/ml

Recovery Olanzapine/Desmethylolanzapine: 95 %; Quetiapine: 96 %
Intraassay

Olanzapine/Desmethylolanzapine: CV < 4.8 %
Quetiapine: CV < 4.1 %

Interassay

Olanzapine/Desmethylolanzapine: CV < 9.6 %
Quetiapine: CV < 7.2 %

Analysis Time 18 min
Sample Preparation

Extraction

  • Equilibrate the sample clean up columns with 1 ml Equilibration Buffer 1 following by 1 ml Equilibration Buffer 2.
  • Apply 1 ml sample and then 100 µl Internal Standard to the sample clean up column, draw through completely by centrifugation or suction, discard effluent.
  • Apply 1 ml Wash Buffer, draw through by centrifugaton or suction, discard effluent, repeat once.
  • Elute with 400 µl Elution Buffer.
  • Inject 50 µl eluate into the HPLC system.
Sample Stability In glass vials and protected from light, the prepared samples are stable at room temperature (+18 to +30 °C) or refrigerated (+2 to +8 °C) for at least 14 days. Below -18 °C only quetiapin is stable.
Specimen Serum/Plasma
Pre-analytic Treatment Patient samples are stable cooled (+4 °C) in the dark for at least 24 h. For longer storage keep samples at approx. -20 °C.
Injection Volume 50 µl
Flow rate 0.6 ml/min
Column temperature ambient (~ 25 °C)
Wavelength 254 nm
Gradient isocratic
Please note The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.
Method of Analysis HPLC
Parameter N-Desmethylolanzapine, Olanzapine, Quetiapine
Complete Specification

Olanzapine/Desmethylolanzapine

Linearity: up to 1000 ng/ml
Limit of quantification: aproxx.* 4 ng/ml
(therapeutical range: 20-80 ng/ml)
Intra-assay: CV < 5 %
Inter-assay: CV < 6 %
Recovery: 95 %

Quetiapine

Linearity: up to 3000 ng/ml
Limit of quantification: aproxx.* 11 ng/ml
(therapeutical range: 40-400 ng/ml)
Intra-assay: CV < 4 %
Inter-assay: CV < 4 %
Recovery: 97 %
Run time: approx. 18 min

*depending on the detector employed

HPLC parameters

For the Chromsystems HPLC analysis of olanzapine/ desmethylolanzapine any isocratic HPLC system with electrochemical detector is suitable.

Injection volume: 50 µl
Flow rate: 0.6 ml/min
Wavelength: 254 nm
Column temperature: ambient (~25 °C)
Additional Info Any isocratic HLPC system with UV-VIS detector is suitable.
  1. 26001 HPLC mobile phase olanzapine desmethylolanzapine serum plasma
    Mobile Phase
    Order no.: 26001
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  2. 26001 HPLC mobile phase olanzapine desmethylolanzapine serum plasma
    Mobile Phase, 10 x 1000 ml
    Order no.: 26002
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  3. 26004 HPLC internal standard olanzapine desmethylolanzapine serum plasma
    Olanzapine/Desmethylolanzapine Plasma Calibration Standard
    Order no.: 26003
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  4. 26004 HPLC internal standard olanzapine desmethylolanzapine serum plasma
    Internal Standard
    Order no.: 26004
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  5. 26005 HPLC equilibration buffer 1 olanzapine desmethylolanzapine serum plasma
    Equilibration Buffer 1
    Order no.: 26005
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  6. 26006 HPLC equilibration buffer 2 olanzapine desmethylolanzapine serum plasma
    Equilibration Buffer 2
    Order no.: 26006
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  7. 26009 HPLC wash buffer olanzapine desmethylolanzapine serum plasma
    Wash Buffer
    Order no.: 26009
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  8. 26010 HPLC elution buffer olanzapine desmethylolanzapine serum plasma
    Elution Buffer
    Order no.: 26010
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  9. 26008 HPLC sample clean up columns olanzapine desmethylolanzapine
    Sample Clean Up Columns
    Order no.: 26008
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  1. 26004 HPLC internal standard olanzapine desmethylolanzapine serum plasma
    Olanzapine/Desmethylolanzapine Plasma Calibration Standard
    Order no.: 26003
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  2. 28007 HPLC Perazine, Quetiapine, Citalopram Plasma Calibration Standard
    Perazine, Quetiapine, Citalopram Plasma Calibration Standard
    Order no.: 28007
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
  1. PEEK Prefilter Housing
    PEEK Prefilter Housing
    Order no.: 15010
    Details
  2. PEEK-encased Prefilter, 2 µm
    Details
  3. 26100 HPLC column olanzapine desmethylolanzapine serum plasma
    HPLC column
    Order no.: 26100
    Olanzapine and Desmethylolanzapine in Serum/Plasma
    Details
  4. PEEK-encased Prefilter, 5 µm
    Details
  5. Precolumn Cartridge Holder 4/10
    Details
  6. Precolumn cartridge 4/10
    Precolumn cartridge 4/10
    Order no.: 18026
    Olanzapine and Desmethylolanzapine in Serum/Plasma - HPLC
    Details
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